LOCUS       BBa_K200026             1537 bp    DNA     linear       22-Dec-2024
DEFINITION  RcsB+RBS+GFP+TT
ACCESSION   BBa_K200026
VERSION     BBa_K200026.1  
FEATURES             Location/Qualifiers
     CDS             1..648
                     /label=RcsB gene
     misc_feature    1..3
                     /label=start
     misc_feature    649..653
                     /label=stop
     CDS             1..654
                     /label=BBa_K200000
     misc_feature    667..670
                     /label=conserved
     RBS             663..674
                     /label=BBa_B0034
     CDS             681..1400
                     /label=GFP protein
     CDS             681..1400
                     /label=BBa_E0040
     stem_loop       1420..1463
                     /label=stem_loop
     misc_feature    1409..1488
                     /label=BBa_B0010
     terminator      1409..1488
                     /label=BBa_B0010
     stem_loop       1504..1523
                     /label=T7 TE
     misc_feature    1530..1530
                     /label=stop
     polyA_site      1524..1537
                     /label=polya
     misc_feature    1497..1537
                     /label=BBa_B0012
     terminator      1497..1537
                     /label=BBa_B0012
ORIGIN
        1 atgaacaata tgaacgtaat tattgccgat gaccatccga tagtcttgtt cggtattcgc
       61 aaatcacttg agcaaattga gtgggtgaat gttgtcggcg aatttgaaga ctctacagca
      121 ctgatcaaca acctgccgaa actggatgcg catgtgttga ttaccgatct ctccatgcct
      181 ggcgataagt acggcgatgg cattacctta atcaagtaca tcaagcgcca tttcccaagc
      241 ctgtcgatca ttgttctgac tatgaacaac aacccggcga ttcttagtgc ggtattggat
      301 ctggatatcg aagggatcgt gctgaaacaa ggtgcaccga ccgatctgcc gaaagctctc
      361 gccgcgctcc agaaagggaa gaaatttacc ccggaaagcg tttctcgcct gttggaaaaa
      421 atcagtgctg gtggttacgg tgacaagcgt ctctcgccaa aagagagtga agttctgcgc
      481 ctgtttgcgg aaggcttcct ggtgaccgag atcgctaaaa agctgaaccg cagtattaaa
      541 accatcagta gccagaagaa atctgcgatg atgaagctgg gtgtcgagaa cgatatcgcc
      601 ctgctgaatt atctctcttc agtgacctta agtccggcag ataaagacta ataatactag
      661 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      721 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      781 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      841 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      901 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      961 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
     1021 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
     1081 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
     1141 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
     1201 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     1261 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     1321 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     1381 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1441 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1501 actggctcac cttcgggtgg gcctttctgc gtttata
//