##gff-version 3
##sequence-region BBa_K1997025 1 4823
BBa_K1997025	.	CDS	232	576	.	+	0	ID=BBa_K1997001;Name=BBa_K1997001
BBa_K1997025	.	CDS	232	261	.	+	0	ID=annotation2496404;Name=10aa linker;Parent=BBa_K1997001
BBa_K1997025	.	CDS	547	576	.	+	0	ID=annotation2496407;Name=10aa linker;Parent=BBa_K1997001
BBa_K1997025	.	CDS	262	546	.	+	0	ID=annotation2496405;Name=sHRP-C;Parent=BBa_K1997001
BBa_K1997025	.	CDS	583	4686	.	+	0	ID=BBa_K1997010;Name=BBa_K1997010
BBa_K1997025	.	CDS	583	4686	.	+	0	ID=annotation2513783;Name=dCas9;Parent=BBa_K1997010
BBa_K1997025	.	engineered_region	1	223	.	+	0	ID=BBa_K1789012;Name=BBa_K1789012
BBa_K1997025	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1789012
BBa_K1997025	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1997025	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1997025	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1997025	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1997025	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1997025	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1997025	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1997025	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_K1789002;Name=BBa_K1789002;Parent=BBa_K1789012
BBa_K1997025	.	ribosome_entry_site	209	223	.	+	0	ID=annotation2454064;Name=RBS30;Parent=BBa_K1789002
BBa_K1997025	.	terminator	4695	4823	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1997025	.	terminator	4783	4823	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1997025	.	polyA_site	4810	4823	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1997025	.	stem_loop	4790	4809	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1997025	.	stop_codon	4816	4816	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1997025	.	engineered_region	4783	4823	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1997025	.	terminator	4695	4774	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1997025	.	stem_loop	4706	4749	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1997025	.	engineered_region	4695	4774	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K1997025
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagagaaaggt
tctggttctacctctggttctggtaacctcagtgcactggtggactttgatctgcggaccccaaccatcttcgataaca
agtactatgtgaatttagaagagcagaaaggcctgatacagagtgatcaagaactgtttagcagtccagatgccactga
caccatcccactggtgagaagttttgctaactctactcaaaccttctttaacgccttcgtggaagccatggaccgtatg
ggtaacattacccctctgacgggtacccaaggccagattcgtcgcaactgtagagtggtcaacagcaactctaaaggtt
ctggttctacctctggttctggttactagatggataagaaatactcaataggcttagctatcggcacaaatagcgtcgg
atgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatc
aaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaagcgactcgtctcaaacggacagctcgta
gaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatag
tttctttcatcgacttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttggaaatata
gtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaag
cggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattgagggagatttaaa
tcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctatt
aacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctc
agctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatc
aaattttgatttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcg
caaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaa
gagtaaatactgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgac
tcttttaaaagctttagttcgacaacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatat
gcaggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggta
ctgaggaattattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggacctttgacaacggctctattcccca
tcaaattcacttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgag
aagattgaaaaaatcttgacttttcgacttccttattatgttggtccattggcgcgtggcaatagtcgttttgcatgga
tgactcggaagtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatt
tattgaacgcatgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtat
tttacggtttataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaac
agaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaa
aaaaatagaatgttttgatagtgttgaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgat
ttgctaaaaattattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacat
tgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgctcacctctttgatgataaggtgatgaa
acagcttaaacgtcgccgttatactggttggggacgtttgtctcgaaaattgattaatggtattagggataagcaatct
ggcaaaacaatattagattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgatagtt
tgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagtttacatgaacatattgcaaatttagc
tggtagccctgctattaaaaaaggtattttacagactgtaaaagttgttgatgaattggtcaaagtaatggggcggcat
aagccagaaaatatcgttattgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgta
tgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcctgttgaaaatactcaattgca
aaatgaaaagctctatctctattatctccaaaatggaagagacatgtatgtggaccaagaattagatattaatcgttta
agtgattatgatgtcgatgccattgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgtt
ctgataaaaatcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaaaactattggagacaact
tctaaacgccaagttaatcactcaacgtaagtttgataatttaacgaaagctgaacgtggaggtttgagtgaacttgat
aaagctggttttatcaaacgccaattggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgca
tgaatactaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatctaaattagtttctga
cttccgaaaagatttccaattctataaagtacgtgagattaacaattaccatcatgcccatgatgcgtatctaaatgcc
gtcgttggaactgctttgattaagaaatatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatg
ttcgtaaaatgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactctaatatcatgaa
cttcttcaaaacagaaattacacttgcaaatggagagattcgcaaacgccctctaatcgaaactaatggggaaactgga
gaaattgtctgggataaagggcgagattttgccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaaga
aaacagaagtacagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttattgctcgtaa
aaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagcttattcagtcctagtggttgctaaggtg
gaaaaagggaaatcgaagaagttaaaatccgttaaagagttactagggatcacaattatggaaagaagttcctttgaaa
aaaatccgattgactttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaatatag
tctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaattacaaaaaggaaatgagctggctctg
ccaagcaaatatgtgaattttttatatttagctagtcattatgaaaagttgaagggtagtccagaagataacgaacaaa
aacaattgtttgtggagcagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgttat
tttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaaccaatacgtgaacaagcagaa
aatattattcatttatttacgttgacgaatcttggagctcccgctgcttttaaatattttgatacaacaattgatcgta
aacgatatacgtctacaaaagaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcat
tgatttgagtcagctaggaggtgactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata