BBa_K1998014
1
BBa_K1998014
psbDCA
2016-10-12T11:00:00Z
2016-10-17T01:27:57Z
The genes were sourced from Chlamydomonas rienhardtii and synthesised.
This part is composed of psbD, psbC and psbA. These parts make up one of the operons in the photosystem II pathway. The psbD gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core. This part is made up of psbC and psbA. The psbC gene that comprises this part codes a subunit that makes up the Photosystem II protein P680. The P680 complex is photosystem II primary electron donor. The psbA gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core.
false
false
_2465_
33907
33907
9
false
Codon optimisation
false
Shauna Winchester
component2514114
1
BBa_K1998003
component2514122
1
BBa_K1998005
annotation2514114
1
BBa_K1998003
range2514114
1
1
1210
annotation2514122
1
BBa_K1998005
range2514122
1
1219
3834
BBa_K1998003
1
BBa_K1998003
psbD
2016-10-11T11:00:00Z
2016-10-17T05:17:58Z
The genes were sourced from Chlamydomonas rienhardtii and synthesised.
The psbD gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core.
false
false
_2465_
33907
33907
9
false
Sequence had to be adjusted to allow for 1 bp correction to the BBa suffix ﴾G>C﴿ so that the SpeI cut site is intact.
false
Shauna Winchester
annotation2514096
1
Plac
range2514096
1
7
45
annotation2514097
1
RBS
range2514097
1
46
60
annotation2514098
1
psbD
range2514098
1
65
1210
annotation2515669
1
Start
range2515669
1
61
64
BBa_K1640024
1
BBa_K1640024
psbC
2015-09-17T11:00:00Z
2015-09-18T12:20:25Z
n/a
n/a
false
false
_2057_
16353
28565
9
false
n/a
false
Meghan Cook
annotation2469608
1
RBS
range2469608
1
1
19
annotation2469609
1
psbC
range2469609
1
20
1405
BBa_K1998005
1
BBa_K1998005
psbCA
2016-10-11T11:00:00Z
2016-10-12T05:37:32Z
The genes were sourced from Chlamydomonas rienhardtii and synthesised.
This part is made up of psbC and psbA. The psbC gene that comprises this part codes a subunit that makes up the Photosystem II protein P680. The P680 complex is photosystem II primary electron donor. The psbA gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core. The psbD gene that comprises this part encodes the D2 protein, which is one of the two proteins that comprise the Photosystem II reaction center core.
false
false
_2465_
33907
33907
9
false
Genes were codon optimised for bacteria using optimiser tool
Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence.
false
Shauna Winchester
component2495376
1
BBa_K1640020
component2495372
1
BBa_K1640024
annotation2495376
1
BBa_K1640020
range2495376
1
1414
2616
annotation2495372
1
BBa_K1640024
range2495372
1
1
1405
BBa_K1640020
1
BBa_K1640020
psbA
2015-09-09T11:00:00Z
2015-09-18T08:54:57Z
n/a
n/a
false
false
_2057_
28565
28565
9
true
n/a
false
Meghan Cook
annotation2448253
1
double terminator
range2448253
1
1075
1203
annotation2448252
1
psbA
range2448252
1
16
1074
annotation2448251
1
rbs
range2448251
1
1
15
BBa_K1640024_sequence
1
