BBa_K1998004 1 BBa_K1998004 psbMZHWK 2016-10-11T11:00:00Z 2016-10-17T10:11:16Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. This part is composed of the psbM, psbZ, psbH, psbW and psbK genes. The psbM protein subunit is positioned at the monomer-monomer interface. The psbZ protein controls the interaction of Photosystem II cores with the light-harvesting antenna. The psbH protein is required for stability and assembly of the photosystem II complex. The psbW protein stabilizes dimeric photosytem II. The psbK protein is also required for stability and assembly of Photosystem II. false false _2465_ 33907 33907 9 false Considerations taken into account: psbZ had no stop codon Genes were codon optimised for bacteria using optimiser tool false Shauna Winchester component2495357 1 BBa_K1640021 component2495351 1 BBa_K1640008 annotation2495357 1 BBa_K1640021 range2495357 1 497 1057 annotation2495351 1 BBa_K1640008 range2495351 1 1 488 BBa_K1640008 1 BBa_K1640008 psbMZH 2015-09-09T11:00:00Z 2015-09-18T08:53:22Z N/a N/a false false _2057_ 28565 28565 9 true N/a false Meghan Cook annotation2448249 1 rbs range2448249 1 308 320 annotation2448248 1 psbZ range2448248 1 176 307 annotation2448247 1 rbs range2448247 1 163 175 annotation2448245 1 rbs range2448245 1 41 57 annotation2448244 1 Plac range2448244 1 1 40 annotation2448250 1 psbH range2448250 1 321 488 annotation2448246 1 psbM range2448246 1 58 162 BBa_K1998006 1 BBa_K1998006 psbOPQR 2016-10-11T11:00:00Z 2016-10-12T05:42:34Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. This part is composed of the psbO, psbP, psbQ and psbR genes. he psbO gene that comprises this part ecodes a manganese stabilizing protein. The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster. The psbQ protein is an oxygen enhancer protein. The psbR protein is an important link in the Photosystem II core complex for stable assembly of the Oxygen Evolving Complex. false false _2465_ 33907 33907 9 false A premature stop codon was found in pubs and sequence was adjusted. false Shauna Winchester component2495383 1 BBa_K1640022 component2495380 1 BBa_K1640023 component2495389 1 BBa_K1640012 annotation2495383 1 BBa_K1640022 range2495383 1 864 1539 annotation2495389 1 BBa_K1640012 range2495389 1 1548 2588 annotation2495380 1 BBa_K1640023 range2495380 1 1 855 BBa_K1640021 1 BBa_K1640021 psbWK 2015-09-17T11:00:00Z 2015-09-18T02:01:50Z n/a n/a false false _2057_ 16353 28565 9 false n/a false Meghan Cook annotation2469502 1 RBS range2469502 1 279 291 annotation2469474 1 RBS range2469474 1 1 17 annotation2469501 1 psbW range2469501 1 18 278 annotation2469503 1 psbK range2469503 1 292 432 annotation2469504 1 terminator range2469504 1 433 561 BBa_K1640023 1 BBa_K1640023 psbO 2015-09-17T11:00:00Z 2015-09-18T12:14:16Z n/a n/a false false _2057_ 16353 28565 9 false n/a false Meghan Cook annotation2469602 1 psbO range2469602 1 58 855 annotation2469600 1 Plac range2469600 1 1 40 annotation2469601 1 RBS range2469601 1 41 57 BBa_K1640022 1 BBa_K1640022 psbP 2015-09-17T11:00:00Z 2015-09-17T11:58:33Z n.a n/a false false _2057_ 16353 28565 9 false n/a false Meghan Cook