BBa_K2004003
1
BBa_K2004003
CDO HIS
2016-10-17T11:00:00Z
2016-10-18T12:29:00Z
NCBI data bank. Enterobacter microorganism.
This device is a construct for constitutive expression of CDO enzyme with a poly-his tag attached downstream to ease the protein purification, this device could be used to ease the assemble with other parts of the registry.
For more information about this enzyme, look for BBa_K2004007.
false
false
_2471_
25099
25099
9
Not in stock
false
Codon optimized for E. coli and cleaned up from restriction sites.
false
Marco V. Lincango Y.
component2517446
1
BBa_J61101
component2517445
1
BBa_J23101
component2517451
1
BBa_K844000
component2517447
1
BBa_K2004007
annotation2517451
1
BBa_K844000
range2517451
1
674
709
annotation2517447
1
BBa_K2004007
range2517447
1
62
665
annotation2517445
1
BBa_J23101
range2517445
1
1
35
annotation2517446
1
BBa_J61101
range2517446
1
44
55
BBa_K2004007
1
CDO
Cysteine dioxygenase (CDO)
2016-10-17T11:00:00Z
2016-10-18T11:17:59Z
The sequence of this part was taken from the NCBI databank, and the original sequence belongs to Enterobacter aerogenes.
Cysteine dioxygenase (CDO, 2 EC 1.13.11.20) is a non-heme mononuclear iron metalloenzyme that catalyzes the irreversible oxidation of cysteine to cysteine sulfinic acid (CSA) with addition of molecular oxygen. This enzyme has a length of 200 aminoacids and a mass of 22,972 Daltons (UniProt, 2005).
The product of the reaction catalyzed by CDO, cysteine sulfinic acid, is either decarboxylated to hypotaurine, which is further oxidized to taurine by a poorly understood mechanism.
false
false
_2471_
25099
25099
9
false
This sequence was optimized to be able to use in E. coli in addition to be free of restriction sites into the coding region. This biobrick can be used in RFC 10.
false
Marco V. Lincango Y.
BBa_J61101
1
BBa_J61101
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
fix
false
false
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K844000
1
BBa_K844000
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
Constructed through oligonucleotide annealing
Released HQ 2013
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
false
false
_1104_
0
9404
9
In stock
true
none
false
Kathleen Miller
annotation2206607
1
10x-Histidine Tag
range2206607
1
1
30
annotation2206609
1
Stop
range2206609
1
34
36
annotation2206608
1
Stop
range2206608
1
31
33
BBa_J61101_sequence
1
aaagacaggacc
BBa_K2004003_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagacaggacctactagatgaccgcgccgcgcgtggaaaaactgcgccagtttattcaggatctggatgcgctgcatcgccagtttaccgatgaagcgaccctgctggatgcggtggcggtgcgcctggcgagcctggtgcagaaagatgattggctgccggaagaatataccctgccgcatccgcatcattatcagcagtatctgctgcatgcggatagcggccagcgctttagcattgtgagctttgtgtggggcccgggccagagcaccccgattcatgatcatcgcgtgtggggcgcgattggcatgctgcgcggcgcggaagaaaaccagcgctatcgcctggatgaacgcggcctgccgattgcgaccggcccggcggaacgcctgagcccgggcaaagtggaaaaagtgagcagccgcgatggcgatattcatcgcgtgagcaacgcgctggcggatcatgtgagcattagcattcatgtgtatggcgcgaacattggcggcgtgcgccgcgcggtgtataccgcggatggcattgcgaaaccgtttgtgagcggctatagcaaccgccatctgccgaacatttgggatctgagcaaagataacgaatatgcgatactagagcatcatcaccatcaccaccatcatcaccattaataa
BBa_K844000_sequence
1
catcatcaccatcaccaccatcatcaccattaataa
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_K2004007_sequence
1
atgaccgcgccgcgcgtggaaaaactgcgccagtttattcaggatctggatgcgctgcatcgccagtttaccgatgaagcgaccctgctggatgcggtggcggtgcgcctggcgagcctggtgcagaaagatgattggctgccggaagaatataccctgccgcatccgcatcattatcagcagtatctgctgcatgcggatagcggccagcgctttagcattgtgagctttgtgtggggcccgggccagagcaccccgattcatgatcatcgcgtgtggggcgcgattggcatgctgcgcggcgcggaagaaaaccagcgctatcgcctggatgaacgcggcctgccgattgcgaccggcccggcggaacgcctgagcccgggcaaagtggaaaaagtgagcagccgcgatggcgatattcatcgcgtgagcaacgcgctggcggatcatgtgagcattagcattcatgtgtatggcgcgaacattggcggcgtgcgccgcgcggtgtataccgcggatggcattgcgaaaccgtttgtgagcggctatagcaaccgccatctgccgaacatttgggatctgagcaaagataacgaatatgcga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z