agaagaaggagatatacatatggagacactgtttaatggtacactgacggtcggcggccgtgaccaggaaaccactgggtttgcttggtggtcggggaatgctcgcttaatcaatctgagcgggaaattactgggtgctcatgtagctcacgcaggcctgatcgttttttgggccggtgcaatgaatttgttcgaagtgtcacatttcgttccggaaaagcccatgtatgagcagggcctgattctgttaccccacattgcgacactgggctacggggtgggtccgggcggcgagatcattgatactttcccgtactttgtaagcggtgtgctgcatctgatttcgtctgccgtgctcggcttcggcggcgtttatcacagtctgattgggccggaaaccctggaagaatcatatccatttttcggctacgtgtggaaagataaaaacaaaatgacgaatattttgggttaccatctgattatgctgggtctgggagcttggctcctggtgtggaaggccatgtatttcggcggtgtgtatgatacatgggcaccgggcggcggcgatgtccgcgttattacaaacccgaccacgaatgcggcagtcatcttcggttatctggtcaaatctccgtttggaggtgacggttggatttgttctgtggataatatggaggatattatcgggggccacatctggatcggcaccttagaaattctgggtggcatctggcatatttacacgacaccttggccctgggcgcgccgcgcttttgtttggtccggagaagcctacctgtcatatagtctgggtgcaatcggcgttatggggtttatcgcgtgttgtatgagttggttcaataatactgcctatccaagcgaattttatggaccaacgggacccgaagcgagtcaatctcaagcgttcacctttctggtccgtgaccaacgcttaggcgccaatgtggcgagcgcccagggcccgaccggacttgggaaatacctgatgcgctctccaacaggtgagatcattttcggcggtgagaccatgcggttctgggactttcgtggcccgtggttggaaccgctgcgtggtcctaacggactggatctgaacaaactgaaaaacgatattcagccctggcaagaacgtcgggcggcggagtatatgacgcacgctccactcggcagcctgaactcggtcggtggtgtcgctactgaaatcaacgcggtgaatttcgtgtcgccacgctcatggctggcgtgctctcatttttgcctgggcttcttcttttttattggacatctgtggcatgcgggccgcgcgcgtgcagcggcagcagggttcgagaaaggtatcgaccgcttcgatgagccagttctgagcatgcgccctctggactaa
BBa_K1998003_sequence
1
ctagagggctttacactttatgcttccggctcgtatgttgtgtggaaggaggtaaaaaaatgacaattgcgatcggtacatatcaagagaaacgcacatggttcgatgacgctgatgactggcttcgtcaagaccgtttcgtattcgtaggttggtcaggtttattactattcccttgtgcttactttgcattaggtggttggttaactggtactactttcgttacttcatggtatacgcatggtttagctacttcttacttagaaggttgtaacttcttaacagcagctgtttctacacctgctaacagtatggctcactctcttctatttgtttggggtccagaagctcaaggtgatttcactcgttggtgtcaacttggtggtttatgggcattcgttgctttacacggtgcatttggtttaattggtttcatgcttcgtcagtttgaaattgctcgttcagtaaacttacgtccatacaacgcaattgctttctcagcaccaattgctgtattcgtttcagtattcctaatttacccattaggtcaatcaggttggttctttgcacctagtttcggtgtagctgctatcttccgtttcattttattcttccaaggtttccacaactggacacttaacccattccacatgatgggtgttgctggtgttttaggtgctgctttattatgtgctattcacggtgctactgttgaaaacacattattcgaagacggtgacggtgctaacacattccgtgcattcaaccctacacaggctgaagaaacatactctatggttactgctaaccgtttctggtcacaaatcttcggtgttgctttctctaacaaacgttggcttcacttcttcatgttattagttccagtaactggtctttggatgagtgctattggtgttgtaggtttagctctaaacttacgtgcttacgacttcgtatcacaaaagattcgtgctgctgaaaaaccctgaatttgaaacattctacactaaaaaacattcttcttaacgaaaggtattcctggcttggaagggctgcttcagaaccaaccacacgaaacgtttagtattttcttgaaaaaaaatttaccaccgggggaaaccctcttatataacaaataagcggcgccttgcaatcccgggcaaaaaaagggcaagggggtcccacccccgggcccttttttttcaaaaag
BBa_K1640020_sequence
1
agaggaggtaaaaaaatgacagcaattttagaacgtcgtgaaaattctagcctatgggctcgtttttgtgagtggatcacttcaactgaaaaccgtttatacatcggttggttcggtgtaatcatgatcccatgtcttcttactgcaacatcagtattcatcatcgctttcatcgctgctccgccagtagacatcgatggtatccgtgaaccagtttcaggttctcttctttacggtaacaacatcattacaggtgctgtaatcccaacttctaacgcaatcggtcttcacttctacccaatttgggaagctgcttccctagacgagtggttatacaacggtggtccttaccaacttatcgtttgtcacttccttctaggtgtatactgctacatgggtcgtgagtgggaattatctttccgtttaggtatgcgtccatggatcgctgtagcttactcagctccagtagcagcagcttcagctgtattcttagtttaccctatcggccaaggttcattctctgacggtatgcctttaggtatctctggtactttcaacttcatgatcgtattccaagcagaacacaacatccttatgcacccattccacatgttaggtgttgctggtgtattcggtggttcattattctcagctatgcacggttctttagttacttcatctttaatccgtgaaacaactgaaaacgaatcagctaacgaaggttaccgtttcggtcaagaagaagaaacttacaacattgtagctgctcatggttactttggtcgtctaatcttccaatacgcttctttcaacaactctcgttcattacacttcttcttagctgcttggccggtaatcggtatttggttcactgctttaggtttatcaactatggcattcaacttaaacggtttcaacttcaaccaatcagtagtagactcacaaggtcgtgtactaaacacttgggcagacatcatcaaccgtgctaacttaggtatggaagtaatgcacgagcgtaacgctcacaacttcccactagacttagcttcaactaactctagctcaaacaactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1998005_sequence
