LOCUS       BBa_K2004002             846 bp    DNA     linear       25-Nov-2024
DEFINITION  CDO+polyHis tag
ACCESSION   BBa_K2004002
VERSION     BBa_K2004002.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..55
                     /label=BBa_J61101
     CDS             62..665
                     /label=BBa_K2004007
     misc_feature    674..703
                     /label=10x-Histidine Tag
     misc_feature    704..706
                     /label=Stop
     misc_feature    707..709
                     /label=Stop
     misc_feature    674..709
                     /label=BBa_K844000
     stem_loop       729..772
                     /label=stem_loop
     misc_feature    718..797
                     /label=BBa_B0010
     terminator      718..797
                     /label=BBa_B0010
     stem_loop       813..832
                     /label=T7 TE
     misc_feature    839..839
                     /label=stop
     polyA_site      833..846
                     /label=polya
     misc_feature    806..846
                     /label=BBa_B0012
     terminator      806..846
                     /label=BBa_B0012
     terminator      718..846
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagaca ggacctacta
       61 gatgaccgcg ccgcgcgtgg aaaaactgcg ccagtttatt caggatctgg atgcgctgca
      121 tcgccagttt accgatgaag cgaccctgct ggatgcggtg gcggtgcgcc tggcgagcct
      181 ggtgcagaaa gatgattggc tgccggaaga atataccctg ccgcatccgc atcattatca
      241 gcagtatctg ctgcatgcgg atagcggcca gcgctttagc attgtgagct ttgtgtgggg
      301 cccgggccag agcaccccga ttcatgatca tcgcgtgtgg ggcgcgattg gcatgctgcg
      361 cggcgcggaa gaaaaccagc gctatcgcct ggatgaacgc ggcctgccga ttgcgaccgg
      421 cccggcggaa cgcctgagcc cgggcaaagt ggaaaaagtg agcagccgcg atggcgatat
      481 tcatcgcgtg agcaacgcgc tggcggatca tgtgagcatt agcattcatg tgtatggcgc
      541 gaacattggc ggcgtgcgcc gcgcggtgta taccgcggat ggcattgcga aaccgtttgt
      601 gagcggctat agcaaccgcc atctgccgaa catttgggat ctgagcaaag ataacgaata
      661 tgcgatacta gagcatcatc accatcacca ccatcatcac cattaataat actagagcca
      721 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      781 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      841 tttata
//