BBa_K1640024 1 BBa_K1640024 psbC 2015-09-17T11:00:00Z 2015-09-18T12:20:25Z n/a n/a false false _2057_ 16353 28565 9 false n/a false Meghan Cook annotation2469608 1 RBS range2469608 1 1 19 annotation2469609 1 psbC range2469609 1 20 1405 BBa_K1998003 1 BBa_K1998003 psbD 2016-10-11T11:00:00Z 2016-10-17T05:17:58Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. The psbD gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core. false false _2465_ 33907 33907 9 false Sequence had to be adjusted to allow for 1 bp correction to the BBa suffix ﴾G>C﴿ so that the SpeI cut site is intact. false Shauna Winchester annotation2515669 1 Start range2515669 1 61 64 annotation2514098 1 psbD range2514098 1 65 1210 annotation2514097 1 RBS range2514097 1 46 60 annotation2514096 1 Plac range2514096 1 7 45 BBa_K1998014 1 BBa_K1998014 psbDCA 2016-10-12T11:00:00Z 2016-10-17T01:27:57Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. This part is composed of psbD, psbC and psbA. These parts make up one of the operons in the photosystem II pathway. The psbD gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core. This part is made up of psbC and psbA. The psbC gene that comprises this part codes a subunit that makes up the Photosystem II protein P680. The P680 complex is photosystem II primary electron donor. The psbA gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core. false false _2465_ 33907 33907 9 false Codon optimisation false Shauna Winchester component2514122 1 BBa_K1998005 component2514114 1 BBa_K1998003 annotation2514114 1 BBa_K1998003 range2514114 1 1 1210 annotation2514122 1 BBa_K1998005 range2514122 1 1219 3834 BBa_K1998005 1 BBa_K1998005 psbCA 2016-10-11T11:00:00Z 2016-10-12T05:37:32Z The genes were sourced from Chlamydomonas rienhardtii and synthesised. This part is made up of psbC and psbA. The psbC gene that comprises this part codes a subunit that makes up the Photosystem II protein P680. The P680 complex is photosystem II primary electron donor. The psbA gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core. The psbD gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core. false false _2465_ 33907 33907 9 false Genes were codon optimised for bacteria using optimiser tool Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence. false Shauna Winchester component2495376 1 BBa_K1640020 component2495372 1 BBa_K1640024 annotation2495372 1 BBa_K1640024 range2495372 1 1 1405 annotation2495376 1 BBa_K1640020 range2495376 1 1414 2616 BBa_K1640020 1 BBa_K1640020 psbA 2015-09-09T11:00:00Z 2015-09-18T08:54:57Z n/a n/a false false _2057_ 28565 28565 9 true n/a false Meghan Cook annotation2448252 1 psbA range2448252 1 16 1074 annotation2448253 1 double terminator range2448253 1 1075 1203 annotation2448251 1 rbs range2448251 1 1 15 BBa_K1640024_sequence 1 