BBa_K1080012
1
DVR1
DVR1
2013-09-21T11:00:00Z
2015-05-08T01:09:03Z
Chlamydomonas reinhardtii
THSHERRNBHYDF
false
false
_1389_
0
18612
9
In stock
false
STHSHTSHD
false
Macquarie University
annotation2525687
1
RBS
range2525687
1
6
16
annotation2525688
1
DVR1
range2525688
1
25
1000
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
BBa_K1080009
1
"G"
ChlG
2013-09-21T11:00:00Z
2016-10-20T07:21:10Z
Chlamydomonas reinhardtii
FSDBSDGBGDS
false
false
_1389_
7708
18612
9
In stock
false
DFSHGDSS
false
Macquarie University
annotation2525691
1
RBS
range2525691
1
6
16
annotation2525692
1
ChlG
range2525692
1
25
1044
BBa_K1080008
1
"The Big P
ChlP
2013-09-21T11:00:00Z
2016-10-20T07:22:47Z
Chlamydomonas reinhardtii
Long Description
false
false
_1389_
7708
18463
9
In stock
false
Design Consideration
false
Macquarie University
annotation2525690
1
ChlP
range2525690
1
25
1293
annotation2525689
1
RBS
range2525689
1
6
16
BBa_K1998013
1
BBa_K1998013
MgPPIX to Chlorophyll <i>a</i> plasmid
2016-10-12T11:00:00Z
2016-10-19T06:06:16Z
The genes were sourced from Chlamydomonas rienhardtii and synthesised.
This composite part makes up one of our operons in the chlorophyll biosynthesis pathway. It is composed of YCF54, ChlM, POR, DVR1, ChlP and ChlG.
false
false
_2465_
33907
33907
9
false
Codon optimisation, order of parts in operon, illegal enzyme kinetics
false
Shauna Winchester
component2523158
1
BBa_K1080008
component2523159
1
BBa_K1080009
component2523145
1
BBa_K1080010
component2523148
1
BBa_K1640018
component2523156
1
BBa_K1080007
component2523157
1
BBa_K1080012
component2523149
1
BBa_R0010
component2523138
1
BBa_R0010
annotation2523159
1
BBa_K1080009
range2523159
1
5262
6311
annotation2523157
1
BBa_K1080012
range2523157
1
2841
3946
annotation2523156
1
BBa_K1080007
range2523156
1
1766
2832
annotation2523149
1
BBa_R0010
range2523149
1
1558
1757
annotation2523145
1
BBa_K1080010
range2523145
1
209
679
annotation2523158
1
BBa_K1080008
range2523158
1
3955
5253
annotation2523148
1
BBa_K1640018
range2523148
1
688
1549
annotation2523138
1
BBa_R0010
range2523138
1
1
200
BBa_K1640018
1
BBa_K1640018
CHlM
2015-09-09T11:00:00Z
2016-10-20T07:15:09Z
n/a
n/a
false
false
_2057_
7708
28565
9
true
n/a
false
Meghan Cook
annotation2474919
1
ChlM
range2474919
1
20
862
annotation2474918
1
RBS
range2474918
1
1
11
BBa_K1080010
1
"YCF"
YCF54
2013-09-21T11:00:00Z
2016-10-20T07:15:17Z
Chlamydomonas reinhardtii
Long Description here
false
false
_1389_
7708
18463
9
In stock
false
Design Considerations here
false
Macquarie University
annotation2525683
1
RBS
range2525683
1
6
16
annotation2525684
1
25
range2525684
1
25
471
BBa_K1080007
1
POR
POR
2013-09-21T11:00:00Z
2016-10-20T07:16:42Z
Chlamydomonas reinhardtii
DFGBDTD
false
false
_1389_
7708
18612
9
It's complicated
false
DFGSGBRF
false
Macquarie University
annotation2525685
1
RBS
range2525685
1
6
16
annotation2525686
1
POR
range2525686
1
25
1064
BBa_K1080010_sequence
1
agctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K1080007_sequence
1