annotation2469509 1 psbP range2469509 1 20 676 annotation2469508 1 RBS range2469508 1 1 19 BBa_K1998007 1 BBa_K1998007 psbMZHWK-OPQR 2016-10-11T11:00:00Z 2016-10-12T05:47:20Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. This part is composed of two operons in the photosystem II pathway. The psbM, psbZ, psbH, psbW and psbK genes. The psbM protein subunit is positioned at the monomer-monomer interface. The psbZ protein controls the interaction of Photosystem II cores with the light-harvesting antenna. The psbH protein is required for stability and assembly of the photosystem II complex. The psbW protein stabilizes dimeric photosytem II. The psbK protein is also required for stability and assembly of Photosystem II. The second operon is composed of the psbO, psbP, psbQ and psbR genes. The psbO gene that comprises this part ecodes a manganese stabilizing protein. The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster. The psbQ protein is an oxygen enhancer protein. The psbR protein is an important link in the Photosystem II core complex for stable assembly of the Oxygen Evolving Complex. false false _2465_ 33907 33907 9 false Genes were codon optimised for bacteria using optimiser tool. Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence. false Shauna Winchester component2495404 1 BBa_K1998004 component2495418 1 BBa_K1998006 annotation2495404 1 BBa_K1998004 range2495404 1 1 1057 annotation2495418 1 BBa_K1998006 range2495418 1 1066 3653 BBa_K1640012 1 BBa_K1640012 psbQR 2015-09-09T11:00:00Z 2015-09-10T01:02:41Z n/a n/a false false _2057_ 16353 28565 9 false n/a false Meghan Cook annotation2448236 1 rbs range2448236 1 554 570 annotation2448238 1 double terminator range2448238 1 913 1041 annotation2448234 1 psbQ range2448234 1 20 553 annotation2448233 1 rbs range2448233 1 1 19 annotation2448237 1 psbR range2448237 1 571 912 BBa_K1640021_sequence 1 aagaaggagatatacatatggctaccaccgttcgcagcgaggttgccaaaaaagttgccatgttgtctactctgccggccacgctggctgctcatccggcctttgcgttggtcgatgaacgtatgaatggagacggaacgggtcgcccatttggtgtgaacgatcccgttttgggctgggttctgctgggcgtgtttgggaccatgtgggcgatttggttcattgggcaaaaggacttgggcgattttgaagatgctgacgacggcttgaaactgtgaaggaggtaaaaaaatgacaaccctggcgctggtgctggcgaaattgccggaagcatatgcgccgtttgccccgatcgtagacgtactgcccgttatcccagtgtttttcatcctgttagcgtttgtttggcaggcggccgtgtcattccgttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1640022_sequence 1 agaagaaggagatatacatatggcgagtggcagtgatgtcagccgtcgtgcagcactggccggatttgctggcgcagccgccctggtgtcaagctccccggccaatgcggcatatggggactcagccaatgtgttcggtaaagtgaccaataaaagcggcttcgttccatacgccggtgatggatttgcgctgttgctgcccgccaaatggaaccctagcaaagagaacgattttccaggtgtcatcctgcgttatgaagataactttgatgcggtgaataacttggtagtcatcgcgcaagatacggataaaaaggcaattgcggactttggcagccaggacaaatttcttgaatcggtaagctatcttctcggcaagcaggcgtatagcggcgagactcaatcggagggtggctttgcgccgaaccgtgtgtcggccgccagccttttggacgtgtcgactactacggataagaaagggaaaacgtactataaatatgagctgctggtacgttccgcggatggtgatgaagggggccggcatcaactgatcggcgccacggtaggaagtgataataaactgtatatcattaaaatccaaatcggcgataaacgctggtttaaaggagccaaaaaggaagcaatgggcgcatttgattcattcactgtggtttaa BBa_K1998007_sequence 1 ggctttacactttatgcttccggctcgtatgttgtgtggaaagaaggagatatacatatggaagttaacatttatggcctgactgccacagccttgttcatcatcattcctacgagtttcctgttgatcctgtatgtgaaaacagcgagcacccaagactgaaggaggtaaaaaaatggtcggggtccctgtagtcttcgcaacgccaaatggctggacggataataaaggcgcagtctttagtggcctttcgctgtggctgttactggtctttgttgtcggtattctgaactcattcgtagtttaaaggaggtaaaaaaatgtcagaagccggtaaagtgctgccgggctggggtaccaccgttctgatggcagttttcatcctgctgtttgcggcgtttcttttaattattctggaaatctataattcttcactgatcctggatgatgtgtccatgtcgtgggaaactttagcaaaagtctcgtgatactagagaagaaggagatatacatatggctaccaccgttcgcagcgaggttgccaaaaaagttgccatgttgtctactctgccggccacgctggctgctcatccggcctttgcgttggtcgatgaacgtatgaatggagacggaacgggtcgcccatttggtgtgaacgatcccgttttgggctgggttctgctgggcgtgtttgggaccatgtgggcgatttggttcattgggcaaaaggacttgggcgattttgaagatgctgacgacggcttgaaactgtgaaggaggtaaaaaaatgacaaccctggcgctggtgctggcgaaattgccggaagcatatgcgccgtttgccccgatcgtagacgtactgcccgttatcccagtgtttttcatcctgttagcgtttgtttggcaggcggccgtgtcattccgttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggctttacactttatgcttccggctcgtatgttgtgtggaaagaaggagatatacatatggcccagaaggtgggtcaggcggctgctgctgcggccctggcaacggccatggtagcgggctcggcgaacgccctgacgtttgacgaaatccaaggcttaacctacctccaagtcaaaggttcgggtattgcgaacacctgcccggtcctggaatccggcacgaccaacctgaaagagttgaaggcgggcagttataaactggaaaatttctgcatcgagcccacgtcatttaccgttaaggaagaaagtcagtttaaaggtggcgaaacggaatttgtgaaaacaaagctgatgacccgtttaacctacacgctcgatgcgatgtcgggtagttttaaagtaggtagcgatggcagcgccgagctgaaagaggatgatggcatcgactatgctgcaaccacggtacagctgccgggcggcgagcgggtagctttcctgttcaccattaaacagttcgatggcaaagggacgctggataatattaagggtgattttttagtgccgtcttatcgcgggagctctttcctcgatccaaaaggccgtggcgggagcaccggctacgataacgcagtggccctccctgcacgtgcggacgcggaagaacttttaaaggaaaacgttaaaatcaccaaagcgttaaagggctcggcggtgtttagcgtggcgaaagtcgatccggtgacgggcgaaattgccggcgtatttgaaagcatccagccttcagataccgacctgggtgcgaaaccgccgaaggatatcaaggtgactggcctgtggtatgcacaattaaaataatactagagagaagaaggagatatacatatggcgagtggcagtgatgtcagccgtcgtgcagcactggccggatttgctggcgcagccgccctggtgtcaagctccccggccaatgcggcatatggggactcagccaatgtgttcggtaaagtgaccaataaaagcggcttcgttccatacgccggtgatggatttgcgctgttgctgcccgccaaatggaaccctagcaaagagaacgattttccaggtgtcatcctgcgttatgaagataactttgatgcggtgaataacttggtagtcatcgcgcaagatacggataaaaaggcaattgcggactttggcagccaggacaaatttcttgaatcggtaagctatcttctcggcaagcaggcgtatagcggcgagactcaatcggagggtggctttgcgccgaaccgtgtgtcggccgccagccttttggacgtgtcgactactacggataagaaagggaaaacgtactataaatatgagctgctggtacgttccgcggatggtgatgaagggggccggcatcaactgatcggcgccacggtaggaagtgataataaactgtatatcattaaaatccaaatcggcgataaacgctggtttaaaggagccaaaaaggaagcaatgggcgcatttgattcattcactgtggtttaatactagagagaagaaggagatatacatatggcatccggtgagtcacgccgcgcggtgctgggtggtctgttggcaagcgccgttgcagcggtggcgcctaaagcggctcttgcattaactccggtagacctgttcgacgatcggtctgtccgggaccgtggattcgatcttatctatgaggcacgggatcttgatcttcctcagaacgtgcgcgagggcttcacacaggcacgtgcgtccctcgacgagactaagaaacgcgtaaaagaaagtgaagctcgtattgatgcggacctggatgtatttatccagaaaagctactggaccgaagcccgtgaacaactgcgtcgccaagtgggcaccctgcgttttgatctgaacaccttagctagtaccaaggaaaaagaagcgaagaaggcagcgctgggtttacgtaaagaatttatccaagccgtggaagatcttgattttgctctccgcgaaaaggatcaagcgagcgctgcgaagaaacttgaaatcactaaagcaaaactggactcagtactcgctgcggtgctctaaaagaaggagatatacatatgggcggggggaaaaccgatattacaaaggtgggtttgaactctattgaggacccggttgtcaaacagaatctgatgggtaagagtcgtttcatgaataagaaagattggaaagacgcgtcaggccgcaaaggcaaaggttatggtgtttatcgctatgaggataaatacggagctaatgtcgatggttacagccctatctataccccagatctctggaccgaatcaggtgactcgtataccctgggcaccaaggggctgattgcgtgggcaggtctcgttttggtgctgcttgcagtgggagttaaccttattattagtacttcccagctgggtgcgtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1998006_sequence 1 ggctttacactttatgcttccggctcgtatgttgtgtggaaagaaggagatatacatatggcccagaaggtgggtcaggcggctgctgctgcggccctggcaacggccatggtagcgggctcggcgaacgccctgacgtttgacgaaatccaaggcttaacctacctccaagtcaaaggttcgggtattgcgaacacctgcccggtcctggaatccggcacgaccaacctgaaagagttgaaggcgggcagttataaactggaaaatttctgcatcgagcccacgtcatttaccgttaaggaagaaagtcagtttaaaggtggcgaaacggaatttgtgaaaacaaagctgatgacccgtttaacctacacgctcgatgcgatgtcgggtagttttaaagtaggtagcgatggcagcgccgagctgaaagaggatgatggcatcgactatgctgcaaccacggtacagctgccgggcggcgagcgggtagctttcctgttcaccattaaacagttcgatggcaaagggacgctggataatattaagggtgattttttagtgccgtcttatcgcgggagctctttcctcgatccaaaaggccgtggcgggagcaccggctacgataacgcagtggccctccctgcacgtgcggacgcggaagaacttttaaaggaaaacgttaaaatcaccaaagcgttaaagggctcggcggtgtttagcgtggcgaaagtcgatccggtgacgggcgaaattgccggcgtatttgaaagcatccagccttcagataccgacctgggtgcgaaaccgccgaaggatatcaaggtgactggcctgtggtatgcacaattaaaataatactagagagaagaaggagatatacatatggcgagtggcagtgatgtcagccgtcgtgcagcactggccggatttgctggcgcagccgccctggtgtcaagctccccggccaatgcggcatatggggactcagccaatgtgttcggtaaagtgaccaataaaagcggcttcgttccatacgccggtgatggatttgcgctgttgctgcccgccaaatggaaccctagcaaagagaacgattttccaggtgtcatcctgcgttatgaagataactttgatgcggtgaataacttggtagtcatcgcgcaagatacggataaaaaggcaattgcggactttggcagccaggacaaatttcttgaatcggtaagctatcttctcggcaagcaggcgtatagcggcgagactcaatcggagggtggctttgcgccgaaccgtgtgtcggccgccagccttttggacgtgtcgactactacggataagaaagggaaaacgtactataaatatgagctgctggtacgttccgcggatggtgatgaagggggccggcatcaactgatcggcgccacggtaggaagtgataataaactgtatatcattaaaatccaaatcggcgataaacgctggtttaaaggagccaaaaaggaagcaatgggcgcatttgattcattcactgtggtttaatactagagagaagaaggagatatacatatggcatccggtgagtcacgccgcgcggtgctgggtggtctgttggcaagcgccgttgcagcggtggcgcctaaagcggctcttgcattaactccggtagacctgttcgacgatcggtctgtccgggaccgtggattcgatcttatctatgaggcacgggatcttgatcttcctcagaacgtgcgcgagggcttcacacaggcacgtgcgtccctcgacgagactaagaaacgcgtaaaagaaagtgaagctcgtattgatgcggacctggatgtatttatccagaaaagctactggaccgaagcccgtgaacaactgcgtcgccaagtgggcaccctgcgttttgatctgaacaccttagctagtaccaaggaaaaagaagcgaagaaggcagcgctgggtttacgtaaagaatttatccaagccgtggaagatcttgattttgctctccgcgaaaaggatcaagcgagcgctgcgaagaaacttgaaatcactaaagcaaaactggactcagtactcgctgcggtgctctaaaagaaggagatatacatatgggcggggggaaaaccgatattacaaaggtgggtttgaactctattgaggacccggttgtcaaacagaatctgatgggtaagagtcgtttcatgaataagaaagattggaaagacgcgtcaggccgcaaaggcaaaggttatggtgtttatcgctatgaggataaatacggagctaatgtcgatggttacagccctatctataccccagatctctggaccgaatcaggtgactcgtataccctgggcaccaaggggctgattgcgtgggcaggtctcgttttggtgctgcttgcagtgggagttaaccttattattagtacttcccagctgggtgcgtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1640023_sequence 1 