BBa_K1998001 1 BBa_K1998001 YCF54 - ChlM 2016-10-12T11:00:00Z 2016-10-18T05:05:59Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. This composite part is made up of YCF54 and ChlM. ChlM - Mg protoporphyrin IX S-adenosyl methionine O-methyl transferase false false _2465_ 33907 33907 9 false Codon optimisation, order of parts in operon, illegal enzyme kinetics false Shauna Winchester component2516665 1 BBa_K1080010 component2516668 1 BBa_K1640018 component2516658 1 BBa_R0010 annotation2516668 1 BBa_K1640018 range2516668 1 688 1549 annotation2516665 1 BBa_K1080010 range2516665 1 209 679 annotation2516658 1 BBa_R0010 range2516658 1 1 200 BBa_K1640018 1 BBa_K1640018 CHlM 2015-09-09T11:00:00Z 2016-10-20T07:15:09Z n/a n/a false false _2057_ 7708 28565 9 true n/a false Meghan Cook annotation2474919 1 ChlM range2474919 1 20 862 annotation2474918 1 RBS range2474918 1 1 11 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961224 1 -35 range1961224 1 137 142 BBa_K1080010 1 "YCF" YCF54 2013-09-21T11:00:00Z 2016-10-20T07:15:17Z Chlamydomonas reinhardtii Long Description here false false _1389_ 7708 18463 9 In stock false Design Considerations here false Macquarie University annotation2525684 1 25 range2525684 1 25 471 annotation2525683 1 RBS range2525683 1 6 16 BBa_K1080010_sequence 1 agctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1998001_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaatactagagagaagaaggagatatacatatggcttctgagattgctcaaactgccgatgttggttcgctgaccttcgctgttggtggagttggtgctgttgtaggtctgggtgcactgcttgtcgctactgatcctcagaagcgccgctcggaacagatgaagtcattcgatggtgatgagaaggaagcagtgaaggactacttcaacactgctggcttcgaacgttggcgtaagatctacggtgagacagacgaagtgaacaaggttcagcttgacatccgtactggccatgcacagacagttgacaaggttctgcgttgggtagacgaagaaggtagcgtacaaggtatcacagttgcagactgcggttgcggtacaggtagcctggctatcccgctggcactgcgtggcgcagctgtcagcgcatctgacatctctgctgcaatggcgtctgaagctgagcagcgttaccaacaagctgttgcagcaggtcaaggtaaggcacctaaggttgcacctaagttcgaagctcttgatctggaatctgtcaaaggtaagtacgacacggtcacttgcctggacgtgatgattcactacccccaggacaaggtggacgccatgatcacgcacctggcaggtctgtctgatcgtcgtctcatcatctcgttcgcacctaagacgctgtcgtacagcatcctcaagcgcattggtgaactgttccctggtccaagcaaggccacacgtgcatacctgcaccgtgaagaagacgtggaagctgctctcaagcgtgcaggtttcaaggtcaccaagcgtgaaatgaccgcaaccagcttctacttctcgcgcctgctggaagctatccgtgaataa BBa_K1640018_sequence 1 agaagaaggagatatacatatggcttctgagattgctcaaactgccgatgttggttcgctgaccttcgctgttggtggagttggtgctgttgtaggtctgggtgcactgcttgtcgctactgatcctcagaagcgccgctcggaacagatgaagtcattcgatggtgatgagaaggaagcagtgaaggactacttcaacactgctggcttcgaacgttggcgtaagatctacggtgagacagacgaagtgaacaaggttcagcttgacatccgtactggccatgcacagacagttgacaaggttctgcgttgggtagacgaagaaggtagcgtacaaggtatcacagttgcagactgcggttgcggtacaggtagcctggctatcccgctggcactgcgtggcgcagctgtcagcgcatctgacatctctgctgcaatggcgtctgaagctgagcagcgttaccaacaagctgttgcagcaggtcaaggtaaggcacctaaggttgcacctaagttcgaagctcttgatctggaatctgtcaaaggtaagtacgacacggtcacttgcctggacgtgatgattcactacccccaggacaaggtggacgccatgatcacgcacctggcaggtctgtctgatcgtcgtctcatcatctcgttcgcacctaagacgctgtcgtacagcatcctcaagcgcattggtgaactgttccctggtccaagcaaggccacacgtgcatacctgcaccgtgaagaagacgtggaagctgctctcaagcgtgcaggtttcaaggtcaccaagcgtgaaatgaccgcaaccagcttctacttctcgcgcctgctggaagctatccgtgaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z