agctttaagaaggagatatacataatggttgtttgcgcagcaactgcaactgctccttctccatctctggctgataagttcaagcctaacgcaatcgcacgtgttccagcaacccagcagaagcagacagctatcatcaccggtgcaagctctggtctgggtctgaacgcagcaaaggctctggctgctaccggtgaatggcatgttgtaatggcatgccgtgacttcctcaaggctgagcaggctgctaagaaggtcggtatgccagcaggttcctactcaatcctgcacctggacctgtcctctctggaatctgttcgtcagttcgtgcagaacttcaaggcatctggtcgtcgtctggatgcactggtgtgcaacgctgctgtgtacctgccaacagcaaaggaacctcgcttcaccgcagacggcttcgaactgtctgtgggtaccaaccatcttggtcacttcctgctgaccaacctgctgctggatgacctgaagaacgcacctaacaagcaacctcgttgcattattgtcggttccatcacaggtaacaccaacactctggcaggtaacgtgccaccaaaggccaacctgggagatctgtcgggcctcgcagcaggtgtgccagcagctaatccaatgatggatggtcaagaatttaacggtgctaaggcctacaaggattccaaggttgcatgcatgatgaccgtgcgccagatgcatcagcgtttccatgatgcaaccggcatcaccttcgcatcgctgtacccaggctgcattgccgagactggtctgtttcgtgaacatgtgccactgttcaagactctgtttccaccattccagaagtacatcaccaagggttacgtgtcggaagaagaagctggtcgtcgtctggcagcagtcatctctgatcctaagctgaacaagtctggtgcatactggtcttggtcttccaccactgggtcgttcgacaacaagtgtcttaagaagtggccgaagaatccaaggcctcccacctgtgggaaatctctgccaagctggtgggcctgagcgcgtaataataa
BBa_K1080008_sequence
1
agctttaagaaggagatatacataatggtcatcggtggtggtccatctggtgcatgcgcagcagagaccctggctaagggtggtgtggaaaccttcctgctggaacgcaagctggacaactgcaagccatgcggtggtgccatccctctgtgcatggttgaagagttcgacctgcctatggagattattgaccgtcgcgtcaccaagatgaagatgatctctccttccaaccgtgaagtcgatgtgggtaagacactcagcgaaacagagtggattggtatgtgccgtcgtgaagtgtttgatgactacctgcgtaaccgtgcacagaagctgggtgctaacatcgtgaacggtctgttcatgcgttctgagcagcagagcgctgaaggtcctttcaccatccactacaacagctacgaggatggtagcaagatgggtaagcctgctacactggaagtcgacatgatcatcggtgctgacggtgcaaacagccgtatcgccaaggagatcgacgctggcgagtacgactacgcaattgcattccaggaacgtatccgtatcccagatgacaagatgaagtactatgagaacctggctgagatgtacgttggtgacgacgtgtctccagacttctacggatgggtgttccctaagtacgaccacgttgcagttggtacaggtacagttgtcaacaagacagctatcaagcagtaccagcaggctacacgtgatcgctctaaggtcaagaccgaaggtggtaagatcatccgtgttgaggcacatccaatcccagagcatccacgtccacgtcgttgcaagggtcgtgttgcactggtcggtgacgctgctggttacgtgaccaagtgctctggtgaaggtatctacttcgctgctaagtctggtcgtatggcagctgaagctattgtggaaggttctgctaacggaactaagatgtgcggtgaagatgcaatccgtgtctacctggacaaatgggatcgtaagtactggacaacatacaaggttctggacatcctgcaaaaggttttctaccgatccaatcctgcacgtgaggcattcgtggagctgtgcgaagacagctacgtgcagaagatgacatttgactcgtacctgtacaagaccgtggtgccaggtaacccactggacgacgtcaagctgctggttcgtaccgtgtcttccatcctgcgttccaacgcactgcgtagcgttaactccaagtcggtgaacgtgtcgttcggctccaaggcgaacgaagaacgtgtcatggctgcgtaataataa
BBa_K1080009_sequence
1
agctttaagaaggagatatacataatgaaccagcaagctaccgaagaaaagagcgacaccaacagcgctgctcgccagatgctgggcatgaagggtgctgccttggagacggacatctggaaaatccgcgtgcagcttaccaagcctgtgacttggatccctctcatttggggtgttgcttgcggtgcagcagcttctggtcactaccagtggaacaaccctacacaaattgcgcagctgctgacctgcatgatgatgtctggtccattcctgactggctacactcagactattaacgactggtatgaccgtgagattgatgcaatcaacgaaccttaccgtccaattccatctggtcgtatctctgaacgtgacgtgatcgtgcagatctgggtgctgcttctaggtggtattggtctggcatacacacttgaccagtgggctggtcacacaacgcctgtgatgcttcagctgaccatcttcggctccttcatctcttacatctactctgcaccacctctcaagctcaagcagagcggttgggcaggtaactacgcacttggttcgtcatacatcgcactgccatggtgggcaggtcaggctctgtttggcactctgaccctggacgtgatggctctgaccatcgcatactcgctggcaggtctgggcattgccattgtgaacgacttcaagtccattgaaggtgacaggcagatgggtcttcagtcgctgcctgtggcgttcggtgtggacacggctaagtggatttgcgtgtccaccatcgacgtgacgcagcttggtgtagctgcctaccttgcctggggtctgcacgaggagctgtatggcgcggtgctgctggcgctcatcctgccgcagatctacttccagtacaagtacttcctgccagatccaatcgccaacgacgtcaagtaccaggcatccgcacagccattccttgtgttcggcctgctcacagcaggtctggcatgcggtcaccacgtcaacgcagtggcagcagcagcttcagcagctggtgcactgtaataataa
