LOCUS       BBa_K200022             1812 bp    DNA     linear       08-Dec-2025
DEFINITION  Heat-inducible system with GFP reporter
ACCESSION   BBa_K200022
VERSION     BBa_K200022.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..769
                     /label=cIts
     CDS             56..769
                     /label=BBa_K098997
     misc_feature    44..769
                     /label=BBa_K098998
     stem_loop       785..804
                     /label=T7 TE
     misc_feature    811..811
                     /label=stop
     polyA_site      805..818
                     /label=polya
     misc_feature    778..818
                     /label=BBa_B0012
     terminator      778..818
                     /label=BBa_B0012
     stem_loop       839..861
                     /label=stem_loop
     misc_feature    827..872
                     /label=BBa_B0011
     terminator      827..872
                     /label=BBa_B0011
     promoter        918..923
                     /label=-10
     promoter        895..900
                     /label=-35
     misc_feature    905..921
                     /label=OR1
     misc_feature    881..897
                     /label=OR2
     misc_feature    881..929
                     /label=BBa_R0051
     promoter        881..929
                     /label=BBa_R0051
     misc_feature    778..929
                     /label=BBa_S04136
     misc_feature    44..929
                     /label=BBa_S04137
     misc_feature    1..929
                     /label=BBa_K098995
     misc_feature    942..945
                     /label=conserved
     RBS             938..949
                     /label=BBa_B0034
     CDS             956..1675
                     /label=GFP protein
     CDS             956..1675
                     /label=BBa_E0040
     stem_loop       1695..1738
                     /label=stem_loop
     misc_feature    1684..1763
                     /label=BBa_B0010
     terminator      1684..1763
                     /label=BBa_B0010
     stem_loop       1779..1798
                     /label=T7 TE
     misc_feature    1805..1805
                     /label=stop
     polyA_site      1799..1812
                     /label=polya
     misc_feature    1772..1812
                     /label=BBa_B0012
     terminator      1772..1812
                     /label=BBa_B0012
     misc_feature    938..1812
                     /label=BBa_I13504
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgag
       61 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
      121 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
      181 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
      241 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
      301 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
      361 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt
      421 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
      481 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
      541 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
      601 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
      661 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
      721 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat actagagtca
      781 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
      841 gccagattat taatccggct tttttattat tttactagag taacaccgtg cgtgttgact
      901 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatgcg
      961 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1021 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1081 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1141 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1201 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1261 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1321 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1381 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1441 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1501 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1561 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1621 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     1681 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1741 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1801 tctgcgttta ta
//