BBa_K1998000
type: ComponentDefinition
persistent identity: BBa K1998000
display id: BBa_K1998000
version: 1
was generated by: iGEM to SBOL conversion
description: Mg-Chelatase Plasmid
created: 2016-10-11T11:00:00Z
modified: 2016-10-17T01:47:37Z
mutable provenance: The genes were sourced from Chlamydomonas rienhardtii and synthesised.
owned by: james
owned by: myers
mutable description: This composite part is made up of various genes that form a part of the chlorophyll biosynthesis pathway. ChlI1 and ChlI2 forms a Mg-chelatase complex which catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. ChID forms an ATP dependent complex with ChlI1 and ChlI2 before acting of protoporphyrin. GUN4 is a tetrapyrrole-binding protein which controls the production of Mg-protoporphyrin IX found only in aerobic photsynthetic organisms. It is thought to participate in plastid-to-nucleus signalling by regulating Mg-protoporphyrin IX synthesis. Finally, ChlH is a chloroplast precursor which plays a role in the regulation of light in chlorophyll biosynthesis. CTH1 is a functional protein that is developed in the prescense of oxygen or copper. It catalyzes Mg-protoporphyrin IX monomethyl, which converts to divinyl protochlorophyllide in the presence of NADPH and O2.
discontinued: false
dominant: false
experience: experience/None
group u list: _2465_
m user id: 33907
owner id: 33907
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Codon optimisation, order of parts in operon, illegal enzyme kinetics.
star: false
creator: Shauna Winchester
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1326008
component: BBa_K1640019
component: BBa_R0010
component: BBa_K1080005
component: BBa_K1080011
sequence annotation: BBa_K1326008
sequence annotation: BBa_K1080011
sequence annotation: BBa_K1080005
sequence annotation: BBa_R0010
sequence annotation: BBa_K1640019
sequence: https://synbiohub.org/public/igem/BBa_K1998000_sequence/1

BBa_K1326008
type: Component
persistent identity: component2514139
display id: component2514139
version: 1
top level: BBa_K1998000
definition: BBa_K1326008
access: public

BBa_K1640019
type: Component
persistent identity: component2514151
display id: component2514151
version: 1
top level: BBa_K1998000
definition: BBa_K1640019
access: public

BBa_R0010
type: Component
persistent identity: component2514142
display id: component2514142
version: 1
top level: BBa_K1998000
definition: LacI
access: public

BBa_K1080005
type: Component
persistent identity: component2514141
display id: component2514141
version: 1
top level: BBa_K1998000
definition: "CTH1"
access: public

BBa_K1080011
type: Component
persistent identity: component2514140
display id: component2514140
version: 1
top level: BBa_K1998000
definition: "Eye 2"
access: public

BBa_K1326008
type: SequenceAnnotation
persistent identity: annotation2514139
display id: annotation2514139
version: 1
top level: BBa_K1998000
location: https://synbiohub.org/public/igem/BBa_K1998000/annotation2514139/range2514139/1
component: BBa_K1326008

https://synbiohub.org/public/igem/BBa_K1998000/annotation2514139/range2514139/1
type: Range
persistent identity: range2514139
display id: range2514139
version: 1
top level: BBa_K1998000
direction: direction/reverse
start: 1
end: 4222
orientation: inline

BBa_K1080011
type: SequenceAnnotation
persistent identity: annotation2514140
display id: annotation2514140
version: 1
top level: BBa_K1998000
location: https://synbiohub.org/public/igem/BBa_K1998000/annotation2514140/range2514140/1
component: BBa_K1080011

https://synbiohub.org/public/igem/BBa_K1998000/annotation2514140/range2514140/1
type: Range
persistent identity: range2514140
display id: range2514140
version: 1
top level: BBa_K1998000
direction: direction/reverse
start: 4231
end: 5442
orientation: inline

BBa_K1080005
type: SequenceAnnotation
persistent identity: annotation2514141
display id: annotation2514141
version: 1
top level: BBa_K1998000
location: https://synbiohub.org/public/igem/BBa_K1998000/annotation2514141/range2514141/1
component: BBa_K1080005

https://synbiohub.org/public/igem/BBa_K1998000/annotation2514141/range2514141/1
type: Range
persistent identity: range2514141
display id: range2514141
version: 1
top level: BBa_K1998000
direction: direction/reverse
start: 5451
end: 6602
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2514142
display id: annotation2514142
version: 1
top level: BBa_K1998000
location: https://synbiohub.org/public/igem/BBa_K1998000/annotation2514142/range2514142/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1998000/annotation2514142/range2514142/1
type: Range
persistent identity: range2514142
display id: range2514142
version: 1
top level: BBa_K1998000
direction: direction/forward
start: 6611
end: 6810
orientation: inline

