LOCUS       BBa_K200027             2245 bp    DNA     linear       22-Nov-2024
DEFINITION  PAH+RBS+GFP+TT
ACCESSION   BBa_K200027
VERSION     BBa_K200027.1  
FEATURES             Location/Qualifiers
     CDS             1..1362
                     /label=PAH
     misc_feature    1360..1362
                     /label=PAH
     misc_feature    1..3
                     /label=PAH
     primer_bind     1..16
                     /label=Forward primer
     primer_bind     1341..1362
                     /label=Reverse primer
     CDS             1..1362
                     /label=BBa_K200008
     misc_feature    1375..1378
                     /label=conserved
     RBS             1371..1382
                     /label=BBa_B0034
     CDS             1389..2108
                     /label=GFP protein
     CDS             1389..2108
                     /label=BBa_E0040
     stem_loop       2128..2171
                     /label=stem_loop
     misc_feature    2117..2196
                     /label=BBa_B0010
     terminator      2117..2196
                     /label=BBa_B0010
     stem_loop       2212..2231
                     /label=T7 TE
     misc_feature    2238..2238
                     /label=stop
     polyA_site      2232..2245
                     /label=polya
     misc_feature    2205..2245
                     /label=BBa_B0012
     terminator      2205..2245
                     /label=BBa_B0012
ORIGIN
        1 atgtccactg cggtcctgga aaacccaggc ttgggcagga aactctctga ctttggacag
       61 gaaacaagct atattgaaga caactgcaat caaaatggtg ccatatcgct gatcttctca
      121 ctcaaagaag aagttggtgc attggccaaa gtattgcgct tatttgagga gaatgatgta
      181 aacctgaccc acattgaatc cagaccttct cgtttaaaga aagatgagta tgaatttttc
      241 acccatttgg ataaacgtag cctgcctgct ctgacaaaca tcatcaagat cttgaggcat
      301 gacattggtg ccactgtcca tgagctttca cgagataaga agaaagacac agtgccctgg
      361 ttcccaagaa ccattcaaga gctggacaga tttgccaatc agattctcag ctatggagcg
      421 gaactggatg ctgaccaccc tggttttaaa gatcctgtgt accgtgcaag acggaagcag
      481 tttgctgaca ttgcctacaa ctaccgccat gggcagccca tccctcgagt ggaatacatg
      541 gaggaaggaa agaaaacatg gggcacagtg ttcaagactc tgaagtcctt gtataaaacc
      601 catgcttgct atgagtacaa tcacattttt ccacttcttg aaaagtactg tggcttccat
      661 gaagataaca ttccccagct ggaagacgtt tctcaattcc ttcagacttg cactggtttc
      721 cgcctccgac ctgtggctgg cctgctttcc tctcgggatt tcttgggtgg cctggccttc
      781 cgagtcttcc actgcacaca gtacatcaga catggatcca agcccatgta tacccccgaa
      841 cctgacatct gccatgagct gttgggacat gtgcccttgt tttcagatcg cagctttgcc
      901 cagttttccc aggaaattgg ccttgcctct ctgggtgcac ctgatgaata cattgaaaag
      961 ctcgccacaa tttactggtt tactgtggag tttgggctct gcaaacaagg agactccata
     1021 aaggcatatg gtgctgggct cctgtcatcc tttggtgaat tacagtactg cttatcagag
     1081 aagccaaagc ttctccccct ggagctggag aagacagcca tccaaaatta cactgtcacg
     1141 gagttccagc ccctctatta cgtggcagag agttttaatg atgccaagga gaaagtaagg
     1201 aactttgctg ccacaatacc tcggcccttc tcagttcgct acgacccata cacccaaagg
     1261 attgaggtct tggacaatac ccagcagctt aagattttgg ctgattccat taacagtgaa
     1321 attggaatcc tttgcagtgc cctccagaaa ataaagtaat aatactagag aaagaggaga
     1381 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1441 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1501 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1561 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1621 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1681 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1741 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1801 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1861 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1921 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1981 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     2041 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     2101 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2161 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2221 tcgggtgggc ctttctgcgt ttata
//