BBa_K1640006 1 BBa_K1640006 psbELJ 2015-09-09T11:00:00Z 2015-09-09T11:28:11Z N.A n/A false false _2057_ 16353 28565 9 false N/A false Meghan Cook annotation2475062 1 Plac range2475062 1 3 42 annotation2448206 1 psbE range2448206 1 56 304 annotation2448218 1 double terminator range2448218 1 577 705 annotation2448217 1 psbJ range2448217 1 448 576 annotation2448215 1 psbL range2448215 1 318 434 annotation2448205 1 rbs range2448205 1 43 55 annotation2448216 1 rbs range2448216 1 435 447 annotation2448214 1 rbs range2448214 1 305 317 BBa_K1998002 1 BBa_K1998002 psbTB 2016-10-11T11:00:00Z 2016-10-17T09:52:35Z The genes were sourced from Chlamydomonas rienhardtii and synthesised This part is composed of the psbT and psbB genes. The psbT protein's function is not well known however it appears to be involved in the dimerization of Photosystem II and is essential for photosynthetic activity. false false _2465_ 33907 33907 9 false Genes were codon optimised for bacteria using optimiser tool at http://genomes.urv.es/ OPTIMIZER/ Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence. false Shauna Winchester annotation2516384 1 Start range2516384 1 57 59 annotation2516402 1 psbB range2516402 1 165 1810 annotation2514095 1 psbT range2514095 1 60 164 annotation2516383 1 RBS range2516383 1 43 56 annotation2514094 1 Plac range2514094 1 1 41 BBa_K1998008 1 BBa_K1998008 psbELJ-TB 2016-10-11T11:00:00Z 2016-10-17T01:26:21Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. This part contains the psbELJ and psbTB operons found in the photosystem II pathway. The biobrick contains psbE, psbL, and psbJ, driven by Plac promoter, with RBS preceeding each gene, and terminator at the 3' end. The second operon is composed of the psbT and psbB genes. The psbT protein's function is not well known however it appears to be involved in the dimerization of Photosystem II and is essential for photosynthetic activity. false false _2465_ 33907 33907 9 false Genes were codon optimised for bacteria using optimiser tool false Shauna Winchester component2514110 1 BBa_K1998002 component2514107 1 BBa_K1640006 annotation2514107 1 BBa_K1640006 range2514107 1 1 705 annotation2514110 1 BBa_K1998002 range2514110 1 714 2055 BBa_K1998002_sequence 1 gggctttacactttatgcttccggctcgtatgttgtgtggaaggaggtaaacatatggaagcactggtctatacatttttgttggtagggacactggggatcattttcttctctattttttttcgtgaccccccgcgcatgattaagtaaaggaggtataaatatgggtttaccttggtatcgtgtacatacagtagtaatcaatgaccctgggcgattaatctctgtgcacttaatgcacacagcgttagtttctggttgggcaggttctatggctttatttgaaatttcagtctttgatccatctgacccagtattaaacccaatgtggcgtcaaggtatgttcgttctacctttcatgacacgtttaggtatcacacaatcttggggtggttggactattagcggtgaaacagctacaaacccaggtatctggagttatgaaggtgttgcagcagctcacattattctttcaggtgcgttattcttagcttcagtatggcactggacatactgggatttagaacttttccgtgacccacgtacaggtaaaacagctttagacttaccaaagatcttcggtattcacttattcttatctggtttactatgttttggtttcggtgcattccacgtaactggtgtgtttggtcctggtatttgggtttcagacccttacggtttaacaggtagggttcaaccagtagctccttcttggggtgctgatggttttgacccatacaacccaggtggtattgcttcacaccacatcgcagctggtattttaggtgttttagcaggtttattccacttatgtgtacgtccttctattcgtttatacttcggtttatcaatgggtagtattgaaactgtattatcaagcagtattgcagcagtattctgggcagctttcgttgtagcaggtactatgtggtacggttcagcagctactccaattgaattatttggtccaactcgttaccagtgggaccaaggtttcttccaacaagaaattcagaaacgtgtacaagctagtttagctgaaggtgcttcattatctgatgcatggtcacgtattccagagaaattagctttctatgattacattggtaataacccagctaaaggtggtttattccgtactggtgctatgaacagtggtgatggtatcgctgtaggttggttaggtcacgcatcattcaaagatcaagaaggtcgcgaattattcgttcgtcgtatgccaacattctttgaaacattcccagttcttttattagataaagatggtattgttcgtgctgacgttcctttccgtaaagctgaatctaaatatagtattgaacaagtaggtgtatctgttacattctacggtggtgaattagatggtttaacatttactgatccagctacagttaagaaatatgctcgtaaagctcaattaggtgaaatctttgaatttgaccgttcaactttacaatctgatggtgttttccgtagtagcccacgtggttggtttacttttggtcacgtatgttttgcattattattcttctttggtcacatttggcacggtgctcgtacgatcttccgtgacgtatttgctggtattgatgatgatattaatgaccaagttgagtttggtaaatacaaaaaacttggtgatacttcttctctacgtgaagcattctaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt BBa_K1640006_sequence 1 agggctttacactttatgcttccggctcgtatgttgtgtggaaggaggtaaaaaaatggcgggtaaaccggttgaacgtccgttctctgacatcctgacctctatccgttactgggttatccactctatcaccgttccggcgctgttcatcgcgggttggctgttcgtttctaccggtctggcgtacgacgttttcggtaccccgcgtccgaacgaatacttcaccgaagaccgtcaggaagcgccgctgatcaccgaccgtttcaacgcgctggaacaggttaaaaaactgtctggtaactaaaggaggtaaaaaaatggcacgtccgaatccgaataaacaggttgttgaactgaatcgtaccagcctgtattggggtctgctgctgatttttgttctggcagttctgtttagcagctatatttttaattaaaggaggtaaaaaaatgagcaataccggtaccaccggtcgtattccgctgtggctggttggtaccgttgttggtaccctggcaattggtctgctggcagtttttttttatggtagctatgttggtctgggtagcagcctgtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1998008_sequence 1 agggctttacactttatgcttccggctcgtatgttgtgtggaaggaggtaaaaaaatggcgggtaaaccggttgaacgtccgttctctgacatcctgacctctatccgttactgggttatccactctatcaccgttccggcgctgttcatcgcgggttggctgttcgtttctaccggtctggcgtacgacgttttcggtaccccgcgtccgaacgaatacttcaccgaagaccgtcaggaagcgccgctgatcaccgaccgtttcaacgcgctggaacaggttaaaaaactgtctggtaactaaaggaggtaaaaaaatggcacgtccgaatccgaataaacaggttgttgaactgaatcgtaccagcctgtattggggtctgctgctgatttttgttctggcagttctgtttagcagctatatttttaattaaaggaggtaaaaaaatgagcaataccggtaccaccggtcgtattccgctgtggctggttggtaccgttgttggtaccctggcaattggtctgctggcagtttttttttatggtagctatgttggtctgggtagcagcctgtaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggggctttacactttatgcttccggctcgtatgttgtgtggaaggaggtaaacatatggaagcactggtctatacatttttgttggtagggacactggggatcattttcttctctattttttttcgtgaccccccgcgcatgattaagtaaaggaggtataaatatgggtttaccttggtatcgtgtacatacagtagtaatcaatgaccctgggcgattaatctctgtgcacttaatgcacacagcgttagtttctggttgggcaggttctatggctttatttgaaatttcagtctttgatccatctgacccagtattaaacccaatgtggcgtcaaggtatgttcgttctacctttcatgacacgtttaggtatcacacaatcttggggtggttggactattagcggtgaaacagctacaaacccaggtatctggagttatgaaggtgttgcagcagctcacattattctttcaggtgcgttattcttagcttcagtatggcactggacatactgggatttagaacttttccgtgacccacgtacaggtaaaacagctttagacttaccaaagatcttcggtattcacttattcttatctggtttactatgttttggtttcggtgcattccacgtaactggtgtgtttggtcctggtatttgggtttcagacccttacggtttaacaggtagggttcaaccagtagctccttcttggggtgctgatggttttgacccatacaacccaggtggtattgcttcacaccacatcgcagctggtattttaggtgttttagcaggtttattccacttatgtgtacgtccttctattcgtttatacttcggtttatcaatgggtagtattgaaactgtattatcaagcagtattgcagcagtattctgggcagctttcgttgtagcaggtactatgtggtacggttcagcagctactccaattgaattatttggtccaactcgttaccagtggggaccaaggtttcttccaacaagaaattcaaaaacgtgtacaagctagtttagctgaaaggggcttcattatctgatgcatgggccacgtattccagagaaaattagctttctatgaattactttgggaaaaacccccgctaagagggggtttattccgcatcggtgctataaacaaggggggagggattccctttaaggttggttaggggcgcccctcttttcaaaaaacaaagaggcccgaaattatccttccccggcaggcgcaacatttttgaaaaaatcccccctctttttataaaaaaaaagaagaatgttttgcggcgggagtcctcttcctttcaggtgtattaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z