LOCUS       BBa_K200031             1719 bp    DNA     linear       01-Jun-2024
DEFINITION  IPTG inducible LipA
ACCESSION   BBa_K200031
VERSION     BBa_K200031.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1582
                     /label=BBa_K200030
     stem_loop       1602..1645
                     /label=stem_loop
     misc_feature    1591..1670
                     /label=BBa_B0010
     terminator      1591..1670
                     /label=BBa_B0010
     stem_loop       1686..1705
                     /label=T7 TE
     misc_feature    1712..1712
                     /label=stop
     polyA_site      1706..1719
                     /label=polya
     misc_feature    1679..1719
                     /label=BBa_B0012
     terminator      1679..1719
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgcaaatg acggaagcta cctgttgacg ctgctgtaaa
      121 accggagcaa aggggattgt atttgccggt tctgggaacg ggtctttatc tgatgcagcc
      181 gaaaaagggg cggacagcgc agtcaaaaaa ggcgttacag tggtgcgctc tacccgcacg
      241 ggaaatggtg tcgtcacacc aaaccaagac tatgcggaaa aggacttgct ggcatcgaac
      301 tctttaaacc cccaaaaagc acggatgttg ctgatcgttg ctcttaccaa aacaaatgat
      361 cctcaaaaaa tccaagctta tttcaatgag tattgaagaa aagaaggcga ataagccttc
      421 ttttttttgg ctttttagga ccaataatga cctctgaatc ttaaaatttc tttaaaaata
      481 agccaaaatt accctttact taattaattt ggtaacgtaa tataattgga gaatttgtta
      541 caaaaaaagg aggatattat gaaatttgta aaaagaagga tcattgcact tgtaacaatt
      601 ttgatgctgt ctgttacatc gctgtttgcg ttgcagccgt cagcaaaagc cgctgaacac
      661 aatccagtcg ttatggttca cggtattgga ggggcatcat tcaattttgc gggaattaag
      721 agctatctcg tatctcaggg ctggtcgcgg gacaagctgt atgcagttga tttttgggac
      781 aagacaggca caaattataa caatggaccg gtattatcac gatttgtgca aaaggtttta
      841 gatgaaacgg gtgcgaaaaa agtggatatt gtcgctcaca gcatgggggg cgcgaacaca
      901 ctttactaca taaaaaatct ggacggcgga aataaagttg caaacgtcgt gacggttggc
      961 ggcgcgaacc gtttgacgac aggcaaggcg cttccgggaa cagatccaaa tcaaaagatt
     1021 ttatacacat ccatttacag cagtgccgat atgattgtca tgaattactt atcaagatta
     1081 gatggtgcta gaaacgttca aatccatggc gttggacaca tcggccttct gtacagcagc
     1141 caagtcaaca gcctgattaa agaagggctg aacggcgggg gccagaatac gaattaatga
     1201 aaaacaaaac cttgaagaat gctattcttc aaggttattc tgctttcagc acaatggttt
     1261 tcgcagccat atcatgaacg gtttgttttt tcttcgtaaa tgcggcagtc aaatagatca
     1321 ggcgggagaa cacatgcacc cacgctatca ggtaacggac aatggcttgc gggaaggata
     1381 tttttttata tgtttcgtcc ctcacgattt gcagcccgat gctttttttg cccagtgtgc
     1441 ccttccaatt tgtcagcggc atcagcaaaa aatacacaat cagcatcaat atggcgacaa
     1501 taatgacacc ggcggaccca tcgccaaacg taaatccggc tgccaaaatc actgctgcgc
     1561 aatgattaca tcaagttaat aatactagag ccaggcatca aataaaacga aaggctcagt
     1621 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1681 acactggctc accttcgggt gggcctttct gcgtttata
//