BBa_K200009 1 BBa_K200009 DpnII Composite 2009-08-11T11:00:00Z 2015-05-08T01:11:21Z Ecoli Dam Sensitive Restriction Enzyme. Recognition Sequence and Cutting site NGATCN -> N GATCN NCTAGN NCTAG N false false _298_ 0 5275 9 Not in stock false Dam Sensitivity false David Roche, Royah Vaezi component2059399 1 BBa_B0034 component2059400 1 BBa_K200015 annotation2059400 1 BBa_K200015 range2059400 1 19 888 annotation2059399 1 BBa_B0034 range2059399 1 1 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K200015 1 BBa_K200015 Coding region for the DpnII restriction enzyme. 2009-10-13T11:00:00Z 2015-05-08T01:11:21Z Synthesised from the genomic sequence. This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37??C. DpnII is a 4 base cutter, leaving a 4 base overhang. <br> The cutting site is as shown: <br><br> [[Image:II09_DpnII.png]] <br><br> false false _298_ 0 5155 9 Not in stock false NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0. false Royah Vaezi BBa_K200015_sequence 1 atgaaacagacccgtaacttcgacgagtggctgtctacaatgaccgataccgttgctgactggacctattatacagattttccgaaagtctataaaaacgtgagcagcatcaaagttgccctgaacatcatgaatagcctgattggcagtaaaaacatccaagaggattttctggacctgtatcagaactatccggagatcctgaaagtggttccactgctgatcgctaaacgtctgcgcgatacgattatcgtgaaagacccgattaaagatttctatttcgacttctccaaacgtaactatagcatcgaggagtataccatgttcctggaaaaatcgggcatcttcgacctgctgcaaaaccatctggtgtccaacctggttgactatgtgactggcgttgaggtgggtatggatacgaatggccgtaaaaaccgtactggtgacgctatggaaaacatcgttcagtcctatctggaagcagagggctatattctgggcgaaaacctgttcaaagagatcgaacaaaacgagattgaggaaattttcagcgtagacctgagcgccattaccaatgatggcaacacggttaaacgcttcgatttcgtcatcaaaaacgaacaagtgctgtatctgatcgaggtgaacttctattctggtagcggcagcaaactgaatgagactgcccgctcgtataaaatgatcgccgaggaaaccaaagcaattccgaacgtggagttcatgtggattacggacggtcaggggtggtataaagccaaaaacaacctgcgtgaaaccttcgacattctgccgtttctgtataacattaacgacctggagcataacattctgaaaaacctgaaatgatga BBa_B0034_sequence 1 aaagaggagaaa BBa_K200009_sequence 1 aaagaggagaaatactagatgaaacagacccgtaacttcgacgagtggctgtctacaatgaccgataccgttgctgactggacctattatacagattttccgaaagtctataaaaacgtgagcagcatcaaagttgccctgaacatcatgaatagcctgattggcagtaaaaacatccaagaggattttctggacctgtatcagaactatccggagatcctgaaagtggttccactgctgatcgctaaacgtctgcgcgatacgattatcgtgaaagacccgattaaagatttctatttcgacttctccaaacgtaactatagcatcgaggagtataccatgttcctggaaaaatcgggcatcttcgacctgctgcaaaaccatctggtgtccaacctggttgactatgtgactggcgttgaggtgggtatggatacgaatggccgtaaaaaccgtactggtgacgctatggaaaacatcgttcagtcctatctggaagcagagggctatattctgggcgaaaacctgttcaaagagatcgaacaaaacgagattgaggaaattttcagcgtagacctgagcgccattaccaatgatggcaacacggttaaacgcttcgatttcgtcatcaaaaacgaacaagtgctgtatctgatcgaggtgaacttctattctggtagcggcagcaaactgaatgagactgcccgctcgtataaaatgatcgccgaggaaaccaaagcaattccgaacgtggagttcatgtggattacggacggtcaggggtggtataaagccaaaaacaacctgcgtgaaaccttcgacattctgccgtttctgtataacattaacgacctggagcataacattctgaaaaacctgaaatgatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z