ggctttacactttatgcttccggctcgtatgttgtgtggaaagaaggagatatacatatggcccagaaggtgggtcaggcggctgctgctgcggccctggcaacggccatggtagcgggctcggcgaacgccctgacgtttgacgaaatccaaggcttaacctacctccaagtcaaaggttcgggtattgcgaacacctgcccggtcctggaatccggcacgaccaacctgaaagagttgaaggcgggcagttataaactggaaaatttctgcatcgagcccacgtcatttaccgttaaggaagaaagtcagtttaaaggtggcgaaacggaatttgtgaaaacaaagctgatgacccgtttaacctacacgctcgatgcgatgtcgggtagttttaaagtaggtagcgatggcagcgccgagctgaaagaggatgatggcatcgactatgctgcaaccacggtacagctgccgggcggcgagcgggtagctttcctgttcaccattaaacagttcgatggcaaagggacgctggataatattaagggtgattttttagtgccgtcttatcgcgggagctctttcctcgatccaaaaggccgtggcgggagcaccggctacgataacgcagtggccctccctgcacgtgcggacgcggaagaacttttaaaggaaaacgttaaaatcaccaaagcgttaaagggctcggcggtgtttagcgtggcgaaagtcgatccggtgacgggcgaaattgccggcgtatttgaaagcatccagccttcagataccgacctgggtgcgaaaccgccgaaggatatcaaggtgactggcctgtggtatgcacaattaaaataa BBa_K1640012_sequence 1 agaagaaggagatatacatatggcatccggtgagtcacgccgcgcggtgctgggtggtctgttggcaagcgccgttgcagcggtggcgcctaaagcggctcttgcattaactccggtagacctgttcgacgatcggtctgtccgggaccgtggattcgatcttatctatgaggcacgggatcttgatcttcctcagaacgtgcgcgagggcttcacacaggcacgtgcgtccctcgacgagactaagaaacgcgtaaaagaaagtgaagctcgtattgatgcggacctggatgtatttatccagaaaagctactggaccgaagcccgtgaacaactgcgtcgccaagtgggcaccctgcgttttgatctgaacaccttagctagtaccaaggaaaaagaagcgaagaaggcagcgctgggtttacgtaaagaatttatccaagccgtggaagatcttgattttgctctccgcgaaaaggatcaagcgagcgctgcgaagaaacttgaaatcactaaagcaaaactggactcagtactcgctgcggtgctctaaaagaaggagatatacatatgggcggggggaaaaccgatattacaaaggtgggtttgaactctattgaggacccggttgtcaaacagaatctgatgggtaagagtcgtttcatgaataagaaagattggaaagacgcgtcaggccgcaaaggcaaaggttatggtgtttatcgctatgaggataaatacggagctaatgtcgatggttacagccctatctataccccagatctctggaccgaatcaggtgactcgtataccctgggcaccaaggggctgattgcgtgggcaggtctcgttttggtgctgcttgcagtgggagttaaccttattattagtacttcccagctgggtgcgtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1998004_sequence 1 ggctttacactttatgcttccggctcgtatgttgtgtggaaagaaggagatatacatatggaagttaacatttatggcctgactgccacagccttgttcatcatcattcctacgagtttcctgttgatcctgtatgtgaaaacagcgagcacccaagactgaaggaggtaaaaaaatggtcggggtccctgtagtcttcgcaacgccaaatggctggacggataataaaggcgcagtctttagtggcctttcgctgtggctgttactggtctttgttgtcggtattctgaactcattcgtagtttaaaggaggtaaaaaaatgtcagaagccggtaaagtgctgccgggctggggtaccaccgttctgatggcagttttcatcctgctgtttgcggcgtttcttttaattattctggaaatctataattcttcactgatcctggatgatgtgtccatgtcgtgggaaactttagcaaaagtctcgtgatactagagaagaaggagatatacatatggctaccaccgttcgcagcgaggttgccaaaaaagttgccatgttgtctactctgccggccacgctggctgctcatccggcctttgcgttggtcgatgaacgtatgaatggagacggaacgggtcgcccatttggtgtgaacgatcccgttttgggctgggttctgctgggcgtgtttgggaccatgtgggcgatttggttcattgggcaaaaggacttgggcgattttgaagatgctgacgacggcttgaaactgtgaaggaggtaaaaaaatgacaaccctggcgctggtgctggcgaaattgccggaagcatatgcgccgtttgccccgatcgtagacgtactgcccgttatcccagtgtttttcatcctgttagcgtttgtttggcaggcggccgtgtcattccgttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1640008_sequence 1 ggctttacactttatgcttccggctcgtatgttgtgtggaaagaaggagatatacatatggaagttaacatttatggcctgactgccacagccttgttcatcatcattcctacgagtttcctgttgatcctgtatgtgaaaacagcgagcacccaagactgaaggaggtaaaaaaatggtcggggtccctgtagtcttcgcaacgccaaatggctggacggataataaaggcgcagtctttagtggcctttcgctgtggctgttactggtctttgttgtcggtattctgaactcattcgtagtttaaaggaggtaaaaaaatgtcagaagccggtaaagtgctgccgggctggggtaccaccgttctgatggcagttttcatcctgctgtttgcggcgtttcttttaattattctggaaatctataattcttcactgatcctggatgatgtgtccatgtcgtgggaaactttagcaaaagtctcgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z