LOCUS BBa_K2003002 5286 bp DNA linear 10-Jun-2024 DEFINITION pBad+RBS+CPF1+Terminator ACCESSION BBa_K2003002 VERSION BBa_K2003002.1 FEATURES Location/Qualifiers promoter 1..1190 /label=BBa_I0500 Pbad/araC RBS 1199..1211 /label=BBa_0032 Medium RBS CDS 1226..5149 /label=His-tagged CPF1 misc_feature 5158..5286 /label=BBa_0015 Double terminator ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat tactagagtc 1201 acacaggaaa gtactagaga tggcacatca tcatcatcat catatgtcca tttaccaaga 1261 gtttgttaac aagtacagcc tgagtaaaac tcttcgtttc gaactaatcc ctcagggtaa 1321 gactttggaa aatataaagg ctaggggtct gatcttagat gacgagaagc gtgctaagga 1381 ttataaaaag gccaaacaga ttattgataa atatcaccag ttctttatag aggaaatcct 1441 tagttccgta tgtatttcag aggatctact tcagaattac tctgatgttt acttcaaact 1501 aaagaaatca gacgacgata acctacaaaa agattttaaa agcgcaaagg atacaataaa 1561 gaaacagata tccgaataca tcaaagattc agagaaattt aaaaatctat ttaaccaaaa 1621 tctaattgat gcaaagaaag gacaggagtc agacttaatt ttgtggctta agcagagtaa 1681 agataatggt atcgaactgt ttaaggcgaa cagtgacatt acagatattg acgaagcgtt 1741 ggaaatcatc aaatccttta agggttggac aacgtatttc aagggctttc atgaaaatcg 1801 taaaaacgtt tacagtagca atgacatccc tacgtcaatc atctacagga tagttgatga 1861 caacttgccc aaattcttgg agaataaggc aaagtacgaa tcactgaagg acaaagcacc 1921 cgaagcgata aactatgagc agattaaaaa agacttggcg gaagagttga cgttcgacat 1981 agactacaaa acatccgaag ttaaccagag agtatttagt ctggacgaag tattcgagat 2041 tgccaacttt aacaactacc ttaaccaaag cgggattaca aagttcaaca ccatcattgg 2101 tggaaagttt gtgaatggag agaacaccaa gaggaaaggc ataaacgaat atataaacct 2161 gtactcccaa cagataaatg acaagacatt gaaaaagtac aagatgtccg tcctatttaa 2221 acaaatcttg agtgacaccg aaagcaaatc atttgtcatt gataaactgg aggacgatag 2281 cgatgtggtc accacaatgc agagtttcta tgagcagatt gccgcgttca aaacagtcga 2341 ggagaaatcc attaaggaaa ccttgagtct gcttttcgat gatctgaaag cacaaaaact 2401 ggacctttca aagatatatt ttaaaaacga taagtcatta accgacttat cccaacaggt 2461 ctttgacgat tactccgtta tcgggacggc cgtcctggaa tatatcacac agcagatagc 2521 gccgaaaaac ttagataatc cttcaaaaaa agagcaggag ctgatcgcaa agaagacaga 2581 aaaagcaaaa tacctgagtt tagagacgat caagctggcg ctggaagagt ttaacaaaca 2641 tcgtgacatt gataaacagt gtagattcga agagatctta gctaacttcg cggccatacc 2701 catgatattt gacgagatcg ctcagaataa agataactta gcacagatat ctatcaagta 2761 tcagaatcaa ggaaaaaagg accttttaca ggcttcagca gaggatgatg ttaaggcgat 2821 taaggatcta ctagatcaga caaataatct gctacacaag ttgaagatct ttcatataag 2881 ccaatcagag gacaaagcga atatattaga caaagatgaa catttctatc ttgtgtttga 2941 agaatgctat tttgagttag caaatatcgt tccattgtac aacaaaatta ggaattatat 3001 tacacagaag ccgtattctg acgagaaatt taaattgaac tttgaaaaca gcactctggc 3061 