BBa_K1640019
type: SequenceAnnotation
persistent identity: annotation2514151
display id: annotation2514151
version: 1
top level: BBa_K1998000
location: https://synbiohub.org/public/igem/BBa_K1998000/annotation2514151/range2514151/1
component: BBa_K1640019

https://synbiohub.org/public/igem/BBa_K1998000/annotation2514151/range2514151/1
type: Range
persistent identity: range2514151
display id: range2514151
version: 1
top level: BBa_K1998000
direction: direction/reverse
start: 6819
end: 10938
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K1326008
type: ComponentDefinition
persistent identity: BBa K1326008
display id: BBa_K1326008
version: 1
was generated by: iGEM to SBOL conversion
description: Plac + ChlI1 + ChlD + GUN4
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:09:54Z
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
mutable description: Composite part comprised of genes encoding Magnesium chelatase subunits
discontinued: false
dominant: false
experience: experience/None
group u list: _1701_
m user id: 0
owner id: 23617
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Codon optimisation
star: false
creator: Leah Simmons
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1080000
component: BBa_R0010
component: BBa_K1080003
component: BBa_K1080002
sequence annotation: BBa_R0010
sequence annotation: BBa_K1080000
sequence annotation: BBa_K1080002
sequence annotation: BBa_K1080003
sequence: https://synbiohub.org/public/igem/BBa_K1326008_sequence/1

BBa_K1080000
type: Component
persistent identity: component2427731
display id: component2427731
version: 1
top level: BBa_K1326008
definition: "Eye 1"
access: public

BBa_R0010
type: Component
persistent identity: component2427724
display id: component2427724
version: 1
top level: BBa_K1326008
definition: LacI
access: public

BBa_K1080003
type: Component
persistent identity: component2427733
display id: component2427733
version: 1
top level: BBa_K1326008
definition: "The Gun"
access: public

BBa_K1080002
type: Component
persistent identity: component2427732
display id: component2427732
version: 1
top level: BBa_K1326008
definition: "D"
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2427724
display id: annotation2427724
version: 1
top level: BBa_K1326008
location: https://synbiohub.org/public/igem/BBa_K1326008/annotation2427724/range2427724/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1326008/annotation2427724/range2427724/1
type: Range
persistent identity: range2427724
display id: range2427724
version: 1
top level: BBa_K1326008
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K1080000
type: SequenceAnnotation
persistent identity: annotation2427731
display id: annotation2427731
version: 1
top level: BBa_K1326008
location: https://synbiohub.org/public/igem/BBa_K1326008/annotation2427731/range2427731/1
component: BBa_K1080000

https://synbiohub.org/public/igem/BBa_K1326008/annotation2427731/range2427731/1
type: Range
persistent identity: range2427731
display id: range2427731
version: 1
top level: BBa_K1326008
direction: direction/reverse
start: 209
end: 1324
orientation: inline

BBa_K1080002
type: SequenceAnnotation
persistent identity: annotation2427732
display id: annotation2427732
version: 1
top level: BBa_K1326008
location: https://synbiohub.org/public/igem/BBa_K1326008/annotation2427732/range2427732/1
component: BBa_K1080002

https://synbiohub.org/public/igem/BBa_K1326008/annotation2427732/range2427732/1
type: Range
persistent identity: range2427732
display id: range2427732
version: 1
top level: BBa_K1326008
direction: direction/reverse
start: 1333
end: 3486
orientation: inline

BBa_K1080003
type: SequenceAnnotation
persistent identity: annotation2427733
display id: annotation2427733
version: 1
top level: BBa_K1326008
location: https://synbiohub.org/public/igem/BBa_K1326008/annotation2427733/range2427733/1
component: BBa_K1080003

https://synbiohub.org/public/igem/BBa_K1326008/annotation2427733/range2427733/1
type: Range
persistent identity: range2427733
display id: range2427733
version: 1
top level: BBa_K1326008
direction: direction/reverse
start: 3495
end: 4222
orientation: inline

"CTH1"
type: ComponentDefinition
persistent identity: BBa K1080005
display id: BBa_K1080005
version: 1
was generated by: iGEM to SBOL conversion
description: CTH1
created: 2013-09-21T11:00:00Z
modified: 2016-10-20T07:03:00Z
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
mutable description: DSTGHGGTRE
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 7708
owner id: 18612
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: RETHYDYRHTE
star: false
creator: Macquarie University
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: CTH1
sequence: https://synbiohub.org/public/igem/BBa_K1080005_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2525677
display id: annotation2525677
version: 1
top level: "CTH1"
location: https://synbiohub.org/public/igem/BBa_K1080005/annotation2525677/range2525677/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1080005/annotation2525677/range2525677/1
type: Range
persistent identity: range2525677
display id: range2525677
version: 1
top level: "CTH1"
direction: direction/forward
start: 6
end: 16
orientation: inline

