LOCUS BBa_K1998000 10938 bp DNA linear 23-Dec-2024 DEFINITION Mg-Chelatase Plasmid ACCESSION BBa_K1998000 VERSION BBa_K1998000.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 214..224 /label=RBS CDS 233..1321 /label=ChlI1 CDS 209..1324 /label=BBa_K1080000 RBS 1338..1348 /label=RBS CDS 1357..3486 /label=ChlD CDS 1333..3486 /label=BBa_K1080002 RBS 3500..3510 /label=RBS CDS 3519..4169 /label=GUN4 CDS 3495..4222 /label=BBa_K1080003 misc_feature 1..4222 /label=BBa_K1326008 RBS 4236..4246 /label=RBS CDS 4255..5436 /label=ChlI2 CDS 4231..5442 /label=BBa_K1080011 RBS 5456..5466 /label=RBS CDS 5475..6596 /label=CTH1 CDS 5451..6602 /label=BBa_K1080005 CDS 6611..6698 /label=end of LacI coding region (inactive) misc_feature 6611..6810 /label=BBa_R0010 misc_feature 6699..6736 /label=CAP binding site promoter 6747..6752 /label=-35 promoter 6771..6776 /label=-10 misc_feature 6776..6810 /label=LacI binding site misc_feature 6783..6783 /label=start promoter 6611..6810 /label=BBa_R0010 RBS 6819..6829 /label=RBS CDS 6838..10932 /label=ChlH CDS 6819..10938 /label=BBa_K1640019 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagag ctttaagaag gagatataca taatggcagc 241 tactgaggtc aaggctgctg aaggccgtac tgagaaggag ctgggtcagg cacgtccaat 301 ctttccattc accgccatcg ttggtcagga tgagatgaag ctggcgctga ttctgaacgt 361 gatcgatcct aagatcggtg gtgtcatgat catgggtgac cgtggtactg gtaagtccac 421 tactattcgt gcactggcag atctgctgcc tgaaatgcag gtggttgcta acgatccatt 481 taactcggat ccaactgatc cagagctgat gagtgaagaa gttcgtaacc gtgtcaaggc 541 aggtgaacag ctgccagtgt cttccaagaa gattcctatg gttgacctgc cactgggtgc 601 tactgaagat cgtgtgtgcg gtaccatcga tatcgagaag gcactgactg aaggtgttaa 661 ggcattcgag cctggtctgc tggctaaggc taaccgtggt atcctgtacg tggatgaagt 721 caacctgctg gatgatcatc tggttgatgt tctgctggac tcggcagcat ctggttggaa 781 caccgtggag cgtgaaggta tctccatcag ccacccagcc cgcttcatcc tggttggttc 841 gggtaaccca gaggaaggtg aactgcgtcc acagctgctg gatcgcttcg gcatgcacgc 901 tcagatcggt accgtcaaag atcctcgtct gcgtgtgcaa atcgtgtcgc agcgctcgac 961 cttcgacgag aatccagccg cctttcgcaa ggactacgaa gctggtcaga tggcactgac 1021 acagcgtatc gttgacgctc gtaagctgct gaagcaaggt gaagtcaact acgacttccg 1081 cgtcaagatc agccagatct gctcggacct gaacgtggac ggcatccgtg gtgatatcgt 1141 gactaaccgt gcagctaagg ctctggctgc attcgaaggt cgtaccgaag tgactccaga 1201 agacatctac cgtgtcattc cactgtgcct gcgtcaccgt ctccgtaaag acccactggc 1261 tgagatcgac gacggtgacc gtgtgcgtga gatcttcaag caggtgttcg gcatggagta 1321 ataatactag agagctttaa gaaggagata tacataatgc gcgccatgaa ggtgtctgag 1381 gaggactcca agggcttcga tgcggatgtg tcgacccgcc tggcccgctc gtaccctctg 1441 gcggccgtgg tgggccagga caacatcaag caggcgctgc tgctgggcgc cgtggacacc 1501 gggctgggcg gcatcgccat cgccggtcgc cgcggtaccg ccaagtccat catggctcgc 1561 ggcctgcacg ctctgctgcc gcccattgag gtggtggagg gcagcatctg caacgccgac 1621 cccgaggacc cccgctcctg ggaggctggc ctggctgaga agtatgcggg cggccctgtg 1681 aagaccaaga tgcgctcggc gccgtttgtg cagatccctc tgggtgtgac tgaggaccgc 1741 ttggtgggca ctgtggacat tgaggcgtcc atgaaggagg gcaagactgt gttccagccc 1801 ggcctgctgg ctgaggcgca ccgcggcatc ctgtacgtgg acgagatcaa cctgctggat 1861 gacggcattg ccaacctgct gctgtccatc ctgtcggacg gagtcaacgt ggtggagcgc 1921 gagggcatct ccatcagcca cccctgccgg ccgctgctga ttgccaccta caaccccgag 1981 gagggccctc tgcgtgagca cctgctggac cgcatcgcca ttggcctcag cgccgacgtc 2041 cccagcacca gcgacgagcg cgtcaaggcc attgacgcag ccatccgctt ccaggacaag 2101 ccgcaggaca ctattgacga caccgcggag ctcaccgacg ccctgcgcac ctcggtcatc 2161 ctggctcgcg agtacctgaa ggacgtgacc atcgcgccgg agcaggtgac ctacattgtg 2221 gaggaggcgc gccgcggcgg agtccagggg caccgcgcgg agctgtacgc ggtcaagtgt 2281 gccaaggcgt gtgcggctct ggagggccgt gagcgtgtga acaaggatga cctgcgccag 2341 gccgtgcagc tggtcatcct gccgcgcgcc accatcctgg accagccccc gcccgagcag 2401 gagcagcccc cgccgccgcc cccgccccct cccccgccgc cgccgcagga ccaaatggag 2461 gacgaggacc aggaggagaa ggaggacgag aaggaggagg aggagaagga gaacgaggac 2521 caggacgagc ccgagatccc tcaggagttc atgtttgagt ccgagggcgt catcatggac 2581 ccctccatcc tcatgttcgc gcagcagcag cagcgcgcgc agggccgctc cggccgcgcc 2641 aagacgctca tcttcagcga cgaccgcggc cgctacatca agcccatgct gcccaagggt 2701 gacaaggtca agcgcctggc agtggacgcc acgcttcgcg ccgccgcgcc ctaccagaag 2761 attcgccggc agcaggccat cagcgagggc aaggtgcagc gcaaggtgta cgtggacaag 2821 ccagacatgc gctccaagaa gctggcccgc aaggccggtg cgctggtgat ttttgttgtg 2881 gacgcgtccg gctccatggc tctgaaccgc atgagcgccg ccaagggcgc ctgcatgcgc 2941 ctgctggctg agtcgtacac cagccgcgac caggtgtgcc tcatcccctt ctacggcgac 3001 aaggccgagg tgctgctgcc gccctccaag tccatcgcca tggcccgccg ccgcctggac 3061 tcgctgccct gcggcggcgg ctcgcccctt gcgcacggcc tgtccacggc ggtacgtgtg 3121 ggcatgcagg ccagccaggc gggcgaggtg ggccgcgtca tgatggtgct catcacggac 3181 ggccgcgcca acgtcagcct ggccaagtcc aacgaggacc ccgaggcgct caagcccgac 3241 gcgcccaagc ccaccgccga ctcgctgaag gacgaggtgc gcgacatggc caagaaggcc 3301 gcgtccgccg gcatcaacgt gcttgtcatt gacacggaga acaagttcgt gagcaccggc 3361 tttgcggagg agatctccaa ggcagcgcag ggcaagtact actacctgcc caacgccagc 3421 gacgcagcaa tcgcagcagc agcatccggt gcaatggcag cagctaaggg aggatactag 3481 caataatact agagagcttt aagaaggaga tatacataat ggcgatgcgt gtcaccgtcg 3541 ctgctggtaa gctggactct gtctccctgt tcggtggaga caccgccagc ctgatgggtg 3601 gttcacagac tgtggagaag aagaagtctg gtaaggaggc tgtgatggag gtgcagctca 3661 gctccacagc aggcatcgac tacaccgtcc ttcgtgacca ccttgccaac ggtgagttcc 3721 gtgaggcaga ggacgagact cgtgctctgc tgatcaagct tgctggtcca gaagcagtca 3781 agcgcaactg ggtttacttc accgaggtga agaacatttc cgtcaccgat ttccagaccc 3841 tggacaacct gtggaaggcg tccagcaaca acaagttcgg ctacagcgtt cagaaggaga 3901 tctgggtgca gaaccagaag cgctggccca agttcttcaa gcagattgac tggacccagg 3961 gcgagaacaa caactaccgg aagtggccca tggagttcat ctacagcatg gacgcccccc 4021 gcggccacct gcccctcacc aacgcgctcc gagggacgca gctgttccag gcgatcatgg 4081 agcacccagc gttcgagaag tccagcacgg caaagacgct tgatcagaag gcggcggagg 4141 ccgccgggcg gacacagtcg ctgttctaag ctctcagcag ctcagcggct gatcctgcca 4201 ggtgttgtat cagcggccgc cttactagag agctttaaga aggagatata cataatgcca 4261 tccacgaaag cagctaagaa gccgaacttc ccattcgtca agattcaagg tcaagaggaa 4321 atgaagcttg cactgctgct gaacgttgtt gatccaaaca tcggtggtgt acttattatg 4381 ggtgaccgtg gtactgctaa gtcggtcgca gtccgtgctc tggtggatat gcttccagac 4441 attgacgtgg ttgaaggtga cgcattcaac agctctccaa cagatcctaa gttcatgggt 4501 ccagacacac tgcaacgttt ccgtaacggc gagaagctgc caaccgttcg tatgcggaca 4561 ccactggttg aactgcctct gggtgcaacc gaggatcgta tctgcggtac aatcgatatc 4621 gagaaggcac tgacgcaggg tatcaaggca tacgaacctg gtctgctggc aaaggctaac 4681 cgtggtatcc tgtatgtgga cgaagtgaac ctgctggatg atggtctggt tgatgtcgtg 4741 ctggactcgt cggctagcgg tctgaacact gtggaacgtg aaggtgtgtc tattgtgcat 4801 cctgcacgtt tcatcatgat tggttcaggt aacccccagg aaggtgaact gcgtccacag 4861 ctgctggatc gtttcggcat gagcgtcaac gtggcaacgc tgcaagacac caagcaacgt 4921 acgcagctgg tgctggatcg tcttgcgtac gaagctgacc ctgacgcatt tgtggactcg 4981 tgcaaggcag agcagacagc actcacggac aagctggaag cagcacgtca acgtctgcgt 5041 tccgtcaaga tcagcgaaga actgcaaatc ctgatctctg acatttgctc tcgtctggat 5101 gttgatggtc tgcgtggtga cattgttatc aaccgtgctg ctaaggctct tgttgcattc 5161 gaaggtcgta ccgaagttac cacgaatgac gtggaacgtg tcatctcggg ttgcctcaac 5221 catcgtctgc gtaaggaccc actggacccc attgacaacg gcactaaggt tgcaatcctg 5281 ttcaagcgta tgactgatcc agaaatcatg aagcgtgaag aagaagctaa gaagaagcgc 5341 gaagaggctg ctgccaaggc taaggctgaa ggtaaggctg atcgtcctac gggtgctaag 5401 gctggtgcat gggctggctt gccacctcgt cggtaataat aatactagag agctttaaga 5461 aggagatata cataatggtg gctgctaccg ctgctcccca ggaggttgag ggcttcaagg 5521 ttatgcgtga cggcatcaag gttgcttccg atgagaccct gctcactcct cgcttctaca 5581 ccaccgactt cgatgagatg gagcgtctgt tcagcctgga gctgaacaag aacatggaca 5641 tggaggagtt cgaggcgatg ctgaacgagt tcaagctgga ctacaaccag cgtcacttcg 5701 tccgcaacga gaccttcaag gaggcagcgg agaagattca gggtcccacc cgcaagatct 5761 tcattgagtt cctggagcgc tcgtgcactg ctgagttctc gggtttcctg ctctacaagg 5821 agctgggtcg tcgtctgaag gccacgaacc ctgtggtcgc ggagatcttc accctgatgt 5881 cccgtgacga ggcacgtcac gctggcttcc tgaacaaggc catgtccgac ttcaacctgg 5941 ctctggacct gggtttcctg accaagaacc gcaagtacac cttcttcaag cccaagttca 6001 tcttctacgc cacttacctg tcggagaaga ttggctactg gcgttacatt tctatctacc 6061 gtcacctgca acgcaaccca gacaaccagc tgtatccgct gttcgagtac ttcgagaact 6121 ggtgccagga cgagaaccgt cacggtgact tcttcaccgc agtgctgaag gcacgtccgg 6181 agatggtcaa cgactgggct gccaagctgt ggtcgcgctt cttctgcctg tcggtgtaca 6241 tcaccatgta cctgaacgac caccagcgtg acgcattcta cagctctctt ggactgaaca 6301 ccacacagtt caaccagcac gtgatcatcg agaccaacaa gtctactgag cgtatcttcc 6361 ccgctgtgcc ggatgtggag aaccccgagt tcttccgtcg tatggacctg ctggtcaagt 6421 acaacgcaca gctggtgaac attggaagca tgaacctgcc aagtccaatc aaggctatca 6481 tgaaggcacc aattctggag cgcatggtcg ctgaggtgtt ccaggtattc atcatgacac 6541 ctaaggagtc tggctcgtac gacctggatg ccaacaagac cgctctggtc tactaataat 6601 aatactagag caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 6661 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 6721 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 6781 ggaattgtga gcggataaca atttcacaca tactagagag aagaaggaga tatacatatg 6841 tgtaatgttg cgaccggtcc ccgtccccca atgaccactt ttaccggtgg aaacaaaggc 6901 ccggcgaaac agcaggttag cctggacctg cgtgatgatg gtgcaggcat gtttacctct 6961 acaagccctg agatgcgccg tgttgtacca gatgatgtta aaggtcgcgt aaaagtcaag 7021 gtagtatatg tggtgctgga ggcgcaatat cagagcgcta tttccgcggc ggtgaaaaat 7081 atcaacgcga aaaattccaa ggtatgtttc gaagtcgtgg gctacctgct ggaggaactc 7141 cgcgaccaga aaaatttaga catgttaaag gaggatgtcg cgagcgctaa catttttatc 7201 ggcagtttga tttttattga agagctggca gaaaagatcg tggaggccgt cagccccctg 7261 cgtgagaaat tggacgcttg cttgatcttc ccctccatgc cagctgttat gaagttaaac 7321 aaactgggca cgttttctat ggcacagctt ggtcaaagca aaagtgtttt cagcgaattt 7381 attaaatctg cccgtaaaaa caatgacaac tttgaggagg gtctgttaaa actggttcgc 7441 actttgccca aagtgctgaa gtacctgcct agcgataaag cgcaagatgc gaagaacttt 7501 gttaatagtt tgcagtattg gctgggtggc aactctgata acttggaaaa tctgttgtta 7561 aacaccgttt ccaactacgt tccggctctg aaaggcgttg atttttccgt ggccgaaccg 7621 acggcctatc cggatgttgg tatctggcat ccactggcat ccggaatgta cgaagatctc 7681 aaagaatatc tgaattggta tgatacccgt aaagatatgg tttttgctaa agatgcccct 7741 gtgattggct tagtcctcca gcgttctcat ttagtcaccg gtgacgaggg ccattattct 7801 ggtgtggtgg cagagctgga gtcgcgcggc gctaaggtta tcccagtttt tgcgggtggt 7861 ttagatttta gcgcgccagt gaaaaaattc ttttatgatc ccttgggtag tgggcgcacc 7921 ttcgtggata ccgtcgtctc actgaccggt ttcgcactgg ttggggggcc ggcgcgtcaa 7981 gacgccccga aagcaattga agcgctgaaa aacctcaatg tgccgtatct ggttagtctg 8041 ccgctcgtat ttcagaccac ggaagaatgg ttagattcag agctgggtgt acatccagtg 8101 caagtggcat tgcaggtagc cctgcctgaa ctggatggcg cgatggaacc gattgttttt 8161 gcgggtcgtg attctaacac cggcaaaagt cactcacttc cagatcgtat tgcgtccctg 8221 tgtgcccgcg cggtcaattg ggcgaacctg cgtaagaaac gcaacgcgga aaaaaaatta 8281 gcggtaaccg ttttctcctt tccgccggat aagggtaacg tgggtaccgc ggcctactta 8341 aatgtatttg gctcaattta ccgcgtactc aaaaaccttc agcgcgaagg gtatgatgtt 8401 ggtgcccttc cgcctagtga ggaagatctt atccaaagtg tcctgactca gaaagaggct 8461 aaattcaatt caaccgacct gcacattgca tacaaaatga aagttgacga gtatcagaag 8521 ctttgcccgt atgcagaagc gctggaagaa aactggggta agcctccagg cacacttaac 8581 accaatggcc aagagctgct ggtatatggg cgtcaatacg