gaatggttgg gataaaaata aagaacctga caacaccgca atcttgttca tcaaagacga 3121 taagtactat ctaggtgtga tgaacaagaa aaataacaaa atcttcgatg ataaggcaat 3181 taaggaaaat aagggcgaag gctataaaaa gatcgtatat aagttactac caggtgcgaa 3241 caaaatgctt ccaaaagtct tcttctctgc gaaatctatc aagttctata accctagcga 3301 ggatattcta cgtatacgta atcactccac tcacactaag aacgggagcc cccaaaaagg 3361 atacgagaaa tttgagttca acattgagga ttgccgtaaa tttatagatt tttacaagca 3421 aagcatttca aagcacccag aatggaaaga ttttgggttc aggttttcag acactcaaag 3481 gtataatagc atagatgaat tttacaggga ggtagagaat cagggataca agttgacatt 3541 cgagaatatt agtgagtctt acatagattc tgtcgttaac caaggaaagt tgtacctatt 3601 ccaaatttac aacaaagact ttagcgcata tagtaagggg aggcccaacc tgcatacgtt 3661 atattggaag gcattatttg acgaacgtaa tctacaggac gtcgtctaca agctaaatgg 3721 ggaggctgaa cttttctata ggaaacaatc cattccgaaa aaaatcaccc atcctgcaaa 3781 agaggctatt gcgaacaaaa ataaagataa tccaaaaaaa gagagtgttt ttgaatacga 3841 cttgataaag gataaaaggt tcaccgagga caaatttttt tttcattgtc cgattacgat 3901 aaattttaaa tcatccggcg cgaataaatt caacgatgag ataaatctac tactgaagga 3961 aaaagccaac gatgttcaca ttctgagtat tgatcgtggt gaaaggcacc tggcctacta 4021 cacgttagtt gatggtaagg gcaacattat aaagcaggac acgttcaaca taattggtaa 4081 cgatcgtatg aaaaccaact atcatgataa actagcggct atcgaaaaag atagggattc 4141 cgctcgtaag gattggaaaa aaataaataa cataaaggaa atgaaagagg gctacttatc 4201 ccaagttgtc catgaaatcg cgaaattagt aattgagtac aacgcaatcg ttgtattcga 4261 agacctgaat ttcgggttca agcgtggaag gttcaaggtt gagaaacaag tctaccagaa 4321 attagaaaag atgttaatag aaaagctgaa ctacttggtg ttcaaggata atgagtttga 4381 taaaactggc ggggttcttc gtgcttatca attaacagcc ccctttgaga cgttcaaaaa 4441 aatgggcaaa cagaccggca tcatttacta cgtgcccgca ggattcactt caaagatatg 4501 ccccgtgaca ggatttgtca accaacttta tcctaaatat gagagcgtga gcaagtccca 4561 agagttcttt agtaaattcg acaaaatctg ttataacttg gacaagggct atttcgaatt 4621 tagctttgac tacaaaaact ttggcgacaa agctgcaaag ggtaagtgga ccatcgcgag 4681 ttttggctcc aggctgataa atttcagaaa ttccgataag aatcataact gggatacccg 4741 tgaagtctat cccacgaagg aattggagaa gctgctaaag gactactcca tcgaatacgg 4801 ccatggagag tgtataaagg cggcaatatg tggtgaatct gataaaaaat ttttcgccaa 4861 gttgacatct gttttaaaca ccatcctaca aatgcgtaat agcaagactg ggactgaact 4921 ggactatcta atcagtcccg tcgccgatgt aaatgggaat ttcttcgact caaggcaagc 4981 ccctaaaaac atgccacaag acgcagatgc taatggtgcc taccatattg ggttaaaggg 5041 gctaatgtta ctggggcgta ttaaaaataa ccaggaaggt aagaagctga atctggtaat 5101 taagaatgaa gaatattttg aatttgtgca gaaccgtaac aattaataat actagagcca 5161 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 5221 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 5281 tttata //