CTH1
type: SequenceAnnotation
persistent identity: annotation2525678
display id: annotation2525678
version: 1
top level: "CTH1"
location: https://synbiohub.org/public/igem/BBa_K1080005/annotation2525678/range2525678/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1080005/annotation2525678/range2525678/1
type: Range
persistent identity: range2525678
display id: range2525678
version: 1
top level: "CTH1"
direction: direction/forward
start: 25
end: 1146
orientation: inline

"Eye 2"
type: ComponentDefinition
persistent identity: BBa K1080011
display id: BBa_K1080011
version: 1
was generated by: iGEM to SBOL conversion
description: ChlI2
created: 2013-09-21T11:00:00Z
modified: 2016-10-20T07:08:00Z
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
mutable description: Long Description here
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 7708
owner id: 18463
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Design Considerations here
star: false
creator: Macquarie University
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: RBS
sequence annotation: ChlI2
sequence: https://synbiohub.org/public/igem/BBa_K1080011_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2525681
display id: annotation2525681
version: 1
top level: "Eye 2"
location: https://synbiohub.org/public/igem/BBa_K1080011/annotation2525681/range2525681/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1080011/annotation2525681/range2525681/1
type: Range
persistent identity: range2525681
display id: range2525681
version: 1
top level: "Eye 2"
direction: direction/forward
start: 6
end: 16
orientation: inline

ChlI2
type: SequenceAnnotation
persistent identity: annotation2525682
display id: annotation2525682
version: 1
top level: "Eye 2"
location: https://synbiohub.org/public/igem/BBa_K1080011/annotation2525682/range2525682/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1080011/annotation2525682/range2525682/1
type: Range
persistent identity: range2525682
display id: range2525682
version: 1
top level: "Eye 2"
direction: direction/forward
start: 25
end: 1206
orientation: inline

BBa_K1640019
type: ComponentDefinition
persistent identity: BBa K1640019
display id: BBa_K1640019
version: 1
was generated by: iGEM to SBOL conversion
description: ChlH
created: 2015-09-09T11:00:00Z
modified: 2016-10-20T07:10:40Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: n/a
discontinued: false
dominant: false
group u list: _2057_
m user id: 7708
owner id: 28565
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: n/a
star: false
creator: Meghan Cook
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: RBS
sequence annotation: ChlH
sequence: https://synbiohub.org/public/igem/BBa_K1640019_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2474961
display id: annotation2474961
version: 1
top level: BBa_K1640019
location: https://synbiohub.org/public/igem/BBa_K1640019/annotation2474961/range2474961/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1640019/annotation2474961/range2474961/1
type: Range
persistent identity: range2474961
display id: range2474961
version: 1
top level: BBa_K1640019
direction: direction/forward
start: 1
end: 11
orientation: inline

ChlH
type: SequenceAnnotation
persistent identity: annotation2474962
display id: annotation2474962
version: 1
top level: BBa_K1640019
location: https://synbiohub.org/public/igem/BBa_K1640019/annotation2474962/range2474962/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1640019/annotation2474962/range2474962/1
type: Range
persistent identity: range2474962
display id: range2474962
version: 1
top level: BBa_K1640019
direction: direction/forward
start: 20
end: 4114
orientation: inline

"Eye 1"
type: ComponentDefinition
persistent identity: BBa K1080000
display id: BBa_K1080000
version: 1
was generated by: iGEM to SBOL conversion
description: ChlI1
created: 2013-09-21T11:00:00Z
modified: 2016-10-20T06:51:00Z
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
mutable description: hghghg
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 7708
owner id: 18612
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: jhjhjhj
star: false
creator: Macquarie University
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: RBS
sequence annotation: ChlI1
sequence: https://synbiohub.org/public/igem/BBa_K1080000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2525650
display id: annotation2525650
version: 1
top level: "Eye 1"
location: https://synbiohub.org/public/igem/BBa_K1080000/annotation2525650/range2525650/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1080000/annotation2525650/range2525650/1
type: Range
persistent identity: range2525650
display id: range2525650
version: 1
top level: "Eye 1"
direction: direction/forward
start: 6
end: 16
orientation: inline

ChlI1
type: SequenceAnnotation
persistent identity: annotation2525651
display id: annotation2525651
version: 1
top level: "Eye 1"
location: https://synbiohub.org/public/igem/BBa_K1080000/annotation2525651/range2525651/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1080000/annotation2525651/range2525651/1
type: Range
persistent identity: range2525651
display id: range2525651
version: 1
top level: "Eye 1"
direction: direction/forward
start: 25
end: 1113
orientation: inline

