BBa_K1080011 1 "Eye 2" ChlI2 2013-09-21T11:00:00Z 2016-10-20T07:08:00Z Chlamydomonas reinhardtii Long Description here false false _1389_ 7708 18463 9 It's complicated false Design Considerations here false Macquarie University annotation2525682 1 ChlI2 range2525682 1 25 1206 annotation2525681 1 RBS range2525681 1 6 16 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961224 1 -35 range1961224 1 137 142 BBa_K1080005 1 "CTH1" CTH1 2013-09-21T11:00:00Z 2016-10-20T07:03:00Z Chlamydomonas reinhardtii DSTGHGGTRE false false _1389_ 7708 18612 9 In stock false RETHYDYRHTE false Macquarie University annotation2525677 1 RBS range2525677 1 6 16 annotation2525678 1 CTH1 range2525678 1 25 1146 BBa_K1080000 1 "Eye 1" ChlI1 2013-09-21T11:00:00Z 2016-10-20T06:51:00Z Chlamydomonas reinhardtii hghghg false false _1389_ 7708 18612 9 In stock false jhjhjhj false Macquarie University annotation2525650 1 RBS range2525650 1 6 16 annotation2525651 1 ChlI1 range2525651 1 25 1113 BBa_K1640019 1 BBa_K1640019 ChlH 2015-09-09T11:00:00Z 2016-10-20T07:10:40Z n/a n/a false false _2057_ 7708 28565 9 false n/a false Meghan Cook annotation2474962 1 ChlH range2474962 1 20 4114 annotation2474961 1 RBS range2474961 1 1 11 BBa_K1080003 1 "The Gun" GUN4 2013-09-11T11:00:00Z 2016-10-20T06:59:15Z Chlamydomonas reinhardtii Magnesium chelate co-factor false false _1389_ 7708 18463 9 It's complicated true Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence false Macquarie University annotation2525657 1 GUN4 range2525657 1 25 675 annotation2525656 1 RBS range2525656 1 6 16 BBa_K1080002 1 "D" ChlD 2013-09-21T11:00:00Z 2016-10-20T06:52:59Z Chlamydomonas reinhardtii fsdsd false false _1389_ 7708 18612 9 It's complicated true dfsgdggfd false Macquarie University annotation2525653 1 ChlD range2525653 1 25 2154 annotation2525652 1 RBS range2525652 1 6 16 BBa_K1326008 1 BBa_K1326008 Plac + ChlI1 + ChlD + GUN4 2014-10-15T11:00:00Z 2015-05-08T01:09:54Z Chlamydomonas reinhardtii Composite part comprised of genes encoding Magnesium chelatase subunits false false _1701_ 0 23617 9 Not in stock true Codon optimisation false Leah Simmons component2427732 1 BBa_K1080002 component2427724 1 BBa_R0010 component2427733 1 BBa_K1080003 component2427731 1 BBa_K1080000 annotation2427724 1 BBa_R0010 range2427724 1 1 200 annotation2427732 1 BBa_K1080002 range2427732 1 1333 3486 annotation2427731 1 BBa_K1080000 range2427731 1 209 1324 annotation2427733 1 BBa_K1080003 range2427733 1 3495 4222 BBa_K1998000 1 BBa_K1998000 Mg-Chelatase Plasmid 2016-10-11T11:00:00Z 2016-10-17T01:47:37Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. This composite part is made up of various genes that form a part of the chlorophyll biosynthesis pathway. ChlI1 and ChlI2 forms a Mg-chelatase complex which catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. ChID forms an ATP dependent complex with ChlI1 and ChlI2 before acting of protoporphyrin. GUN4 is a tetrapyrrole-binding protein which controls the production of Mg-protoporphyrin IX found only in aerobic photsynthetic organisms. It is thought to participate in plastid-to-nucleus signalling by regulating Mg-protoporphyrin IX synthesis. Finally, ChlH is a chloroplast precursor which plays a role in the regulation of light in chlorophyll biosynthesis. CTH1 is a functional protein that is developed in the prescense of oxygen or copper. It catalyzes Mg-protoporphyrin