BBa_K1640018_sequence
1
agaagaaggagatatacatatggcttctgagattgctcaaactgccgatgttggttcgctgaccttcgctgttggtggagttggtgctgttgtaggtctgggtgcactgcttgtcgctactgatcctcagaagcgccgctcggaacagatgaagtcattcgatggtgatgagaaggaagcagtgaaggactacttcaacactgctggcttcgaacgttggcgtaagatctacggtgagacagacgaagtgaacaaggttcagcttgacatccgtactggccatgcacagacagttgacaaggttctgcgttgggtagacgaagaaggtagcgtacaaggtatcacagttgcagactgcggttgcggtacaggtagcctggctatcccgctggcactgcgtggcgcagctgtcagcgcatctgacatctctgctgcaatggcgtctgaagctgagcagcgttaccaacaagctgttgcagcaggtcaaggtaaggcacctaaggttgcacctaagttcgaagctcttgatctggaatctgtcaaaggtaagtacgacacggtcacttgcctggacgtgatgattcactacccccaggacaaggtggacgccatgatcacgcacctggcaggtctgtctgatcgtcgtctcatcatctcgttcgcacctaagacgctgtcgtacagcatcctcaagcgcattggtgaactgttccctggtccaagcaaggccacacgtgcatacctgcaccgtgaagaagacgtggaagctgctctcaagcgtgcaggtttcaaggtcaccaagcgtgaaatgaccgcaaccagcttctacttctcgcgcctgctggaagctatccgtgaataa
BBa_K1080012_sequence
1
agctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataa
BBa_K1998013_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggctccagctgctgcatcggcagacaaggctaccgctgcggagtactatgctctggtgtgcaacgctgagtggttcttcatggatccacagaacgagtctgtggctgagcagctgcgcgagaaggttcgcttcttcaaggagcagaacaaggagcgcgacttcttcatcgtgcctaaccctaagtggcttgacgctaagttccctgagcaggctaagcaggtgaagcgtccatgcgtggcactggtctctaccgacaagatgtggatcacgttcatgaagctgcgtctggatcgtgtgctgaagatcgaccttaagtctatgcctgcatcggaggtgctggcagctggtgaagctctgcctgacttcaagcctgatggtaagtggactgctccttacgcccggtacactcctggttggtggaacgtgttcctgcctaaccattaatactagagagaagaaggagatatacatatggcttctgagattgctcaaactgccgatgttggttcgctgaccttcgctgttggtggagttggtgctgttgtaggtctgggtgcactgcttgtcgctactgatcctcagaagcgccgctcggaacagatgaagtcattcgatggtgatgagaaggaagcagtgaaggactacttcaacactgctggcttcgaacgttggcgtaagatctacggtgagacagacgaagtgaacaaggttcagcttgacatccgtactggccatgcacagacagttgacaaggttctgcgttgggtagacgaagaaggtagcgtacaaggtatcacagttgcagactgcggttgcggtacaggtagcctggctatcccgctggcactgcgtggcgcagctgtcagcgcatctgacatctctgctgcaatggcgtctgaagctgagcagcgttaccaacaagctgttgcagcaggtcaaggtaaggcacctaaggttgcacctaagttcgaagctcttgatctggaatctgtcaaaggtaagtacgacacggtcacttgcctggacgtgatgattcactacccccaggacaaggtggacgccatgatcacgcacctggcaggtctgtctgatcgtcgtctcatcatctcgttcgcacctaagacgctgtcgtacagcatcctcaagcgcattggtgaactgttccctggtccaagcaaggccacacgtgcatacctgcaccgtgaagaagacgtggaagctgctctcaagcgtgcaggtttcaaggtcaccaagcgtgaaatgaccgcaaccagcttctacttctcgcgcctgctggaagctatccgtgaataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagagctttaagaaggagatatacataatggttgtttgcgcagcaactgcaactgctccttctccatctctggctgataagttcaagcctaacgcaatcgcacgtgttccagcaacccagcagaagcagacagctatcatcaccggtgcaagctctggtctgggtctgaacgcagcaaaggctctggctgctaccggtgaatggcatgttgtaatggcatgccgtgacttcctcaaggctgagcaggctgctaagaaggtcggtatgccagcaggttcctactcaatcctgcacctggacctgtcctctctggaatctgttcgtcagttcgtgcagaacttcaaggcatctggtcgtcgtctggatgcactggtgtgcaacgctgctgtgtacctgccaacagcaaaggaacctcgcttcaccgcagacggcttcgaactgtctgtgggtaccaaccatcttggtcacttcctgctgaccaacctgctgctggatgacctgaagaacgcacctaacaagcaacctcgttgcattattgtcggttccatcacaggtaacaccaacactctggcaggtaacgtgccaccaaaggccaacctgggagatctgtcgggcctcgcagcaggtgtgccagcagctaatccaatgatggatggtcaagaatttaacggtgctaaggcctacaaggattccaaggttgcatgcatgatgaccgtgcgccagatgcatcagcgtttccatgatgcaaccggcatcaccttcgcatcgctgtacccaggctgcattgccgagactggtctgtttcgtgaacatgtgccactgttcaagactctgtttccaccattccagaagtacatcaccaagggttacgtgtcggaagaagaagctggtcgtcgtctggcagcagtcatctctgatcctaagctgaacaagtctggtgcatactggtcttggtcttccaccactgggtcgttcgacaacaagtgtcttaagaagtggccgaagaatccaaggcctcccacctgtgggaaatctctgccaagctggtgggcctgagcgcgtaataataatactagagagctttaagaaggagatatacataatggcaatggcagcatctcgtcaggctgttcgcgttgcagcagctgtggatgctgattaccgtaagcgcgaaccaaaggacgtgcgcgtgcttgttgtaggtccaacaggttacatcggtaagttcgtggtgaaggaactcgtgagccgtggttacaacgtggttgcattcgcacgtgagaatgcaggtatcaagggtaagatgggtcgtgaagatatcgtgaaggagttccatggtgcggaagtgcgtttcggttctgtgctggatccagcttcgctgcgtgacgttgcattcaaggacccagttgacgttgttgttagctgcctggcaagccgcacaggtggtaagaaggactcgtggctgatcgactacacagctactaagaacagcctggacgttgcacgcgcatctggtgcaaagcactttgtgctgctgtctgcaatctgcgtgcagaagccactgctggagttccagaaagccaagctccagttcgagtctgaccttcaggctgccggtgacatcacctattccatcgtgcgtcctaccgcattcttcaagtccattgctggtcagatcgacatcgtgaagaaaggtaacccatacgtcatgtttggtgacggtaacctggcagcatgcaaacctatcagcgaagctgacctggcttcattcattgcagattgcgtcaccgaacagaacaaggtcaacaaggtgctgcctatcggtggtccaagcaaggccttcacggctaagcagcaggctgatctgctgttcaacatcacaggtctgccacctaagtacttccctgtgcctgtggcactcatggatggtatgattggtctgttcgactctctggctaagctgttcccacagctggaagatagcgctgaatttgcacgtattggtaagtactatgccaccgaatccatgctggtgtacgacgaggcacgtggtgtgtaccggaagacgaaacgcctggttacggcaaggacacgctggaagacttcttctctcgtgcagtgaaggaaggtctgcaaggacaggaactgggtgaccaggcagtgtttggacaacaataataataatactagagagctttaagaaggagatatacataatggtcatcggtggtggtccatctggtgcatgcgcagcagagaccctggctaagggtggtgtggaaaccttcctgctggaacgcaagctggacaactgcaagccatgcggtggtgccatccctctgtgcatggttgaagagttcgacctgcctatggagattattgaccgtcgcgtcaccaagatgaagatgatctctccttccaaccgtgaagtcgatgtgggtaagacactcagcgaaacagagtggattggtatgtgccgtcgtgaagtgtttgatgactacctgcgtaaccgtgcacagaagctgggtgctaacatcgtgaacggtctgttcatgcgttctgagcagcagagcgctgaaggtcctttcaccatccactacaacagctacgaggatggtagcaagatgggtaagcctgctacactggaagtcgacatgatcatcggtgctgacggtgcaaacagccgtatcgccaaggagatcgacgctggcgagtacgactacgcaattgcattccaggaacgtatccgtatcccagatgacaagatgaagtactatgagaacctggctgagatgtacgttggtgacgacgtgtctccagacttctacggatgggtgttccctaagtacgaccacgttgcagttggtacaggtacagttgtcaacaagacagctatcaagcagtaccagcaggctacacgtgatcgctctaaggtcaagaccgaaggtggtaagatcatccgtgttgaggcacatccaatcccagagcatccacgtccacgtcgttgcaagggtcgtgttgcactggtcggtgacgctgctggttacgtgaccaagtgctctggtgaaggtatctacttcgctgctaagtctggtcgtatggcagctgaagctattgtggaaggttctgctaacggaactaagatgtgcggtgaagatgcaatccgtgtctacctggacaaatgggatcgtaagtactggacaacatacaaggttctggacatcctgcaaaaggtttt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igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z