BBa_K200006 1 BBa_K200006 OtsB: Part 2 of 2 coding for trehalose producing enzymes 2009-08-11T11:00:00Z 2015-05-08T01:11:21Z Coding sequence isolated from e-coli Codes for trehalose-phosphatase enzyme. This second of two enzymes required for the conversion of glucose to trehalose. Trehalose is used to resist dessication in cells, and protect against conformational change in proteins under such conditions. false false _298_ 0 5275 9 It's complicated false Used to resist dessication in cells false Royah Vaezi, David Roche annotation2027320 1 Forward Primer range2027320 1 1 19 annotation2027322 1 stop range2027322 1 799 801 annotation2027318 1 OtsB range2027318 1 1 798 annotation2027319 1 start range2027319 1 1 3 annotation2027323 1 stop range2027323 1 802 804 annotation2027321 1 Reverse Primer range2027321 1 780 801 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K200017 1 BBa_K200017 RBS + OtsB 2009-10-14T11:00:00Z 2015-05-08T01:11:21Z PCR of OtsB from Escherichia Coli BL21(DE3, ligated to RBS extracted from Registry Composite part containing RBS for ligation to OtsA and promoter for production of trehalose. false false _298_ 0 5155 9 It's complicated false N/A false Royah Vaezi component2041322 1 BBa_K200006 component2041315 1 BBa_B0034 annotation2041315 1 BBa_B0034 range2041315 1 1 12 annotation2041322 1 BBa_K200006 range2041322 1 19 822 BBa_K200017_sequence 1 aaagaggagaaatactagatgacagaaccgttaaccgaaacccctgaactatccgcgaaatatgcctggttttttgatcttgatggaacgctggcggaaatcaaaccgcatcccgatcaggtcgtcgtgcctgacaatattctgcaaggactacagctactggcaaccgcaagtgatggtgcattggcattgatatcagggcgctcaatggtggagcttgacgcactggcaaaaccttatcgcttcccgttagcgggcgtgcatggggcggagcgccgtgacatcaatggtaaaacacatatcgttcatctgccggatgcgattgcgcgtgatattagcgtgcaactgcatacagtcatcgctcagtatcccggcgcggagctggaggcgaaagggatggcttttgcgctgcattatcgtcaggctccgcagcatgaagacgcattaatgacattagcgcaacgtattactcagatctggccacaaatggcgttacagcagggaaagtgtgttgtcgagatcaaaccgagaggtaccagtaaaggtgaggcaattgcagcttttatgcaggaagctccctttatcgggcgaacgcccgtatttctgggcgatgatttaaccgatgaatctggcttcgcagtcgttaaccgactgggcggaatgtcagtaaaaattggcacaggtgcaactcaggcatcatggcgactggcgggtgtgccggatgtctggagctggcttgaaatgataaccaccgcattacaacaaaaaagagaaaataacaggagtgatgactatgagtcgtttagtcgtagtatctaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K200006_sequence 1 atgacagaaccgttaaccgaaacccctgaactatccgcgaaatatgcctggttttttgatcttgatggaacgctggcggaaatcaaaccgcatcccgatcaggtcgtcgtgcctgacaatattctgcaaggactacagctactggcaaccgcaagtgatggtgcattggcattgatatcagggcgctcaatggtggagcttgacgcactggcaaaaccttatcgcttcccgttagcgggcgtgcatggggcggagcgccgtgacatcaatggtaaaacacatatcgttcatctgccggatgcgattgcgcgtgatattagcgtgcaactgcatacagtcatcgctcagtatcccggcgcggagctggaggcgaaagggatggcttttgcgctgcattatcgtcaggctccgcagcatgaagacgcattaatgacattagcgcaacgtattactcagatctggccacaaatggcgttacagcagggaaagtgtgttgtcgagatcaaaccgagaggtaccagtaaaggtgaggcaattgcagcttttatgcaggaagctccctttatcgggcgaacgcccgtatttctgggcgatgatttaaccgatgaatctggcttcgcagtcgttaaccgactgggcggaatgtcagtaaaaattggcacaggtgcaactcaggcatcatggcgactggcgggtgtgccggatgtctggagctggcttgaaatgataaccaccgcattacaacaaaaaagagaaaataacaggagtgatgactatgagtcgtttagtcgtagtatctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z