agaagaaggagatatacatatggagacactgtttaatggtacactgacggtcggcggccgtgaccaggaaaccactgggtttgcttggtggtcggggaatgctcgcttaatcaatctgagcgggaaattactgggtgctcatgtagctcacgcaggcctgatcgttttttgggccggtgcaatgaatttgttcgaagtgtcacatttcgttccggaaaagcccatgtatgagcagggcctgattctgttaccccacattgcgacactgggctacggggtgggtccgggcggcgagatcattgatactttcccgtactttgtaagcggtgtgctgcatctgatttcgtctgccgtgctcggcttcggcggcgtttatcacagtctgattgggccggaaaccctggaagaatcatatccatttttcggctacgtgtggaaagataaaaacaaaatgacgaatattttgggttaccatctgattatgctgggtctgggagcttggctcctggtgtggaaggccatgtatttcggcggtgtgtatgatacatgggcaccgggcggcggcgatgtccgcgttattacaaacccgaccacgaatgcggcagtcatcttcggttatctggtcaaatctccgtttggaggtgacggttggatttgttctgtggataatatggaggatattatcgggggccacatctggatcggcaccttagaaattctgggtggcatctggcatatttacacgacaccttggccctgggcgcgccgcgcttttgtttggtccggagaagcctacctgtcatatagtctgggtgcaatcggcgttatggggtttatcgcgtgttgtatgagttggttcaataatactgcctatccaagcgaattttatggaccaacgggacccgaagcgagtcaatctcaagcgttcacctttctggtccgtgaccaacgcttaggcgccaatgtggcgagcgcccagggcccgaccggacttgggaaatacctgatgcgctctccaacaggtgagatcattttcggcggtgagaccatgcggttctgggactttcgtggcccgtggttggaaccgctgcgtggtcctaacggactggatctgaacaaactgaaaaacgatattcagccctggcaagaacgtcgggcggcggagtatatgacgcacgctccactcggcagcctgaactcggtcggtggtgtcgctactgaaatcaacgcggtgaatttcgtgtcgccacgctcatggctggcgtgctctcatttttgcctgggcttcttcttttttattggacatctgtggcatgcgggccgcgcgcgtgcagcggcagcagggttcgagaaaggtatcgaccgcttcgatgagccagttctgagcatgcgccctctggactaa BBa_K1998003_sequence 1 ctagagggctttacactttatgcttccggctcgtatgttgtgtggaaggaggtaaaaaaatgacaattgcgatcggtacatatcaagagaaacgcacatggttcgatgacgctgatgactggcttcgtcaagaccgtttcgtattcgtaggttggtcaggtttattactattcccttgtgcttactttgcattaggtggttggttaactggtactactttcgttacttcatggtatacgcatggtttagctacttcttacttagaaggttgtaacttcttaacagcagctgtttctacacctgctaacagtatggctcactctcttctatttgtttggggtccagaagctcaaggtgatttcactcgttggtgtcaacttggtggtttatgggcattcgttgctttacacggtgcatttggtttaattggtttcatgcttcgtcagtttgaaattgctcgttcagtaaacttacgtccatacaacgcaattgctttctcagcaccaattgctgtattcgtttcagtattcctaatttacccattaggtcaatcaggttggttctttgcacctagtttcggtgtagctgctatcttccgtttcattttattcttccaaggtttccacaactggacacttaacccattccacatgatgggtgttgctggtgttttaggtgctgctttattatgtgctattcacggtgctactgttgaaaacacattattcgaagacggtgacggtgctaacacattccgtgcattcaaccctacacaggctgaagaaacatactctatggttactgctaaccgtttctggtcacaaatcttcggtgttgctttctctaacaaacgttggcttcacttcttcatgttattagttccagtaactggtctttggatgagtgctattggtgttgtaggtttagctctaaacttacgtgcttacgacttcgtatcacaaaagattcgtgctgctgaaaaaccctgaatttgaaacattctacactaaaaaacattcttcttaacgaaaggtattcctggcttggaagggctgcttcagaaccaaccacacgaaacgtttagtattttcttgaaaaaaaatttaccaccgggggaaaccctcttatataacaaataagcggcgccttgcaatcccgggcaaaaaaagggcaagggggtcccacccccgggcccttttttttcaaaaag BBa_K1640020_sequence 1 agaggaggtaaaaaaatgacagcaattttagaacgtcgtgaaaattctagcctatgggctcgtttttgtgagtggatcacttcaactgaaaaccgtttatacatcggttggttcggtgtaatcatgatcccatgtcttcttactgcaacatcagtattcatcatcgctttcatcgctgctccgccagtagacatcgatggtatccgtgaaccagtttcaggttctcttctttacggtaacaacatcattacaggtgctgtaatcccaacttctaacgcaatcggtcttcacttctacccaatttgggaagctgcttccctagacgagtggttatacaacggtggtccttaccaacttatcgtttgtcacttccttctaggtgtatactgctacatgggtcgtgagtgggaattatctttccgtttaggtatgcgtccatggatcgctgtagcttactcagctccagtagcagcagcttcagctgtattcttagtttaccctatcggccaaggttcattctctgacggtatgcctttaggtatctctggtactttcaacttcatgatcgtattccaagcagaacacaacatccttatgcacccattccacatgttaggtgttgctggtgtattcggtggttcattattctcagctatgcacggttctttagttacttcatctttaatccgtgaaacaactgaaaacgaatcagctaacgaaggttaccgtttcggtcaagaagaagaaacttacaacattgtagctgctcatggttactttggtcgtctaatcttccaatacgcttctttcaacaactctcgttcattacacttcttcttagctgcttggccggtaatcggtatttggttcactgctttaggtttatcaactatggcattcaacttaaacggtttcaacttcaaccaatcagtagtagactcacaaggtcgtgtactaaacacttgggcagacatcatcaaccgtgctaacttaggtatggaagtaatgcacgagcgtaacgctcacaacttcccactagacttagcttcaactaactctagctcaaacaactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1998005_sequence 