"The Gun"
type: ComponentDefinition
persistent identity: BBa K1080003
display id: BBa_K1080003
version: 1
was generated by: iGEM to SBOL conversion
description: GUN4
created: 2013-09-11T11:00:00Z
modified: 2016-10-20T06:59:15Z
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
mutable description: Magnesium chelate co-factor
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 7708
owner id: 18463
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence
star: false
creator: Macquarie University
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: GUN4
sequence: https://synbiohub.org/public/igem/BBa_K1080003_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2525656
display id: annotation2525656
version: 1
top level: "The Gun"
location: https://synbiohub.org/public/igem/BBa_K1080003/annotation2525656/range2525656/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1080003/annotation2525656/range2525656/1
type: Range
persistent identity: range2525656
display id: range2525656
version: 1
top level: "The Gun"
direction: direction/forward
start: 6
end: 16
orientation: inline

GUN4
type: SequenceAnnotation
persistent identity: annotation2525657
display id: annotation2525657
version: 1
top level: "The Gun"
location: https://synbiohub.org/public/igem/BBa_K1080003/annotation2525657/range2525657/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1080003/annotation2525657/range2525657/1
type: Range
persistent identity: range2525657
display id: range2525657
version: 1
top level: "The Gun"
direction: direction/forward
start: 25
end: 675
orientation: inline

"D"
type: ComponentDefinition
persistent identity: BBa K1080002
display id: BBa_K1080002
version: 1
was generated by: iGEM to SBOL conversion
description: ChlD
created: 2013-09-21T11:00:00Z
modified: 2016-10-20T06:52:59Z
mutable provenance: Chlamydomonas reinhardtii
owned by: james
owned by: myers
mutable description: fsdsd
discontinued: false
dominant: false
experience: experience/None
group u list: _1389_
m user id: 7708
owner id: 18612
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: dfsgdggfd
star: false
creator: Macquarie University
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: RBS
sequence annotation: ChlD
sequence: https://synbiohub.org/public/igem/BBa_K1080002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2525652
display id: annotation2525652
version: 1
top level: "D"
location: https://synbiohub.org/public/igem/BBa_K1080002/annotation2525652/range2525652/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1080002/annotation2525652/range2525652/1
type: Range
persistent identity: range2525652
display id: range2525652
version: 1
top level: "D"
direction: direction/forward
start: 6
end: 16
orientation: inline

ChlD
type: SequenceAnnotation
persistent identity: annotation2525653
display id: annotation2525653
version: 1
top level: "D"
location: https://synbiohub.org/public/igem/BBa_K1080002/annotation2525653/range2525653/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1080002/annotation2525653/range2525653/1
type: Range
persistent identity: range2525653
display id: range2525653
version: 1
top level: "D"
direction: direction/forward
start: 25
end: 2154
orientation: inline