IX monomethyl, which converts to divinyl protochlorophyllide in the presence of NADPH and O2. false false _2465_ 33907 33907 9 false Codon optimisation, order of parts in operon, illegal enzyme kinetics. false Shauna Winchester component2514140 1 BBa_K1080011 component2514142 1 BBa_R0010 component2514139 1 BBa_K1326008 component2514151 1 BBa_K1640019 component2514141 1 BBa_K1080005 annotation2514151 1 BBa_K1640019 range2514151 1 6819 10938 annotation2514142 1 BBa_R0010 range2514142 1 6611 6810 annotation2514140 1 BBa_K1080011 range2514140 1 4231 5442 annotation2514141 1 BBa_K1080005 range2514141 1 5451 6602 annotation2514139 1 BBa_K1326008 range2514139 1 1 4222 BBa_K1080000_sequence 1 agctttaagaaggagatatacataatggcagctactgaggtcaaggctgctgaaggccgtactgagaaggagctgggtcaggcacgtccaatctttccattcaccgccatcgttggtcaggatgagatgaagctggcgctgattctgaacgtgatcgatcctaagatcggtggtgtcatgatcatgggtgaccgtggtactggtaagtccactactattcgtgcactggcagatctgctgcctgaaatgcaggtggttgctaacgatccatttaactcggatccaactgatccagagctgatgagtgaagaagttcgtaaccgtgtcaaggcaggtgaacagctgccagtgtcttccaagaagattcctatggttgacctgccactgggtgctactgaagatcgtgtgtgcggtaccatcgatatcgagaaggcactgactgaaggtgttaaggcattcgagcctggtctgctggctaaggctaaccgtggtatcctgtacgtggatgaagtcaacctgctggatgatcatctggttgatgttctgctggactcggcagcatctggttggaacaccgtggagcgtgaaggtatctccatcagccacccagcccgcttcatcctggttggttcgggtaacccagaggaaggtgaactgcgtccacagctgctggatcgcttcggcatgcacgctcagatcggtaccgtcaaagatcctcgtctgcgtgtgcaaatcgtgtcgcagcgctcgaccttcgacgagaatccagccgcctttcgcaaggactacgaagctggtcagatggcactgacacagcgtatcgttgacgctcgtaagctgctgaagcaaggtgaagtcaactacgacttccgcgtcaagatcagccagatctgctcggacctgaacgtggacggcatccgtggtgatatcgtgactaaccgtgcagctaaggctctggctgcattcgaaggtcgtaccgaagtgactccagaagacatctaccgtgtcattccactgtgcctgcgtcaccgtctccgtaaagacccactggctgagatcgacgacggtgaccgtgtgcgtgagatcttcaagcaggtgttcggcatggagtaataa BBa_K1998000_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggcagctactgaggtcaaggctgctgaaggccgtactgagaaggagctgggtcaggcacgtccaatctttccattcaccgccatcgttggtcaggatgagatgaagctggcgctgattctgaacgtgatcgatcctaagatcggtggtgtcatgatcatgggtgaccgtggtactggtaagtccactactattcgtgcactggcagatctgctgcctgaaatgcaggtggttgctaacgatccatttaactcggatccaactgatccagagctgatgagtgaagaagttcgtaaccgtgtcaaggcaggtgaacagctgccagtgtcttccaagaagattcctatggttgacctgccactgggtgctactgaagatcgtgtgtgcggtaccatcgatatcgagaaggcactgactgaaggtgttaaggcattcgagcctggtctgctggctaaggctaaccgtggtatcctgtacgtggatgaagtcaacctgctggatgatcatctggttgatgttctgctggactcggcagcatctggttggaacaccgtggagcgtgaaggtatctccatcagccacccagcccgcttcatcctggttggttcgggtaacccagaggaaggtgaactgcgtccacagctgctggatcgcttcggcatgcacgctcagatcggtaccgtcaaagatcctcgtctgcgtgtgcaaatcgtgtcgcagcgctcgaccttcgacgagaatccagccgcctttcgcaaggactacgaagctggtcagatggcactgacacagcgtatcgttgacgctcgtaagctgctgaagcaaggtgaagtcaactacgacttccgcgtcaagatcagccagatctgctcggacctgaacgtggacggcatccgtggtgatatcgtgactaaccgtgcagctaaggctctggctgcattcgaaggtcgtaccgaagtgactccagaagacatctaccgtgtcattccactgtgcctgcgtcaccgtctccgtaaagacccactggctgagatcgacgacggtgaccgtgtgcgtgagatcttcaagcaggtgttcggcatggagtaataatactagagagctttaagaaggagatatacataatgcgcgccatgaaggtgtctgaggaggactccaagggcttcgatgcggatgtgtcgacccgcctggcccgctcgtaccctctggcggccgtggtgggccaggacaacatcaagcaggcgctgctgctgggcgccgtggacaccgggctgggcggcatcgccatcgccggtcgccgcggtaccgccaagtccatcatggctcgcggcctgcacgctctgctgccgcccattgaggtggtggagggcagcatctgcaacgccgaccccgaggacccccgctcctgggaggctggcctggctgagaagtatgcgggcggccctgtgaagaccaagatgcgctcggcgccgtttgtgcagatccctctgggtgtgactgaggaccgcttggtgggcactgtggacattgaggcgtccatgaaggagggcaagactgtgttccagcccggcctgctggctgaggcgcaccgcggcatcctgtacgtggacgagatcaacctgctggatgacggcattgccaacctgctgctgtccatcctgtcggacggagtcaacgtggtggagcgcgagggcatctccatcagccacccctgccggccgctgctgattgccacctacaaccccgaggagggccctctgcgtgagcacctgctggaccgcatcgccattggcctcagcgccgacgtccccagcaccagcgacgagcgcgtcaaggccattgacgcagccatccgcttccaggacaagccgcaggacactattgacgacaccgcggagctcaccgacgccctgcgcacctcggtcatcctggctcgcgagtacctgaaggacgtgaccatcgcgccggagcaggtgacctacattgtggaggaggcgcgccgcggcggagtccaggggcaccgcgcggagctgtacgcggtcaagtgtgccaaggcgtgtgcggctctggagggccgtgagcgtgtgaacaaggatgacctgcgccaggccgtgcagctggtcatcctgccgcgcgccaccatcctggaccagcccccgcccgagcaggagcagcccccgccgccgcccccgccccctcccccgccgccgccgcaggaccaaatggaggacgaggaccaggaggagaaggaggacgagaaggaggaggaggagaaggagaacgaggaccaggacgagcccgagatccctcaggagttcatgtttgagtccgagggcgtcatcatggacccctccatcctcatgttcgcgcagcagcagcagcgcgcgcagggccgctccggccgcgccaagacgctcatcttcagcgacgaccgcggccgctacatcaagcccatgctgcccaagggtgacaaggtcaagcgcctggcagtggacgccacgcttcgcgccgccgcgccctaccagaagattcgccggcagcaggccatcagcgagggcaaggtgcagcgcaaggtgtacgtggacaagccagacatgcgctccaagaagctggcccgcaaggccggtgcgctggtgatttttgttgtggacgcgtccggctccatggctctgaaccgcatgagcgccgccaagggcgcctgcatgcgcctgctggctgagtcgtacaccagccgcgaccaggtgtgcctcatccccttctacggcgacaaggccgaggtgctgctgccgccctccaagtccatcgccatggcccgccgccgcctggactcgctgccctgcggcggcggctcgccccttgcgcacggcctgtccacggcggtacgtgtgggcatgcaggccagccaggcgggcgaggtgggccgcgtcatgatggtgctcatcacggacggccgcgccaacgtcagcctggccaagtccaacgaggaccccgaggcgctcaagcccgacgcgcccaagcccaccgccgactcgctgaaggacgaggtgcgcgacatggccaagaaggccgcgtccgccggcatcaacgtgcttgtcattgacacggagaacaagttcgtgagcaccggctttgcggaggagatctccaaggcagcgcagggcaagtactactacctgcccaacgccagcgacgcagcaatcgcagcagcagcatccggtgcaatggcagcagctaagggaggatactagcaataatactagagagctttaagaaggagatatacataatggcgatgcgtgtcaccgtcgctgctggtaagctggactctgtctccctgttcggtggagacaccgccagcctgatgggtggttcacagactgtggagaagaagaagtctggtaaggaggctgtgatggaggtgcagctcagctccacagcaggcatcgactacaccgtccttcgtgaccaccttgccaacggtgagttccgtgaggcagaggacgagactcgtgctctgctgatcaagcttgctggtccagaagcagtcaagcgcaactgggtttacttcaccgaggtgaagaacatttccgtcaccgatttccagaccctggacaacctgtggaaggcgtccagcaacaacaagttcggctacagcgttcagaaggagatctgggtgcagaaccagaagcgctggcccaagttcttcaagcagattgactggacccagggcgagaacaacaactaccggaagtggcccatggagttcatctacagcatggacgccccccgcggccacctgcccctcaccaacgcgctccgagggacgcagctgttccaggcgatcatggagcacccagcgttcgagaagtccagcacggcaaagacgcttgatcagaaggcggcggaggccgccgggcggacacagtcgctgttctaagctctcagcagctcagcggctgatcctgccaggtgttgtatcagcggccgccttactagagagctttaagaaggagatatacataatgccatccacgaaagcagctaagaagccgaacttcccattcgtcaagattcaaggtcaagaggaaatgaagcttgcactgctgctgaacgttgttgatccaaacatcggtggtgtacttattatgggtgaccgtggtactgctaagtcggtcgcagtccgtgctctggtggatatgcttccagacattgacgtggttgaaggtgacgcattcaacagctctccaacagatcctaagttcatgggtccagacacactgcaacgtttccgtaacggcgagaagctgccaaccgttcgtatgcggacaccactggttgaactgcctctgggtgcaaccgaggatcgtatctgcggtacaatcgatatcgagaaggcactgacgcagggtatcaaggcatacgaacctggtctgctggcaaaggctaaccgtggtatcctgtatgtggacgaagtgaacctgctggatgatggtctggttgatgtcgtgctggactcgtcggctagcggtctgaacactgtggaacgtgaaggtgtgtctattgtgcatcctgcacgtttcatcatgattggttcaggtaacccccaggaaggtgaactgcgtccacagctgctggatcgtttcggcatgagcgtcaacgtggcaacgctgcaagacaccaagcaacgtacgcagctggtgctggatcgtcttgcgtacgaagctgaccctgacgcatttgtggactcgtgcaaggcagagcagacagcactcacggacaagctggaagcagcacgtcaacgtctgcgttccgtcaagatcagcgaagaactgcaaatcctgatctctgacatttgctctcgtctggatgttgatggtctgcgtggtgacattgttatcaaccgtgctgctaaggctcttgttgcattcgaaggtcgtaccgaagttaccacgaatgacgtggaacgtgtcatctcgggttgcctcaaccatcgtctgcgtaaggacccactggaccccattgacaacggcactaaggttgcaatcctgttcaagcgtatgactgatccagaaatcatgaagcgtgaagaagaagctaagaagaagcgcgaagaggctgctgccaaggctaaggctgaaggtaaggctgatcgtcctacgggtgctaaggctggtgcatgggctggcttgccacctcgtcggtaataataatactagagagctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagaagaaggagatatacatatgtgtaatgttgcgaccggtccccgtcccccaatgaccacttttaccggtggaaacaaaggcccggcgaaacagcaggttagcctggacctgcgtgatgatggtgcaggcatgtttacctctacaagccctgagatgcgccgtgttgtaccagatgatgttaaaggtcgcgtaaaagtcaaggtagtatatgtggtgctggaggcgcaatatcagagcgctatttccgcggcggtgaaaaatatcaacgcgaaaaattccaaggtatgtttcgaagtcgtgggctacctgctggaggaactccgcgaccagaaaaatttagacatgttaaaggaggatgtcgcgagcgctaacatttttatcggcagtttgatttttattgaagagctggcagaaaagatcgtggaggccgtcagccccctgcgtgagaaattggacgcttgcttgatcttcccctccatgccagctgttatgaagttaaacaaactgggcacgttttctatggcacagcttggtcaaagcaaaagtgttttcagcgaatttattaaatctgcccgtaaaaacaatgacaactttgaggagggtctgttaaaactggttcgcactttgcccaaagtgctgaagtacctgcctagcgataaagcgcaagatgcgaagaactttgttaatagtttgcagtattggctgggtggcaactctgataacttggaaaatctgttgttaaacaccgtttccaactacgttccggctctgaaaggcgttgatttttccgtggccgaaccgacggcctatccggatgttggtatctggcatccactggcatccggaatgtacgaagatctcaaagaatatctgaattggtatgatacccgtaaagatatggtttttgctaaagatgcccctgtgattggcttagtcctccagcgttctcatttagtcaccggtgacgagggccattattctggtgtggtggcagagctggagtcgcgcggcgctaaggttatcccagtttttgcgggtggtttagattttagcgcgccagtgaaaaaattcttttatgatcccttgggtagtgggcgcaccttcgtggataccgtcgtctcactgaccggtttcgcactggttggggggccggcgcgtcaagacgccccgaaagcaattgaagcgctgaaaaacctcaatgtgccgtatctggttagtctgccgctcgtatttcagaccacggaagaatggttagattcagagctgggtgtacatccagtgcaagtggcattgcaggtagccctgcctgaactggatggcgcgatggaaccgattgtttttgcgggtcgtgattctaacaccggcaaaagtcactcacttccagatcgtattgcgtccctgtgtgcccgcgcggtcaattgggcgaacctgcgtaagaaacgcaacgcggaaaaaaaattagcggtaaccgttttctcctttccgccggataagggtaacgtgggtaccgcggcctacttaaatgtatttggctcaatttaccgcgtactcaaaaaccttcagcgcgaagggtatgatgttggtgcccttccgcctagtgaggaagatcttatccaaagtgtcctgactcagaaagaggctaaattcaattcaaccgacctgcacattgcatacaaaatgaaagttgacgagtatcagaagctttgcccgtatgcagaagcgctggaagaaaactggggtaagcctccaggcacacttaacaccaatggccaagagctgctggtatatgggcgtcaatacggcaacgtgttcattggtgtgcagcctacctttggttatgagggcgatccgatgcgtctgcttttttcaaagtccgcgtccccacatcacggtttcgctgcttattacacgtttctggaaaaaattttcaaagcagatgctgtgctccacttcgggacccatgggtccctggaatttatgcccggtaaacaggtcggcatgtctggtgtatgttatccggattcactgattggcactattccaaacctctattattatgcagccaacaatccaagtgaggcaaccattgccaaacgtcgcagctacgcaaatacgatctcttatctgaccccaccggcagagaatgcaggtctgtacaaggggttaaaagaactgaaagaacttattagtagctatcaaggaatgcgcgaaagcggtcgcgcggagcagatctgtgcgacgattattgaaacggctaaactgtgtaacttggatcgcgacgtgacgctcccggatgccgacgccaaagatctgaccatggacatgcgtgattctgtggtgggccaagtttaccgtaagctgatggagatcgaatcacgcctgctgccgtgcggtttgcacgtggttggctgtcctccgactgctgaagaggccgtcgctactttagtcaacattgcagaactggaccgcccagacaacaacccaccgattaaagggatgccggggattcttgcgcgtgctattggccgcgatattgaaagcatttactccggtaacaacaagggcgtcctggctgacgttgaccagctgcaacgcatcaccgaggcctcccgtacctgcgtgcgtgagttcgtgaaggaccgcaccggtctgaacggtcgtatcggcaccaactggatcaccaacctgctcaagttcaccggtttctacgtggacccctgggtgcgtggtctgcaaaacggtgagttcgccagcgcaaaccgtgaggagctgatcaccctgttcaactacctggagttctgcctgacccaggtggtcaaggacaacgagctgggtgcactggtagaggcactgaacggtcagtacgtcgagccaggtccaggtggtgaccccatccgcaaccccaacgtgctgcccaccggtaagaacatccacgcactggaccctcagtcgattcccactcaggcagcactgaagagcgcacgtctggtggtggaccgtctgctggaccgtgagcgcgacaacaacggcggcaagtaccccgagaccatcgcgctggtgctgtggggcactgacaacatcaagacctacggcgagtcgctggcccaggtcatgatgatggtcggtgtcaagcccgtggccgacgccctgggtcgtgtgaacaaactggaggtgatccctctggaggagctcggtcgtccacgtgtggacgtggttgtcaactgctcgggtgtgttccgcgacctgttcgtgaaccagatgctgctgttagaccgtgcaatcaagcttgcagcagagcaggacgagccagatgagatgaacttcgtgcgcaagcacgccaagcagcaggccgccgagctaggtctgcaaagcctgcgcgacgcagcaacccgtgtgttctccaacagctcgggttcctactcgtccaacgtcaacctggcggtggagaacagcagctggagcgacgagtcgcagctgcaagagatgtacctgaagcgcaagtcgtacgcattcaactcggaccgtccaggtgcaggtggtgagatgcagcgcgacgtgttcgagacggcaatgaagaccgtggacgtgaccttccagaacctggactcgtccgagatctcgctgaccgatgtgtcgcactacttcgactctgacccaacgaagctggttgcatcgcttcgtaacgatggtcgtacacctaacgcatacatcgcagacaccactactgctaacgcgcaggtccgcactcttggtgagactgtgcgtctggacgcacgcaccaagctgctcaatcctaagtggtacgaaggaatgcttgcctcaggatacgaaggagtgcgcgagatccagaagcgcatgaccaacaccatgggatggtctgctacctcgggaatggtggacaactgggtgtacgacgaggctaactcgaccttcatcgaggatgcggccatggctgagcgcctgatgaacaccaaccctaacagcttccgcaagctggtggctaccttcctggaagccaacggacgcggctactgggatgctaagcctgagcagctggaacgtctgcgtcagctgtacatggacgtggaggacaagattgagggagtcgaataataataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1326008_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggcagctactgaggtcaaggctgctgaaggccgtactgagaaggagctgggtcaggcacgtccaatctttccattcaccgccatcgttggtcaggatgagatgaagctggcgctgattctgaacgtgatcgatcctaagatcggtggtgtcatgatcatgggtgaccgtggtactggtaagtccactactattcgtgcactggcagatctgctgcctgaaatgcaggtggttgctaacgatccatttaactcggatccaactgatccagagctgatgagtgaagaagttcgtaaccgtgtcaaggcaggtgaacagctgccagtgtcttccaagaagattcctatggttgacctgccactgggtgctactgaagatcgtgtgtgcggtaccatcgatatcgagaaggcactgactgaaggtgttaaggcattcgagcctggtctgctggctaaggctaaccgtggtatcctgtacgtggatgaagtcaacctgctggatgatcatctggttgatgttctgctggactcggcagcatctggttggaacaccgtggagcgtgaaggtatctccatcagccacccagcccgcttcatcctggttggttcgggtaacccagaggaaggtgaactgcgtccacagctgctggatcgcttcggcatgcacgctcagatcggtaccgtcaaagatcctcgtctgcgtgtgcaaatcgtgtcgcagcgctcgaccttcgacgagaatccagccgcctttcgcaaggactacgaagctggtcagatggcactgacacagcgtatcgttgacgctcgtaagctgctgaagcaaggtgaagtcaactacgacttccgcgtcaagatcagccagatctgctcggacctgaacgtggacggcatccgtggtgatatcgtgactaaccgtgcagctaaggctctggctgcattcgaaggtcgtaccgaagtgactccagaagacatctaccgtgtcattccactgtgcctgcgtcaccgtctccgtaaagacccactggctgagatcgacgacggtgaccgtgtgcgtgagatcttcaagcaggtgttcggcatggagtaataatactagagagctttaagaaggagatatacataatgcgcgccatgaaggtgtctgaggaggactccaagggcttcgatgcggatgtgtcgacccgcctggcccgctcgtaccctctggcggccgtggtgggccaggacaacatcaagcaggcgctgctgctgggcgccgtggacaccgggctgggcggcatcgccatcgccggtcgccgcggtaccgccaagtccatcatggctcgcggcctgcacgctctgctgccgcccattgaggtggtggagggcagcatctgcaacgccgaccccgaggacccccgctcctgggaggctggcctggctgagaagtatgcgggcggccctgtgaagaccaagatgcgctcggcgccgtttgtgcagatccctctgggtgtgactgaggaccgcttggtgggcactgtggacattgaggcgtccatgaaggagggcaagactgtgttccagcccggcctgctggctgaggcgcaccgcggcatcctgtacgtggacgagatcaacctgctggatgacggcattgccaacctgctgctgtccatcctgtcggacggagtcaacgtggtggagcgcgagggcatctccatcagccacccctgccggccgctgctgattgccacctacaaccccgaggagggccctctgcgtgagcacctgctggaccgcatcgccattggcctcagcgccgacgtccccagcaccagcgacgagcgcgtcaaggccattgacgcagccatccgcttccaggacaagccgcaggacactattgacgacaccgcggagctcaccgacgccctgcgcacctcggtcatcctggctcgcgagtacctgaaggacgtgaccatcgcgccggagcaggtgacctacattgtggaggaggcgcgccgcggcggagtccaggggcaccgcgcggagctgtacgcggtcaagtgtgccaaggcgtgtgcggctctggagggccgtgagcgtgtgaacaaggatgacctgcgccaggccgtgcagctggtcatcctgccgcgcgccaccatcctggaccagcccccgcccgagcaggagcagcccccgccgccgcccccgccccctcccccgccgccgccgcaggaccaaatggaggacgaggaccaggaggagaaggaggacgagaaggaggaggaggagaaggagaacgaggaccaggacgagcccgagatccctcaggagttcatgtttgagtccgagggcgtcatcatggacccctccatcctcatgttcgcgcagcagcagcagcgcgcgcagggccgctccggccgcgccaagacgctcatcttcagcgacgaccgcggccgctacatcaagcccatgctgcccaagggtgacaaggtcaagcgcctggcagtggacgccacgcttcgcgccgccgcgccctaccagaagattcgccggcagcaggccatcagcgagggcaaggtgcagcgcaaggtgtacgtggacaagccagacatgcgctccaagaagctggcccgcaaggccggtgcgctggtgatttttgttgtggacgcgtccggctccatggctctgaaccgcatgagcgccgccaagggcgcctgcatgcgcctgctggctgagtcgtacaccagccgcgaccaggtgtgcctcatccccttctacggcgacaaggccgaggtgctgctgccgccctccaagtccatcgccatggcccgccgccgcctggactcgctgccctgcggcggcggctcgccccttgcgcacggcctgtccacggcggtacgtgtgggcatgcaggccagccaggcgggcgaggtgggccgcgtcatgatggtgctcatcacggacggccgcgccaacgtcagcctggccaagtccaacgaggaccccgaggcgctcaagcccgacgcgcccaagcccaccgccgactcgctgaaggacgaggtgcgcgacatggccaagaaggccgcgtccgccggcatcaacgtgcttgtcattgacacggagaacaagttcgtgagcaccggctttgcggaggagatctccaaggcagcgcagggcaagtactactacctgcccaacgccagcgacgcagcaatcgcagcagcagcatccggtgcaatggcagcagctaagggaggatactagcaataatactagagagctttaagaaggagatatacataatggcgatgcgtgtcaccgtcgctgctggtaagctggactctgtctccctgttcggtggagacaccgccagcctgatgggtggttcacagactgtggagaagaagaagtctggtaaggaggctgtgatggaggtgcagctcagctccacagcaggcatcgactacaccgtccttcgtgaccaccttgccaacggtgagttccgtgaggcagaggacgagactcgtgctctgctgatcaagcttgctggtccagaagcagtcaagcgcaactgggtttacttcaccgaggtgaagaacatttccgtcaccgatttccagaccctggacaacctgtggaaggcgtccagcaacaacaagttcggctacagcgttcagaaggagatctgggtgcagaaccagaagcgctggcccaagttcttcaagcagattgactggacccagggcgagaacaacaactaccggaagtggcccatggagttcatctacagcatggacgccccccgcggccacctgcccctcaccaacgcgctccgagggacgcagctgttccaggcgatcatggagcacccagcgttcgagaagtccagcacggcaaagacgcttgatcagaaggcggcggaggccgccgggcggacacagtcgctgttctaagctctcagcagctcagcggctgatcctgccaggtgttgtatcagcggccgcct BBa_K1080003_sequence 1 agctttaagaaggagatatacataatggcgatgcgtgtcaccgtcgctgctggtaagctggactctgtctccctgttcggtggagacaccgccagcctgatgggtggttcacagactgtggagaagaagaagtctggtaaggaggctgtgatggaggtgcagctcagctccacagcaggcatcgactacaccgtccttcgtgaccaccttgccaacggtgagttccgtgaggcagaggacgagactcgtgctctgctgatcaagcttgctggtccagaagcagtcaagcgcaactgggtttacttcaccgaggtgaagaacatttccgtcaccgatttccagaccctggacaacctgtggaaggcgtccagcaacaacaagttcggctacagcgttcagaaggagatctgggtgcagaaccagaagcgctggcccaagttcttcaagcagattgactggacccagggcgagaacaacaactaccggaagtggcccatggagttcatctacagcatggacgccccccgcggccacctgcccctcaccaacgcgctccgagggacgcagctgttccaggcgatcatggagcacccagcgttcgagaagtccagcacggcaaagacgcttgatcagaaggcggcggaggccgccgggcggacacagtcgctgttctaagctctcagcagctcagcggctgatcctgccaggtgttgtatcagcggccgcct BBa_K1080005_sequence 1 agctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataa BBa_K1080011_sequence 1 agctttaagaaggagatatacataatgccatccacgaaagcagctaagaagccgaacttcccattcgtcaagattcaaggtcaagaggaaatgaagcttgcactgctgctgaacgttgttgatccaaacatcggtggtgtacttattatgggtgaccgtggtactgctaagtcggtcgcagtccgtgctctggtggatatgcttccagacattgacgtggttgaaggtgacgcattcaacagctctccaacagatcctaagttcatgggtccagacacactgcaacgtttccgtaacggcgagaagctgccaaccgttcgtatgcggacaccactggttgaactgcctctgggtgcaaccgaggatcgtatctgcggtacaatcgatatcgagaaggcactgacgcagggtatcaaggcatacgaacctggtctgctggcaaaggctaaccgtggtatcctgtatgtggacgaagtgaacctgctggatgatggtctggttgatgtcgtgctggactcgtcggctagcggtctgaacactgtggaacgtgaaggtgtgtctattgtgcatcctgcacgtttcatcatgattggttcaggtaacccccaggaaggtgaactgcgtccacagctgctggatcgtttcggcatgagcgtcaacgtggcaacgctgcaagacaccaagcaacgtacgcagctggtgctggatcgtcttgcgtacgaagctgaccctgacgcatttgtggactcgtgcaaggcagagcagacagcactcacggacaagctggaagcagcacgtcaacgtctgcgttccgtcaagatcagcgaagaactgcaaatcctgatctctgacatttgctctcgtctggatgttgatggtctgcgtggtgacattgttatcaaccgtgctgctaaggctcttgttgcattcgaaggtcgtaccgaagttaccacgaatgacgtggaacgtgtcatctcgggttgcctcaaccatcgtctgcgtaaggacccactggaccccattgacaacggcactaaggttgcaatcctgttcaagcgtatgactgatccagaaatcatgaagcgtgaagaagaagctaagaagaagcgcgaagaggctgctgccaaggctaaggctgaaggtaaggctgatcgtcctacgggtgctaaggctggtgcatgggctggcttgccacctcgtcggtaataataa