##gff-version 3
##sequence-region BBa_K1997024 1 5180
BBa_K1997024	.	engineered_region	1	223	.	+	0	ID=BBa_K1789012;Name=BBa_K1789012
BBa_K1997024	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_K1789002;Name=BBa_K1789002;Parent=BBa_K1789012
BBa_K1997024	.	ribosome_entry_site	209	223	.	+	0	ID=annotation2454064;Name=RBS30;Parent=BBa_K1789002
BBa_K1997024	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1789012
BBa_K1997024	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1997024	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1997024	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1997024	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1997024	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1997024	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1997024	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1997024	.	terminator	5052	5180	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1997024	.	terminator	5052	5131	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1997024	.	engineered_region	5052	5131	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1997024	.	stem_loop	5063	5106	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1997024	.	terminator	5140	5180	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1997024	.	engineered_region	5140	5180	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1997024	.	stop_codon	5173	5173	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1997024	.	stem_loop	5147	5166	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1997024	.	polyA_site	5167	5180	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1997024	.	CDS	940	5043	.	+	0	ID=BBa_K1997010;Name=BBa_K1997010
BBa_K1997024	.	CDS	940	5043	.	+	0	ID=annotation2513783;Name=dCas9;Parent=BBa_K1997010
BBa_K1997024	.	CDS	232	933	.	+	0	ID=BBa_K1997000;Name=BBa_K1997000
BBa_K1997024	.	CDS	232	261	.	+	0	ID=annotation2496346;Name=10aa linker;Parent=BBa_K1997000
BBa_K1997024	.	CDS	262	903	.	+	0	ID=annotation2496347;Name=sHRP-N;Parent=BBa_K1997000
BBa_K1997024	.	CDS	904	933	.	+	0	ID=annotation2496348;Name=10aa linker;Parent=BBa_K1997000
>BBa_K1997024
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagagaaaggt
tctggttctacctctggttctggtatgcagttaacccctacattctacgacaatagctgtcccaacgtgtccaacatcg
ttcgcgacattatcgtcaacgagctcagatccgatcccaggatcgctgcttcaatattacgtctgcacttccatgactg
cttcgtgaatggttgcgacgctagcatattactggacaacaccaccagtttccgcactgaaaaggatgcattcgggaac
gctaacagcgccaggggctttagcgtgatcgatcgcatgaaggctgccgttgagtcagcatgcccaggtacagtcagtt
gtgcagacctgctgactatagctgcgcaacagagcgtgactcttgcaggcggaccgtcctggagagtgccgctcggtcg
acgtgactccctacaggcattcctagatctggccaacgccaacttgcctgctccattcttcaccctgccccagctgaag
gatagctttagaaacgtgggtctgaatcgctcaagtgaccttgtggctctgtccggaggacacacatttggaaagagcc
agtgtaggttcatcatggataggctctacaatttcagcaacactgggttacctgaccccacgctgaacactacgtatct
ccagacactgagaggcttgtgcccactgaatggcaaaggttctggttctacctctggttctggttactagatggataag
aaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtcta
aaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtgg
agagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatcta
caggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaag
aagacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactat
ctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatg
attaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagt
tggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgc
acgattgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaat
ctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagcttt
caaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatgctgatttgtttttggcagc
taagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagct
tcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagaaa
agtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaatt
ttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttg
ctgcgcaagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaa
gacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgacttccttatta
tgttggtccattggcgcgtggcaatagtcgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaat
tttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaa
atgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtttataacgaattgacaaaggtcaaatatgt
tactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaat
cgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatttcaggag
ttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataa
tgaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaaga
cttaaaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtt
tgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggttt
tgccaatcgcaattttatgcagctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtct
ggacaaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactg
taaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgttattgaaatggcacgtgaaaa
tcagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagt
cagattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaa
gagacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagttt
ccttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttccaagt
gaagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgata
atttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcg
ccaaatcactaagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcga
gaggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgaga
ttaacaattaccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaact
tgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggc
aaagcaaccgcaaaatatttcttttactctaatatcatgaacttcttcaaaacagaaattacacttgcaaatggagaga
ttcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagt
gcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcggattctccaaggagtca
attttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgata
gtccaacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaaga
gttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataag
gaagttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctgg
ctagtgccggagaattacaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtca
ttatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagat
gagattattgagcaaatcagtgaattttctaagcgtgttattttagcagatgccaatttagataaagttcttagtgcat
ataacaaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagc
tcccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaagaagttttagatgccact
cttatccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgactactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata