LOCUS BBa_K1997025 4823 bp DNA linear 30-Dec-2025 DEFINITION P+R->sHRP-C->dCas9->Ter ACCESSION BBa_K1997025 VERSION BBa_K1997025.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS30 RBS 209..223 /label=BBa_K1789002 misc_feature 1..223 /label=BBa_K1789012 CDS 232..261 /label=10aa linker CDS 262..546 /label=sHRP-C CDS 547..576 /label=10aa linker CDS 232..576 /label=BBa_K1997001 CDS 583..4686 /label=dCas9 CDS 583..4686 /label=BBa_K1997010 stem_loop 4706..4749 /label=stem_loop misc_feature 4695..4774 /label=BBa_B0010 terminator 4695..4774 /label=BBa_B0010 stem_loop 4790..4809 /label=T7 TE misc_feature 4816..4816 /label=stop polyA_site 4810..4823 /label=polya misc_feature 4783..4823 /label=BBa_B0012 terminator 4783..4823 /label=BBa_B0012 terminator 4695..4823 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga gaaaggttct 241 ggttctacct ctggttctgg taacctcagt gcactggtgg actttgatct gcggacccca 301 accatcttcg ataacaagta ctatgtgaat ttagaagagc agaaaggcct gatacagagt 361 gatcaagaac tgtttagcag tccagatgcc actgacacca tcccactggt gagaagtttt 421 gctaactcta ctcaaacctt ctttaacgcc ttcgtggaag ccatggaccg tatgggtaac 481 attacccctc tgacgggtac ccaaggccag attcgtcgca actgtagagt ggtcaacagc 541 aactctaaag gttctggttc tacctctggt tctggttact agatggataa gaaatactca 601 ataggcttag ctatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag 661 gttccgtcta aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat 721 cttatagggg ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg 781 acagctcgta gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt 841 tcaaatgaga tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg 901 gtggaagaag acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt 961 gcttatcatg agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact 1021 gataaagcgg atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt 1081 cattttttga ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc 1141 cagttggtac aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta 1201 gatgctaaag cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt 1261 gctcagctcc ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg 1321 ggtttgaccc ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt 1381 tcaaaagata cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat 1441 gctgatttgt ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta 1501 agagtaaata ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat 1561 gaacatcatc aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag 1621 tataaagaaa tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga 1681 gctagccaag aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact 1741 gaggaattat tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac 1801 aacggctcta ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa 1861 gaagactttt atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt 1921 cgacttcctt attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact 1981 cggaagtctg aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct 2041 tcagctcaat catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa 2101 gtactaccaa aacatagttt gctttatgag tattttacgg tttataacga attgacaaag 2161 gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa 2221 gccattgttg atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa 2281 gattatttca aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga 2341 tttaatgctt cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt 2401 ttggataatg aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt 2461 gaagataggg agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag 2521 gtgatgaaac agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg 2581 attaatggta ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat 2641 ggttttgcca atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa 2701 gacattcaaa aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat 2761 ttagctggta gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa 2821 ttggtcaaag taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa 2881 aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa 2941 ggtatcaaag aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg 3001 caaaatgaaa agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa 3061 gaattagata ttaatcgttt aagtgattat gatgtcgatg ccattgttcc acaaagtttc 3121 cttaaagacg attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa 3181 tcggataacg ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt 3241 ctaaacgcca agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga 3301 ggtttgagtg aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa 3361 atcactaagc atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat 3421 gataaactta ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc 3481 cgaaaagatt tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat 3541 gcgtatctaa atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg 3601 gagtttgtct atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag 3661 caagaaatag gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc 3721 aaaacagaaa ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat 3781 ggggaaactg gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta 3841 ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc 3901 aaggagtcaa ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg 3961 gatccaaaaa aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt 4021 gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc 4081 acaattatgg aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga 4141 tataaggaag ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta 4201 gaaaacggtc gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg 4261 gctctgccaa gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag 4321 ggtagtccag aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta 4381 gatgagatta ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat 4441 ttagataaag ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca 4501 gaaaatatta ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat 4561 tttgatacaa caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact 4621 cttatccatc aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga 4681 ggtgactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 4741 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 4801 gggtgggcct ttctgcgttt ata //