BBa_K1080002_sequence 1 agctttaagaaggagatatacataatgcgcgccatgaaggtgtctgaggaggactccaagggcttcgatgcggatgtgtcgacccgcctggcccgctcgtaccctctggcggccgtggtgggccaggacaacatcaagcaggcgctgctgctgggcgccgtggacaccgggctgggcggcatcgccatcgccggtcgccgcggtaccgccaagtccatcatggctcgcggcctgcacgctctgctgccgcccattgaggtggtggagggcagcatctgcaacgccgaccccgaggacccccgctcctgggaggctggcctggctgagaagtatgcgggcggccctgtgaagaccaagatgcgctcggcgccgtttgtgcagatccctctgggtgtgactgaggaccgcttggtgggcactgtggacattgaggcgtccatgaaggagggcaagactgtgttccagcccggcctgctggctgaggcgcaccgcggcatcctgtacgtggacgagatcaacctgctggatgacggcattgccaacctgctgctgtccatcctgtcggacggagtcaacgtggtggagcgcgagggcatctccatcagccacccctgccggccgctgctgattgccacctacaaccccgaggagggccctctgcgtgagcacctgctggaccgcatcgccattggcctcagcgccgacgtccccagcaccagcgacgagcgcgtcaaggccattgacgcagccatccgcttccaggacaagccgcaggacactattgacgacaccgcggagctcaccgacgccctgcgcacctcggtcatcctggctcgcgagtacctgaaggacgtgaccatcgcgccggagcaggtgacctacattgtggaggaggcgcgccgcggcggagtccaggggcaccgcgcggagctgtacgcggtcaagtgtgccaaggcgtgtgcggctctggagggccgtgagcgtgtgaacaaggatgacctgcgccaggccgtgcagctggtcatcctgccgcgcgccaccatcctggaccagcccccgcccgagcaggagcagcccccgccgccgcccccgccccctcccccgccgccgccgcaggaccaaatggaggacgaggaccaggaggagaaggaggacgagaaggaggaggaggagaaggagaacgaggaccaggacgagcccgagatccctcaggagttcatgtttgagtccgagggcgtcatcatggacccctccatcctcatgttcgcgcagcagcagcagcgcgcgcagggccgctccggccgcgccaagacgctcatcttcagcgacgaccgcggccgctacatcaagcccatgctgcccaagggtgacaaggtcaagcgcctggcagtggacgccacgcttcgcgccgccgcgccctaccagaagattcgccggcagcaggccatcagcgagggcaaggtgcagcgcaaggtgtacgtggacaagccagacatgcgctccaagaagctggcccgcaaggccggtgcgctggtgatttttgttgtggacgcgtccggctccatggctctgaaccgcatgagcgccgccaagggcgcctgcatgcgcctgctggctgagtcgtacaccagccgcgaccaggtgtgcctcatccccttctacggcgacaaggccgaggtgctgctgccgccctccaagtccatcgccatggcccgccgccgcctggactcgctgccctgcggcggcggctcgccccttgcgcacggcctgtccacggcggtacgtgtgggcatgcaggccagccaggcgggcgaggtgggccgcgtcatgatggtgctcatcacggacggccgcgccaacgtcagcctggccaagtccaacgaggaccccgaggcgctcaagcccgacgcgcccaagcccaccgccgactcgctgaaggacgaggtgcgcgacatggccaagaaggccgcgtccgccggcatcaacgtgcttgtcattgacacggagaacaagttcgtgagcaccggctttgcggaggagatctccaaggcagcgcagggcaagtactactacctgcccaacgccagcgacgcagcaatcgcagcagcagcatccggtgcaatggcagcagctaagggaggatactagcaataa BBa_K1640019_sequence 1 agaagaaggagatatacatatgtgtaatgttgcgaccggtccccgtcccccaatgaccacttttaccggtggaaacaaaggcccggcgaaacagcaggttagcctggacctgcgtgatgatggtgcaggcatgtttacctctacaagccctgagatgcgccgtgttgtaccagatgatgttaaaggtcgcgtaaaagtcaaggtagtatatgtggtgctggaggcgcaatatcagagcgctatttccgcggcggtgaaaaatatcaacgcgaaaaattccaaggtatgtttcgaagtcgtgggctacctgctggaggaactccgcgaccagaaaaatttagacatgttaaaggaggatgtcgcgagcgctaacatttttatcggcagtttgatttttattgaagagctggcagaaaagatcgtggaggccgtcagccccctgcgtgagaaattggacgcttgcttgatcttcccctccatgccagctgttatgaagttaaacaaactgggcacgttttctatggcacagcttggtcaaagcaaaagtgttttcagcgaatttattaaatctgcccgtaaaaacaatgacaactttgaggagggtctgttaaaactggttcgcactttgcccaaagtgctgaagtacctgcctagcgataaagcgcaagatgcgaagaactttgttaatagtttgcagtattggctgggtggcaactctgataacttggaaaatctgttgttaaacaccgtttccaactacgttccggctctgaaaggcgttgatttttccgtggccgaaccgacggcctatccggatgttggtatctggcatccactggcatccggaatgtacgaagatctcaaagaatatctgaattggtatgatacccgtaaagatatggtttttgctaaagatgcccctgtgattggcttagtcctccagcgttctcatttagtcaccggtgacgagggccattattctggtgtggtggcagagctggagtcgcgcggcgctaaggttatcccagtttttgcgggtggtttagattttagcgcgccagtgaaaaaattcttttatgatcccttgggtagtgggcgcaccttcgtggataccgtcgtctcactgaccggtttcgcactggttggggggccggcgcgtcaagacgccccgaaagcaattgaagcgctgaaaaacctcaatgtgccgtatctggttagtctgccgctcgtatttcagaccacggaagaatggttagattcagagctgggtgtacatccagtgcaagtggcattgcaggtagccctgcctgaactggatggcgcgatggaaccgattgtttttgcgggtcgtgattctaacaccggcaaaagtcactcacttccagatcgtattgcgtccctgtgtgcccgcgcggtcaattgggcgaacctgcgtaagaaacgcaacgcggaaaaaaaattagcggtaaccgttttctcctttccgccggataagggtaacgtgggtaccgcggcctacttaaatgtatttggctcaatttaccgcgtactcaaaaaccttcagcgcgaagggtatgatgttggtgcccttccgcctagtgaggaagatcttatccaaagtgtcctgactcagaaagaggctaaattcaattcaaccgacctgcacattgcatacaaaatgaaagttgacgagtatcagaagctttgcccgtatgcagaagcgctggaagaaaactggggtaagcctccaggcacacttaacaccaatggccaagagctgctggtatatgggcgtcaatacggcaacgtgttcattggtgtgcagcctacctttggttatgagggcgatccgatgcgtctgcttttttcaaagtccgcgtccccacatcacggtttcgctgcttattacacgtttctggaaaaaattttcaaagcagatgctgtgctccacttcgggacccatgggtccctggaatttatgcccggtaaacaggtcggcatgtctggtgtatgttatccggattcactgattggcactattccaaacctctattattatgcagccaacaatccaagtgaggcaaccattgccaaacgtcgcagctacgcaaatacgatctcttatctgaccccaccggcagagaatgcaggtctgtacaaggggttaaaagaactgaaagaacttattagtagctatcaaggaatgcgcgaaagcggtcgcgcggagcagatctgtgcgacgattattgaaacggctaaactgtgtaacttggatcgcgacgtgacgctcccggatgccgacgccaaagatctgaccatggacatgcgtgattctgtggtgggccaagtttaccgtaagctgatggagatcgaatcacgcctgctgccgtgcggtttgcacgtggttggctgtcctccgactgctgaagaggccgtcgctactttagtcaacattgcagaactggaccgcccagacaacaacccaccgattaaagggatgccggggattcttgcgcgtgctattggccgcgatattgaaagcatttactccggtaacaacaagggcgtcctggctgacgttgaccagctgcaacgcatcaccgaggcctcccgtacctgcgtgcgtgagttcgtgaaggaccgcaccggtctgaacggtcgtatcggcaccaactggatcaccaacctgctcaagttcaccggtttctacgtggacccctgggtgcgtggtctgcaaaacggtgagttcgccagcgcaaaccgtgaggagctgatcaccctgttcaactacctggagttctgcctgacccaggtggtcaaggacaacgagctgggtgcactggtagaggcactgaacggtcagtacgtcgagccaggtccaggtggtgaccccatccgcaaccccaacgtgctgcccaccggtaagaacatccacgcactggaccctcagtcgattcccactcaggcagcactgaagagcgcacgtctggtggtggaccgtctgctggaccgtgagcgcgacaacaacggcggcaagtaccccgagaccatcgcgctggtgctgtggggcactgacaacatcaagacctacggcgagtcgctggcccaggtcatgatgatggtcggtgtcaagcccgtggccgacgccctgggtcgtgtgaacaaactggaggtgatccctctggaggagctcggtcgtccacgtgtggacgtggttgtcaactgctcgggtgtgttccgcgacctgttcgtgaaccagatgctgctgttagaccgtgcaatcaagcttgcagcagagcaggacgagccagatgagatgaacttcgtgcgcaagcacgccaagcagcaggccgccgagctaggtctgcaaagcctgcgcgacgcagcaacccgtgtgttctccaacagctcgggttcctactcgtccaacgtcaacctggcggtggagaacagcagctggagcgacgagtcgcagctgcaagagatgtacctgaagcgcaagtcgtacgcattcaactcggaccgtccaggtgcaggtggtgagatgcagcgcgacgtgttcgagacggcaatgaagaccgtggacgtgaccttccagaacctggactcgtccgagatctcgctgaccgatgtgtcgcactacttcgactctgacccaacgaagctggttgcatcgcttcgtaacgatggtcgtacacctaacgcatacatcgcagacaccactactgctaacgcgcaggtccgcactcttggtgagactgtgcgtctggacgcacgcaccaagctgctcaatcctaagtggtacgaaggaatgcttgcctcaggatacgaaggagtgcgcgagatccagaagcgcatgaccaacaccatgggatggtctgctacctcgggaatggtggacaactgggtgtacgacgaggctaactcgaccttcatcgaggatgcggccatggctgagcgcctgatgaacaccaaccctaacagcttccgcaagctggtggctaccttcctggaagccaacggacgcggctactgggatgctaagcctgagcagctggaacgtctgcgtcagctgtacatggacgtggaggacaagattgagggagtcgaataataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z