1 agaagaaggagatatacatatggagacactgtttaatggtacactgacggtcggcggccgtgaccaggaaaccactgggtttgcttggtggtcggggaatgctcgcttaatcaatctgagcgggaaattactgggtgctcatgtagctcacgcaggcctgatcgttttttgggccggtgcaatgaatttgttcgaagtgtcacatttcgttccggaaaagcccatgtatgagcagggcctgattctgttaccccacattgcgacactgggctacggggtgggtccgggcggcgagatcattgatactttcccgtactttgtaagcggtgtgctgcatctgatttcgtctgccgtgctcggcttcggcggcgtttatcacagtctgattgggccggaaaccctggaagaatcatatccatttttcggctacgtgtggaaagataaaaacaaaatgacgaatattttgggttaccatctgattatgctgggtctgggagcttggctcctggtgtggaaggccatgtatttcggcggtgtgtatgatacatgggcaccgggcggcggcgatgtccgcgttattacaaacccgaccacgaatgcggcagtcatcttcggttatctggtcaaatctccgtttggaggtgacggttggatttgttctgtggataatatggaggatattatcgggggccacatctggatcggcaccttagaaattctgggtggcatctggcatatttacacgacaccttggccctgggcgcgccgcgcttttgtttggtccggagaagcctacctgtcatatagtctgggtgcaatcggcgttatggggtttatcgcgtgttgtatgagttggttcaataatactgcctatccaagcgaattttatggaccaacgggacccgaagcgagtcaatctcaagcgttcacctttctggtccgtgaccaacgcttaggcgccaatgtggcgagcgcccagggcccgaccggacttgggaaatacctgatgcgctctccaacaggtgagatcattttcggcggtgagaccatgcggttctgggactttcgtggcccgtggttggaaccgctgcgtggtcctaacggactggatctgaacaaactgaaaaacgatattcagccctggcaagaacgtcgggcggcggagtatatgacgcacgctccactcggcagcctgaactcggtcggtggtgtcgctactgaaatcaacgcggtgaatttcgtgtcgccacgctcatggctggcgtgctctcatttttgcctgggcttcttcttttttattggacatctgtggcatgcgggccgcgcgcgtgcagcggcagcagggttcgagaaaggtatcgaccgcttcgatgagccagttctgagcatgcgccctctggactaatactagagagaggaggtaaaaaaatgacagcaattttagaacgtcgtgaaaattctagcctatgggctcgtttttgtgagtggatcacttcaactgaaaaccgtttatacatcggttggttcggtgtaatcatgatcccatgtcttcttactgcaacatcagtattcatcatcgctttcatcgctgctccgccagtagacatcgatggtatccgtgaaccagtttcaggttctcttctttacggtaacaacatcattacaggtgctgtaatcccaacttctaacgcaatcggtcttcacttctacccaatttgggaagctgcttccctagacgagtggttatacaacggtggtccttaccaacttatcgtttgtcacttccttctaggtgtatactgctacatgggtcgtgagtgggaattatctttccgtttaggtatgcgtccatggatcgctgtagcttactcagctccagtagcagcagcttcagctgtattcttagtttaccctatcggccaaggttcattctctgacggtatgcctttaggtatctctggtactttcaacttcatgatcgtattccaagcagaacacaacatccttatgcacccattccacatgttaggtgttgctggtgtattcggtggttcattattctcagctatgcacggttctttagttacttcatctttaatccgtgaaacaactgaaaacgaatcagctaacgaaggttaccgtttcggtcaagaagaagaaacttacaacattgtagctgctcatggttactttggtcgtctaatcttccaatacgcttctttcaacaactctcgttcattacacttcttcttagctgcttggccggtaatcggtatttggttcactgctttaggtttatcaactatggcattcaacttaaacggtttcaacttcaaccaatcagtagtagactcacaaggtcgtgtactaaacacttgggcagacatcatcaaccgtgctaacttaggtatggaagtaatgcacgagcgtaacgctcacaacttcccactagacttagcttcaactaactctagctcaaacaactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1998014_sequence 1 ctagagggctttacactttatgcttccggctcgtatgttgtgtggaaggaggtaaaaaaatgacaattgcgatcggtacatatcaagagaaacgcacatggttcgatgacgctgatgactggcttcgtcaagaccgtttcgtattcgtaggttggtcaggtttattactattcccttgtgcttactttgcattaggtggttggttaactggtactactttcgttacttcatggtatacgcatggtttagctacttcttacttagaaggttgtaacttcttaacagcagctgtttctacacctgctaacagtatggctcactctcttctatttgtttggggtccagaagctcaaggtgatttcactcgttggtgtcaacttggtggtttatgggcattcgttgctttacacggtgcatttggtttaattggtttcatgcttcgtcagtttgaaattgctcgttcagtaaacttacgtccatacaacgcaattgctttctcagcaccaattgctgtattcgtttcagtattcctaatttacccattaggtcaatcaggttggttctttgcacctagtttcggtgtagctgctatcttccgtttcattttattcttccaaggtttccacaactggacacttaacccattccacatgatgggtgttgctggtgttttaggtgctgctttattatgtgctattcacggtgctactgttgaaaacacattattcgaagacggtgacggtgctaacacattccgtgcattcaaccctacacaggctgaagaaacatactctatggttactgctaaccgtttctggtcacaaatcttcggtgttgctttctctaacaaacgttggcttcacttcttcatgttattagttccagtaactggtctttggatgagtgctattggtgttgtaggtttagctctaaacttacgtgcttacgacttcgtatcacaaaagattcgtgctgctgaaaaaccctgaatttgaaacattctacactaaaaaacattcttcttaacgaaaggtattcctggcttggaagggctgcttcagaaccaaccacacgaaacgtttagtattttcttgaaaaaaaatttaccaccgggggaaaccctcttatataacaaataagcggcgccttgcaatcccgggcaaaaaaagggcaagggggtcccacccccgggcccttttttttcaaaaagtactagagagaagaaggagatatacatatggagacactgtttaatggtacactgacggtcggcggccgtgaccaggaaaccactgggtttgcttggtggtcggggaatgctcgcttaatcaatctgagcgggaaattactgggtgctcatgtagctcacgcaggcctgatcgttttttgggccggtgcaatgaatttgttcgaagtgtcacatttcgttccggaaaagcccatgtatgagcagggcctgattctgttaccccacattgcgacactgggctacggggtgggtccgggcggcgagatcattgatactttcccgtactttgtaagcggtgtgctgcatctgatttcgtctgccgtgctcggcttcggcggcgtttatcacagtctgattgggccggaaaccctggaagaatcatatccatttttcggctacgtgtggaaagataaaaacaaaatgacgaatattttgggttaccatctgattatgctgggtctgggagcttggctcctggtgtggaaggccatgtatttcggcggtgtgtatgatacatgggcaccgggcggcggcgatgtccgcgttattacaaacccgaccacgaatgcggcagtcatcttcggttatctggtcaaatctccgtttggaggtgacggttggatttgttctgtggataatatggaggatattatcgggggccacatctggatcggcaccttagaaattctgggtggcatctggcatatttacacgacaccttggccctgggcgcgccgcgcttttgtttggtccggagaagcctacctgtcatatagtctgggtgcaatcggcgttatggggtttatcgcgtgttgtatgagttggttcaataatactgcctatccaagcgaattttatggaccaacgggacccgaagcgagtcaatctcaagcgttcacctttctggtccgtgaccaacgcttaggcgccaatgtggcgagcgcccagggcccgaccggacttgggaaatacctgatgcgctctccaacaggtgagatcattttcggcggtgagaccatgcggttctgggactttcgtggcccgtggttggaaccgctgcgtggtcctaacggactggatctgaacaaactgaaaaacgatattcagccctggcaagaacgtcgggcggcggagtatatgacgcacgctccactcggcagcctgaactcggtcggtggtgtcgctactgaaatcaacgcggtgaatttcgtgtcgccacgctcatggctggcgtgctctcatttttgcctgggcttcttcttttttattggacatctgtggcatgcgggccgcgcgcgtgcagcggcagcagggttcgagaaaggtatcgaccgcttcgatgagccagttctgagcatgcgccctctggactaatactagagagaggaggtaaaaaaatgacagcaattttagaacgtcgtgaaaattctagcctatgggctcgtttttgtgagtggatcacttcaactgaaaaccgtttatacatcggttggttcggtgtaatcatgatcccatgtcttcttactgcaacatcagtattcatcatcgctttcatcgctgctccgccagtagacatcgatggtatccgtgaaccagtttcaggttctcttctttacggtaacaacatcattacaggtgctgtaatcccaacttctaacgcaatcggtcttcacttctacccaatttgggaagctgcttccctagacgagtggttatacaacggtggtccttaccaacttatcgtttgtcacttccttctaggtgtatactgctacatgggtcgtgagtgggaattatctttccgtttaggtatgcgtccatggatcgctgtagcttactcagctccagtagcagcagcttcagctgtattcttagtttaccctatcggccaaggttcattctctgacggtatgcctttaggtatctctggtactttcaacttcatgatcgtattccaagcagaacacaacatccttatgcacccattccacatgttaggtgttgctggtgtattcggtggttcattattctcagctatgcacggttctttagttacttcatctttaatccgtgaaacaactgaaaacgaatcagctaacgaaggttaccgtttcggtcaagaagaagaaacttacaacattgtagctgctcatggttactttggtcgtctaatcttccaatacgcttctttcaacaactctcgttcattacacttcttcttagctgcttggccggtaatcggtatttggttcactgctttaggtttatcaactatggcattcaacttaaacggtttcaacttcaaccaatcagtagtagactcacaaggtcgtgtactaaacacttgggcagacatcatcaaccgtgctaacttaggtatggaagtaatgcacgagcgtaacgctcacaacttcccactagacttagcttcaactaactctagctcaaacaactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z