1
agaagaaggagatatacatatggagacactgtttaatggtacactgacggtcggcggccgtgaccaggaaaccactgggtttgcttggtggtcggggaatgctcgcttaatcaatctgagcgggaaattactgggtgctcatgtagctcacgcaggcctgatcgttttttgggccggtgcaatgaatttgttcgaagtgtcacatttcgttccggaaaagcccatgtatgagcagggcctgattctgttaccccacattgcgacactgggctacggggtgggtccgggcggcgagatcattgatactttcccgtactttgtaagcggtgtgctgcatctgatttcgtctgccgtgctcggcttcggcggcgtttatcacagtctgattgggccggaaaccctggaagaatcatatccatttttcggctacgtgtggaaagataaaaacaaaatgacgaatattttgggttaccatctgattatgctgggtctgggagcttggctcctggtgtggaaggccatgtatttcggcggtgtgtatgatacatgggcaccgggcggcggcgatgtccgcgttattacaaacccgaccacgaatgcggcagtcatcttcggttatctggtcaaatctccgtttggaggtgacggttggatttgttctgtggataatatggaggatattatcgggggccacatctggatcggcaccttagaaattctgggtggcatctggcatatttacacgacaccttggccctgggcgcgccgcgcttttgtttggtccggagaagcctacctgtcatatagtctgggtgcaatcggcgttatggggtttatcgcgtgttgtatgagttggttcaataatactgcctatccaagcgaattttatggaccaacgggacccgaagcgagtcaatctcaagcgttcacctttctggtccgtgaccaacgcttaggcgccaatgtggcgagcgcccagggcccgaccggacttgggaaatacctgatgcgctctccaacaggtgagatcattttcggcggtgagaccatgcggttctgggactttcgtggcccgtggttggaaccgctgcgtggtcctaacggactggatctgaacaaactgaaaaacgatattcagccctggcaagaacgtcgggcggcggagtatatgacgcacgctccactcggcagcctgaactcggtcggtggtgtcgctactgaaatcaacgcggtgaatttcgtgtcgccacgctcatggctggcgtgctctcatttttgcctgggcttcttcttttttattggacatctgtggcatgcgggccgcgcgcgtgcagcggcagcagggttcgagaaaggtatcgaccgcttcgatgagccagttctgagcatgcgccctctggactaatactagagagaggaggtaaaaaaatgacagcaattttagaacgtcgtgaaaattctagcctatgggctcgtttttgtgagtggatcacttcaactgaaaaccgtttatacatcggttggttcggtgtaatcatgatcccatgtcttcttactgcaacatcagtattcatcatcgctttcatcgctgctccgccagtagacatcgatggtatccgtgaaccagtttcaggttctcttctttacggtaacaacatcattacaggtgctgtaatcccaacttctaacgcaatcggtcttcacttctacccaatttgggaagctgcttccctagacgagtggttatacaacggtggtccttaccaacttatcgtttgtcacttccttctaggtgtatactgctacatgggtcgtgagtgggaattatctttccgtttaggtatgcgtccatggatcgctgtagcttactcagctccagtagcagcagcttcagctgtattcttagtttaccctatcggccaaggttcattctctgacggtatgcctttaggtatctctggtactttcaacttcatgatcgtattccaagcagaacacaacatccttatgcacccattccacatgttaggtgttgctggtgtattcggtggttcattattctcagctatgcacggttctttagttacttcatctttaatccgtgaaacaactgaaaacgaatcagctaacgaaggttaccgtttcggtcaagaagaagaaacttacaacattgtagctgctcatggttactttggtcgtctaatcttccaatacgcttctttcaacaactctcgttcattacacttcttcttagctgcttggccggtaatcggtatttggttcactgctttaggtttatcaactatggcattcaacttaaacggtttcaacttcaaccaatcagtagtagactcacaaggtcgtgtactaaacacttgggcagacatcatcaaccgtgctaacttaggtatggaagtaatgcacgagcgtaacgctcacaacttcccactagacttagcttcaactaactctagctcaaacaactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1998014_sequence
1
ctagagggctttacactttatgcttccggctcgtatgttgtgtggaaggaggtaaaaaaatgacaattgcgatcggtacatatcaagagaaacgcacatggttcgatgacgctgatgactggcttcgtcaagaccgtttcgtattcgtaggttggtcaggtttattactattcccttgtgcttactttgcattaggtggttggttaactggtactactttcgttacttcatggtatacgcatggtttagctacttcttacttagaaggttgtaacttcttaacagcagctgtttctacacctgctaacagtatggctcactctcttctatttgtttggggtccagaagctcaaggtgatttcactcgttggtgtcaacttggtggtttatgggcattcgttgctttacacggtgcatttggtttaattggtttcatgcttcgtcagtttgaaattgctcgttcagtaaacttacgtccatacaacgcaattgctttctcagcaccaattgctgtattcgtttcagtattcctaatttacccattaggtcaatcaggttggttctttgcacctagtttcggtgtagctgctatcttccgtttcattttattcttccaaggtttccacaactggacacttaacccattccacatgatgggtgttgctggtgttttaggtgctgctttattatgtgctattcacggtgctactgttgaaaacacattattcgaagacggtgacggtgctaacacattccgtgcattcaaccctacacaggctgaagaaacatactctatggttactgctaaccgtttctggtcacaaatcttcggtgttgctttctctaacaaacgttggcttcacttcttcatgttattagttccagtaactggtctttggatgagtgctattggtgttgtaggtttagctctaaacttacgtgcttacgacttcgtatcacaaaagattcgtgctgctgaaaaaccctgaatttgaaacattctacactaaaaaacattcttcttaacgaaaggtattcctggcttggaagggctgcttcagaaccaaccacacgaaacgtttagtattttcttgaaaaaaaatttaccaccgggggaaaccctcttatataacaaataagcggcgccttgcaatcccgggcaaaaaaagggcaagggggtcccacccccgggcccttttttttcaaaaagtactagagagaagaaggagatatacatatggagacactgtttaatggtacactgacggtcggcggccgtgaccaggaaaccactgggtttgcttggtggtcggggaatgctcgcttaatcaatctgagcgggaaattactgggtgctcatgtagctcacgcaggcctgatcgttttttgggccggtgcaatgaatttgttcgaagtgtcacatttcgttccggaaaagcccatgtatgagcagggcctgattctgttaccccacattgcgacactgggctacggggtgggtccgggcggcgagatcattgatactttcccgtactttgtaagcggtgtgctgcatctgatttcgtctgccgtgctcggcttcggcggcgtttatcacagtctgattgggccggaaaccctggaagaatcatatccatttttcggctacgtgtggaaagataaaaacaaaatgacgaatattttgggttaccatctgattatgctgggtctgggagcttggctcctggtgtggaaggccatgtatttcggcggtgtgtatgatacatgggcaccgggcggcggcgatgtccgcgttattacaaacccgaccacgaatgcggcagtcatcttcggttatctggtcaaatctccgtttggaggtgacggttggatttgttctgtggataatatggaggatattatcgggggccacatctggatcggcaccttagaaattctgggtggcatctggcatatttacacgacaccttggccctgggcgcgccgcgcttttgtttggtccggagaagcctacctgtcatatagtctgggtgcaatcggcgttatggggtttatcgcgtgttgtatgagttggttcaataatactgcctatccaagcgaattttatggaccaacgggacccgaagcgagtcaatctcaagcgttcacctttctggtccgtgaccaacgcttaggcgccaatgtggcgagcgcccagggcccgaccggacttgggaaatacctgatgcgctctccaacaggtgagatcattttcggcggtgagaccatgcggttctgggactttcgtggcccgtggttggaaccgctgcgtggtcctaacggactggatctgaacaaactgaaaaacgatattcagccctggcaagaacgtcgggcggcggagtatatgacgcacgctccactcggcagcctgaactcggtcggtggtgtcgctactgaaatcaacgcggtgaatttcgtgtcgccacgctcatggctggcgtgctctcatttttgcctgggcttcttcttttttattggacatctgtggcatgcgggccgcgcgcgtgcagcggcagcagggttcgagaaaggtatcgaccgcttcgatgagccagttctgagcatgcgccctctggactaatactagagagaggaggtaaaaaaatgacagcaattttagaacgtcgtgaaaattctagcctatgggctcgtttttgtgagtggatcacttcaactgaaaaccgtttatacatcggttggttcggtgtaatcatgatcccatgtcttcttactgcaacatcagtattcatcatcgctttcatcgctgctccgccagtagacatcgatggtatccgtgaaccagtttcaggttctcttctttacggtaacaacatcattacaggtgctgtaatcccaacttctaacgcaatcggtcttcacttctacccaatttgggaagctgcttccctagacgagtggttatacaacggtggtccttaccaacttatcgtttgtcacttccttctaggtgtatactgctacatgggtcgtgagtgggaattatctttccgtttaggtatgcgtccatggatcgctgtagcttactcagctccagtagcagcagcttcagctgtattcttagtttaccctatcggccaaggttcattctctgacggtatgcctttaggtatctctggtactttcaacttcatgatcgtattccaagcagaacacaacatccttatgcacccattccacatgttaggtgttgctggtgtattcggtggttcattattctcagctatgcacggttctttagttacttcatctttaatccgtgaaacaactgaaaacgaatcagctaacgaaggttaccgtttcggtcaagaagaagaaacttacaacattgtagctgctcatggttactttggtcgtctaatcttccaatacgcttctttcaacaactctcgttcattacacttcttcttagctgcttggccggtaatcggtatttggttcactgctttaggtttatcaactatggcattcaacttaaacggtttcaacttcaaccaatcagtagtagactcacaaggtcgtgtactaaacacttgggcagacatcatcaaccgtgctaacttaggtatggaagtaatgcacgagcgtaacgctcacaacttcccactagacttagcttcaactaactctagctcaaacaactaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z