https://synbiohub.org/public/igem/BBa_K1998000_sequence/1
type: Sequence
persistent identity: BBa K1998000 sequence
display id: BBa_K1998000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggcagctactgaggtcaaggctgctgaaggccgtactgagaaggagctgggtcaggcacgtccaatctttccattcaccgccatcgttggtcaggatgagatgaagctggcgctgattctgaacgtgatcgatcctaagatcggtggtgtcatgatcatgggtgaccgtggtactggtaagtccactactattcgtgcactggcagatctgctgcctgaaatgcaggtggttgctaacgatccatttaactcggatccaactgatccagagctgatgagtgaagaagttcgtaaccgtgtcaaggcaggtgaacagctgccagtgtcttccaagaagattcctatggttgacctgccactgggtgctactgaagatcgtgtgtgcggtaccatcgatatcgagaaggcactgactgaaggtgttaaggcattcgagcctggtctgctggctaaggctaaccgtggtatcctgtacgtggatgaagtcaacctgctggatgatcatctggttgatgttctgctggactcggcagcatctggttggaacaccgtggagcgtgaaggtatctccatcagccacccagcccgcttcatcctggttggttcgggtaacccagaggaaggtgaactgcgtccacagctgctggatcgcttcggcatgcacgctcagatcggtaccgtcaaagatcctcgtctgcgtgtgcaaatcgtgtcgcagcgctcgaccttcgacgagaatccagccgcctttcgcaaggactacgaagctggtcagatggcactgacacagcgtatcgttgacgctcgtaagctgctgaagcaaggtgaagtcaactacgacttccgcgtcaagatcagccagatctgctcggacctgaacgtggacggcatccgtggtgatatcgtgactaaccgtgcagctaaggctctggctgcattcgaaggtcgtaccgaagtgactccagaagacatctaccgtgtcattccactgtgcctgcgtcaccgtctccgtaaagacccactggctgagatcgacgacggtgaccgtgtgcgtgagatcttcaagcaggtgttcggcatggagtaataatactagagagctttaagaaggagatatacataatgcgcgccatgaaggtgtctgaggaggactccaagggcttcgatgcggatgtgtcgacccgcctggcccgctcgtaccctctggcggccgtggtgggccaggacaacatcaagcaggcgctgctgctgggcgccgtggacaccgggctgggcggcatcgccatcgccggtcgccgcggtaccgccaagtccatcatggctcgcggcctgcacgctctgctgccgcccattgaggtggtggagggcagcatctgcaacgccgaccccgaggacccccgctcctgggaggctggcctggctgagaagtatgcgggcggccctgtgaagaccaagatgcgctcggcgccgtttgtgcagatccctctgggtgtgactgaggaccgcttggtgggcactgtggacattgaggcgtccatgaaggagggcaagactgtgttccagcccggcctgctggctgaggcgcaccgcggcatcctgtacgtggacgagatcaacctgctggatgacggcattgccaacctgctgctgtccatcctgtcggacggagtcaacgtggtggagcgcgagggcatctccatcagccacccctgccggccgctgctgattgccacctacaaccccgaggagggccctctgcgtgagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1326008_sequence/1
type: Sequence
persistent identity: BBa K1326008 sequence
display id: BBa_K1326008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggcagctactgaggtcaaggctgctgaaggccgtactgagaaggagctgggtcaggcacgtccaatctttccattcaccgccatcgttggtcaggatgagatgaagctggcgctgattctgaacgtgatcgatcctaagatcggtggtgtcatgatcatgggtgaccgtggtactggtaagtccactactattcgtgcactggcagatctgctgcctgaaatgcaggtggttgctaacgatccatttaactcggatccaactgatccagagctgatgagtgaagaagttcgtaaccgtgtcaaggcaggtgaacagctgccagtgtcttccaagaagattcctatggttgacctgccactgggtgctactgaagatcgtgtgtgcggtaccatcgatatcgagaaggcactgactgaaggtgttaaggcattcgagcctggtctgctggctaaggctaaccgtggtatcctgtacgtggatgaagtcaacctgctggatgatcatctggttgatgttctgctggactcggcagcatctggttggaacaccgtggagcgtgaaggtatctccatcagccacccagcccgcttcatcctggttggttcgggtaacccagaggaaggtgaactgcgtccacagctgctggatcgcttcggcatgcacgctcagatcggtaccgtcaaagatcctcgtctgcgtgtgcaaatcgtgtcgcagcgctcgaccttcgacgagaatccagccgcctttcgcaaggactacgaagctggtcagatggcactgacacagcgtatcgttgacgctcgtaagctgctgaagcaaggtgaagtcaactacgacttccgcgtcaagatcagccagatctgctcggacctgaacgtggacggcatccgtggtgatatcgtgactaaccgtgcagctaaggctctggctgcattcgaaggtcgtaccgaagtgactccagaagacatctaccgtgtcattccactgtgcctgcgtcaccgtctccgtaaagacccactggctgagatcgacgacggtgaccgtgtgcgtgagatcttcaagcaggtgttcggcatggagtaataatactagagagctttaagaaggagatatacataatgcgcgccatgaaggtgtctgaggaggactccaagggcttcgatgcggatgtgtcgacccgcctggcccgctcgtaccctctggcggccgtggtgggccaggacaacatcaagcaggcgctgctgctgggcgccgtggacaccgggctgggcggcatcgccatcgccggtcgccgcggtaccgccaagtccatcatggctcgcggcctgcacgctctgctgccgcccattgaggtggtggagggcagcatctgcaacgccgaccccgaggacccccgctcctgggaggctggcctggctgagaagtatgcgggcggccctgtgaagaccaagatgcgctcggcgccgtttgtgcagatccctctgggtgtgactgaggaccgcttggtgggcactgtggacattgaggcgtccatgaaggagggcaagactgtgttccagcccggcctgctggctgaggcgcaccgcggcatcctgtacgtggacgagatcaacctgctggatgacggcattgccaacctgctgctgtccatcctgtcggacggagtcaacgtggtggagcgcgagggcatctccatcagccacccctgccggccgctgctgattgccacctacaaccccgaggagggccctctgcgtgagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1080005_sequence/1
type: Sequence
persistent identity: BBa K1080005 sequence
display id: BBa_K1080005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctttaagaaggagatatacataatggtggctgctaccgctgctccccaggaggttgagggcttcaaggttatgcgtgacggcatcaaggttgcttccgatgagaccctgctcactcctcgcttctacaccaccgacttcgatgagatggagcgtctgttcagcctggagctgaacaagaacatggacatggaggagttcgaggcgatgctgaacgagttcaagctggactacaaccagcgtcacttcgtccgcaacgagaccttcaaggaggcagcggagaagattcagggtcccacccgcaagatcttcattgagttcctggagcgctcgtgcactgctgagttctcgggtttcctgctctacaaggagctgggtcgtcgtctgaaggccacgaaccctgtggtcgcggagatcttcaccctgatgtcccgtgacgaggcacgtcacgctggcttcctgaacaaggccatgtccgacttcaacctggctctggacctgggtttcctgaccaagaaccgcaagtacaccttcttcaagcccaagttcatcttctacgccacttacctgtcggagaagattggctactggcgttacatttctatctaccgtcacctgcaacgcaacccagacaaccagctgtatccgctgttcgagtacttcgagaactggtgccaggacgagaaccgtcacggtgacttcttcaccgcagtgctgaaggcacgtccggagatggtcaacgactgggctgccaagctgtggtcgcgcttcttctgcctgtcggtgtacatcaccatgtacctgaacgaccaccagcgtgacgcattctacagctctcttggactgaacaccacacagttcaaccagcacgtgatcatcgagaccaacaagtctactgagcgtatcttccccgctgtgccggatgtggagaaccccgagttcttccgtcgtatggacctgctggtcaagtacaacgcacagctggtgaacattggaagcatgaacctgccaagtccaatcaaggctatcatgaaggcaccaattctggagcgcatggtcgctgaggtgttccaggtattcatcatgacacctaaggagtctggctcgtacgacctggatgccaacaagaccgctctggtctactaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1080011_sequence/1
type: Sequence
persistent identity: BBa K1080011 sequence
display id: BBa_K1080011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctttaagaaggagatatacataatgccatccacgaaagcagctaagaagccgaacttcccattcgtcaagattcaaggtcaagaggaaatgaagcttgcactgctgctgaacgttgttgatccaaacatcggtggtgtacttattatgggtgaccgtggtactgctaagtcggtcgcagtccgtgctctggtggatatgcttccagacattgacgtggttgaaggtgacgcattcaacagctctccaacagatcctaagttcatgggtccagacacactgcaacgtttccgtaacggcgagaagctgccaaccgttcgtatgcggacaccactggttgaactgcctctgggtgcaaccgaggatcgtatctgcggtacaatcgatatcgagaaggcactgacgcagggtatcaaggcatacgaacctggtctgctggcaaaggctaaccgtggtatcctgtatgtggacgaagtgaacctgctggatgatggtctggttgatgtcgtgctggactcgtcggctagcggtctgaacactgtggaacgtgaaggtgtgtctattgtgcatcctgcacgtttcatcatgattggttcaggtaacccccaggaaggtgaactgcgtccacagctgctggatcgtttcggcatgagcgtcaacgtggcaacgctgcaagacaccaagcaacgtacgcagctggtgctggatcgtcttgcgtacgaagctgaccctgacgcatttgtggactcgtgcaaggcagagcagacagcactcacggacaagctggaagcagcacgtcaacgtctgcgttccgtcaagatcagcgaagaactgcaaatcctgatctctgacatttgctctcgtctggatgttgatggtctgcgtggtgacattgttatcaaccgtgctgctaaggctcttgttgcattcgaaggtcgtaccgaagttaccacgaatgacgtggaacgtgtcatctcgggttgcctcaaccatcgtctgcgtaaggacccactggaccccattgacaacggcactaaggttgcaatcctgttcaagcgtatgactgatccagaaatcatgaagcgtgaagaagaagctaagaagaagcgcgaagaggctgctgccaaggctaaggctgaaggtaaggctgatcgtcctacgggtgctaaggctggtgcatgggctggcttgccacctcgtcggtaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1640019_sequence/1
type: Sequence
persistent identity: BBa K1640019 sequence
display id: BBa_K1640019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaagaaggagatatacatatgtgtaatgttgcgaccggtccccgtcccccaatgaccacttttaccggtggaaacaaaggcccggcgaaacagcaggttagcctggacctgcgtgatgatggtgcaggcatgtttacctctacaagccctgagatgcgccgtgttgtaccagatgatgttaaaggtcgcgtaaaagtcaaggtagtatatgtggtgctggaggcgcaatatcagagcgctatttccgcggcggtgaaaaatatcaacgcgaaaaattccaaggtatgtttcgaagtcgtgggctacctgctggaggaactccgcgaccagaaaaatttagacatgttaaaggaggatgtcgcgagcgctaacatttttatcggcagtttgatttttattgaagagctggcagaaaagatcgtggaggccgtcagccccctgcgtgagaaattggacgcttgcttgatcttcccctccatgccagctgttatgaagttaaacaaactgggcacgttttctatggcacagcttggtcaaagcaaaagtgttttcagcgaatttattaaatctgcccgtaaaaacaatgacaactttgaggagggtctgttaaaactggttcgcactttgcccaaagtgctgaagtacctgcctagcgataaagcgcaagatgcgaagaactttgttaatagtttgcagtattggctgggtggcaactctgataacttggaaaatctgttgttaaacaccgtttccaactacgttccggctctgaaaggcgttgatttttccgtggccgaaccgacggcctatccggatgttggtatctggcatccactggcatccggaatgtacgaagatctcaaagaatatctgaattggtatgatacccgtaaagatatggtttttgctaaagatgcccctgtgattggcttagtcctccagcgttctcatttagtcaccggtgacgagggccattattctggtgtggtggcagagctggagtcgcgcggcgctaaggttatcccagtttttgcgggtggtttagattttagcgcgccagtgaaaaaattcttttatgatcccttgggtagtgggcgcaccttcgtggataccgtcgtctcactgaccggtttcgcactggttggggggccggcgcgtcaagacgccccgaaagcaattgaagcgctgaaaaacctcaatgtgccgtatctggttagtctgccgctcgtatttcagaccacggaagaatggttagattcagagctgggtgtacatccagtgcaagtggcattgcaggtagccctgcctgaactggatggcgcgatggaaccgattgtttttgcgggtcgtgattctaacaccggcaaaagtcactcacttccagatcgtattgcgtccctgtgtgcccgcgcggtcaattgggcgaacctgcgtaagaaacgcaacgcggaaaaaaaattagcggtaaccgttttctcctttccgccggataagggtaacgtgggtaccgcggcctacttaaatgtatttggctcaatttaccgcgtactcaaaaaccttcagcgcgaagggtatgatgttggtgcccttccgcctagtgaggaagatcttatccaaagtgtcctgactcagaaagaggctaaattcaattcaaccgacctgcacattgcatacaaaatgaaagttgacgagtatcagaagctttgcccgtatgcagaagcgctggaagaaaactggggtaagcctccaggcacacttaacaccaatggccaagagctgctggtatatgggcgtcaatacggcaacgtgttcattggtgtgcagcctacctttggttatgagggcgatccgatgcgtctgcttttttcaaagtccgcgtccccacatcacggtttcgctgcttattacacgtttctggaaaaaattttcaaagcagatgctgtgctccacttcgggacccatgggtccctggaatttatgcccggtaaacaggtcggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1080000_sequence/1
type: Sequence
persistent identity: BBa K1080000 sequence
display id: BBa_K1080000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctttaagaaggagatatacataatggcagctactgaggtcaaggctgctgaaggccgtactgagaaggagctgggtcaggcacgtccaatctttccattcaccgccatcgttggtcaggatgagatgaagctggcgctgattctgaacgtgatcgatcctaagatcggtggtgtcatgatcatgggtgaccgtggtactggtaagtccactactattcgtgcactggcagatctgctgcctgaaatgcaggtggttgctaacgatccatttaactcggatccaactgatccagagctgatgagtgaagaagttcgtaaccgtgtcaaggcaggtgaacagctgccagtgtcttccaagaagattcctatggttgacctgccactgggtgctactgaagatcgtgtgtgcggtaccatcgatatcgagaaggcactgactgaaggtgttaaggcattcgagcctggtctgctggctaaggctaaccgtggtatcctgtacgtggatgaagtcaacctgctggatgatcatctggttgatgttctgctggactcggcagcatctggttggaacaccgtggagcgtgaaggtatctccatcagccacccagcccgcttcatcctggttggttcgggtaacccagaggaaggtgaactgcgtccacagctgctggatcgcttcggcatgcacgctcagatcggtaccgtcaaagatcctcgtctgcgtgtgcaaatcgtgtcgcagcgctcgaccttcgacgagaatccagccgcctttcgcaaggactacgaagctggtcagatggcactgacacagcgtatcgttgacgctcgtaagctgctgaagcaaggtgaagtcaactacgacttccgcgtcaagatcagccagatctgctcggacctgaacgtggacggcatccgtggtgatatcgtgactaaccgtgcagctaaggctctggctgcattcgaaggtcgtaccgaagtgactccagaagacatctaccgtgtcattccactgtgcctgcgtcaccgtctccgtaaagacccactggctgagatcgacgacggtgaccgtgtgcgtgagatcttcaagcaggtgttcggcatggagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1080003_sequence/1