gcaacgtgtt cattggtgtg 8641 cagcctacct ttggttatga gggcgatccg atgcgtctgc ttttttcaaa gtccgcgtcc 8701 ccacatcacg gtttcgctgc ttattacacg tttctggaaa aaattttcaa agcagatgct 8761 gtgctccact tcgggaccca tgggtccctg gaatttatgc ccggtaaaca ggtcggcatg 8821 tctggtgtat gttatccgga ttcactgatt ggcactattc caaacctcta ttattatgca 8881 gccaacaatc caagtgaggc aaccattgcc aaacgtcgca gctacgcaaa tacgatctct 8941 tatctgaccc caccggcaga gaatgcaggt ctgtacaagg ggttaaaaga actgaaagaa 9001 cttattagta gctatcaagg aatgcgcgaa agcggtcgcg cggagcagat ctgtgcgacg 9061 attattgaaa cggctaaact gtgtaacttg gatcgcgacg tgacgctccc ggatgccgac 9121 gccaaagatc tgaccatgga catgcgtgat tctgtggtgg gccaagttta ccgtaagctg 9181 atggagatcg aatcacgcct gctgccgtgc ggtttgcacg tggttggctg tcctccgact 9241 gctgaagagg ccgtcgctac tttagtcaac attgcagaac tggaccgccc agacaacaac 9301 ccaccgatta aagggatgcc ggggattctt gcgcgtgcta ttggccgcga tattgaaagc 9361 atttactccg gtaacaacaa gggcgtcctg gctgacgttg accagctgca acgcatcacc 9421 gaggcctccc gtacctgcgt gcgtgagttc gtgaaggacc gcaccggtct gaacggtcgt 9481 atcggcacca actggatcac caacctgctc aagttcaccg gtttctacgt ggacccctgg 9541 gtgcgtggtc tgcaaaacgg tgagttcgcc agcgcaaacc gtgaggagct gatcaccctg 9601 ttcaactacc tggagttctg cctgacccag gtggtcaagg acaacgagct gggtgcactg 9661 gtagaggcac tgaacggtca gtacgtcgag ccaggtccag gtggtgaccc catccgcaac 9721 cccaacgtgc tgcccaccgg taagaacatc cacgcactgg accctcagtc gattcccact 9781 caggcagcac tgaagagcgc acgtctggtg gtggaccgtc tgctggaccg tgagcgcgac 9841 aacaacggcg gcaagtaccc cgagaccatc gcgctggtgc tgtggggcac tgacaacatc 9901 aagacctacg gcgagtcgct ggcccaggtc atgatgatgg tcggtgtcaa gcccgtggcc 9961 gacgccctgg gtcgtgtgaa caaactggag gtgatccctc tggaggagct cggtcgtcca 10021 cgtgtggacg tggttgtcaa ctgctcgggt gtgttccgcg acctgttcgt gaaccagatg 10081 ctgctgttag accgtgcaat caagcttgca gcagagcagg acgagccaga tgagatgaac 10141 ttcgtgcgca agcacgccaa gcagcaggcc gccgagctag gtctgcaaag cctgcgcgac 10201 gcagcaaccc gtgtgttctc caacagctcg ggttcctact cgtccaacgt caacctggcg 10261 gtggagaaca gcagctggag cgacgagtcg cagctgcaag agatgtacct gaagcgcaag 10321 tcgtacgcat tcaactcgga ccgtccaggt gcaggtggtg agatgcagcg cgacgtgttc 10381 gagacggcaa tgaagaccgt ggacgtgacc ttccagaacc tggactcgtc cgagatctcg 10441 ctgaccgatg tgtcgcacta cttcgactct gacccaacga agctggttgc atcgcttcgt 10501 aacgatggtc gtacacctaa cgcatacatc gcagacacca ctactgctaa cgcgcaggtc 10561 cgcactcttg gtgagactgt gcgtctggac gcacgcacca agctgctcaa tcctaagtgg 10621 tacgaaggaa tgcttgcctc aggatacgaa ggagtgcgcg agatccagaa gcgcatgacc 10681 aacaccatgg gatggtctgc tacctcggga atggtggaca actgggtgta cgacgaggct 10741 aactcgacct tcatcgagga tgcggccatg gctgagcgcc tgatgaacac caaccctaac 10801 agcttccgca agctggtggc taccttcctg gaagccaacg gacgcggcta ctgggatgct 10861 aagcctgagc agctggaacg tctgcgtcag ctgtacatgg acgtggagga caagattgag 10921 ggagtcgaat aataataa //