BBa_K200015 1 BBa_K200015 Coding region for the DpnII restriction enzyme. 2009-10-13T11:00:00Z 2015-05-08T01:11:21Z Synthesised from the genomic sequence. This is a Type II restriction enzyme that recognises the nucleotide sequence 'GATC.' Its activity is blocked by dam methylation. The working temperature is 37??C. DpnII is a 4 base cutter, leaving a 4 base overhang. <br> The cutting site is as shown: <br><br> [[Image:II09_DpnII.png]] <br><br> false false _298_ 0 5155 9 Not in stock false NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0. false Royah Vaezi BBa_K200015_sequence 1 atgaaacagacccgtaacttcgacgagtggctgtctacaatgaccgataccgttgctgactggacctattatacagattttccgaaagtctataaaaacgtgagcagcatcaaagttgccctgaacatcatgaatagcctgattggcagtaaaaacatccaagaggattttctggacctgtatcagaactatccggagatcctgaaagtggttccactgctgatcgctaaacgtctgcgcgatacgattatcgtgaaagacccgattaaagatttctatttcgacttctccaaacgtaactatagcatcgaggagtataccatgttcctggaaaaatcgggcatcttcgacctgctgcaaaaccatctggtgtccaacctggttgactatgtgactggcgttgaggtgggtatggatacgaatggccgtaaaaaccgtactggtgacgctatggaaaacatcgttcagtcctatctggaagcagagggctatattctgggcgaaaacctgttcaaagagatcgaacaaaacgagattgaggaaattttcagcgtagacctgagcgccattaccaatgatggcaacacggttaaacgcttcgatttcgtcatcaaaaacgaacaagtgctgtatctgatcgaggtgaacttctattctggtagcggcagcaaactgaatgagactgcccgctcgtataaaatgatcgccgaggaaaccaaagcaattccgaacgtggagttcatgtggattacggacggtcaggggtggtataaagccaaaaacaacctgcgtgaaaccttcgacattctgccgtttctgtataacattaacgacctggagcataacattctgaaaaacctgaaatgatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z