type: Sequence
persistent identity: BBa K1080003 sequence
display id: BBa_K1080003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctttaagaaggagatatacataatggcgatgcgtgtcaccgtcgctgctggtaagctggactctgtctccctgttcggtggagacaccgccagcctgatgggtggttcacagactgtggagaagaagaagtctggtaaggaggctgtgatggaggtgcagctcagctccacagcaggcatcgactacaccgtccttcgtgaccaccttgccaacggtgagttccgtgaggcagaggacgagactcgtgctctgctgatcaagcttgctggtccagaagcagtcaagcgcaactgggtttacttcaccgaggtgaagaacatttccgtcaccgatttccagaccctggacaacctgtggaaggcgtccagcaacaacaagttcggctacagcgttcagaaggagatctgggtgcagaaccagaagcgctggcccaagttcttcaagcagattgactggacccagggcgagaacaacaactaccggaagtggcccatggagttcatctacagcatggacgccccccgcggccacctgcccctcaccaacgcgctccgagggacgcagctgttccaggcgatcatggagcacccagcgttcgagaagtccagcacggcaaagacgcttgatcagaaggcggcggaggccgccgggcggacacagtcgctgttctaagctctcagcagctcagcggctgatcctgccaggtgttgtatcagcggccgcct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1080002_sequence/1
type: Sequence
persistent identity: BBa K1080002 sequence
display id: BBa_K1080002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctttaagaaggagatatacataatgcgcgccatgaaggtgtctgaggaggactccaagggcttcgatgcggatgtgtcgacccgcctggcccgctcgtaccctctggcggccgtggtgggccaggacaacatcaagcaggcgctgctgctgggcgccgtggacaccgggctgggcggcatcgccatcgccggtcgccgcggtaccgccaagtccatcatggctcgcggcctgcacgctctgctgccgcccattgaggtggtggagggcagcatctgcaacgccgaccccgaggacccccgctcctgggaggctggcctggctgagaagtatgcgggcggccctgtgaagaccaagatgcgctcggcgccgtttgtgcagatccctctgggtgtgactgaggaccgcttggtgggcactgtggacattgaggcgtccatgaaggagggcaagactgtgttccagcccggcctgctggctgaggcgcaccgcggcatcctgtacgtggacgagatcaacctgctggatgacggcattgccaacctgctgctgtccatcctgtcggacggagtcaacgtggtggagcgcgagggcatctccatcagccacccctgccggccgctgctgattgccacctacaaccccgaggagggccctctgcgtgagcacctgctggaccgcatcgccattggcctcagcgccgacgtccccagcaccagcgacgagcgcgtcaaggccattgacgcagccatccgcttccaggacaagccgcaggacactattgacgacaccgcggagctcaccgacgccctgcgcacctcggtcatcctggctcgcgagtacctgaaggacgtgaccatcgcgccggagcaggtgacctacattgtggaggaggcgcgccgcggcggagtccaggggcaccgcgcggagctgtacgcggtcaagtgtgccaaggcgtgtgcggctctggagggccgtgagcgtgtgaacaaggatgacctgcgccaggccgtgcagctggtcatcctgccgcgcgccaccatcctggaccagcccccgcccgagcaggagcagcccccgccgccgcccccgccccctcccccgccgccgccgcaggaccaaatggaggacgaggaccaggaggagaaggaggacgagaaggaggaggaggagaaggagaacgaggaccaggacgagcccgagatccctcaggagttcatgtttgagtccgagggcgtcatcatggacccctccatcctcatgttcgcgcagcagcagcagcgcgcgcagggccgctccggccgcgccaagacgctcatcttcagcgacgaccgcggccgctacatcaagcccatgctgcccaagggtgacaaggtcaagcgcctggcagtggacgccacgcttcgcgccgccgcgccctaccagaagattcgccggcagcaggccatcagcgagggcaaggtgcagcgcaaggtgtacgtggacaagccagacatgcgctccaagaagctggcccgcaaggccggtgcgctggtgatttttgttgtggacgcgtccggctccatggctctgaaccgcatgagcgccgccaagggcgcctgcatgcgcctgctggctgagtcgtacaccagccgcgaccaggtgtgcctcatccccttctacggcgacaaggccgaggtgctgctgccgccctccaagtccatcgccatggcccgccgccgcctggactcgctgccctgcggcggcggctcgccccttgcgcacggcctgtccacggcggtacgtgtgggcatgcaggccagccaggcgggcgaggtgggccgcgtcatgatggtgctcatcacggacggccgcgccaacgtcagcctggccaagtccaacgaggaccccgaggcgctcaagcccgacgcgcccaagcccaccgccgactcgctgaaggacgaggtgcgcgacatggccaagaaggccgcgtccgccggcatcaacgtgcttgtcattga…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z