BBa_K2004009 1 BBa_K2004009 CDO CSAD 2016-10-17T11:00:00Z 2016-10-18T12:54:42Z NCBI databank. Sequences taken from E. aerogenes and H. sapiens This device allows the entire enzymatic pathway works, taking the substrate, L-cysteine which will be used by CDO (Cysteine dioxygenase) to produce 3-Sulfino-L-alanine to finally convert this substrate in Hypotaurine through enzymatic reaction with CSAD(Cysteinesulfinic acid decarboxylase). false false _2471_ 25099 25099 9 false Codon optimized for E. coli and sequences free of restrictions sites. false Marco V. Lincango Y. component2517468 1 BBa_K2004007 component2517469 1 BBa_J61101 component2517470 1 BBa_K2004008 component2517477 1 BBa_B0015 component2517466 1 BBa_J23101 component2517467 1 BBa_J61101 annotation2517468 1 BBa_K2004007 range2517468 1 62 665 annotation2517467 1 BBa_J61101 range2517467 1 44 55 annotation2517466 1 BBa_J23101 range2517466 1 1 35 annotation2517477 1 BBa_B0015 range2517477 1 2264 2392 annotation2517470 1 BBa_K2004008 range2517470 1 692 2255 annotation2517469 1 BBa_J61101 range2517469 1 674 685 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K2004007 1 CDO Cysteine dioxygenase (CDO) 2016-10-17T11:00:00Z 2016-10-18T11:17:59Z The sequence of this part was taken from the NCBI databank, and the original sequence belongs to Enterobacter aerogenes. Cysteine dioxygenase (CDO, 2 EC 1.13.11.20) is a non-heme mononuclear iron metalloenzyme that catalyzes the irreversible oxidation of cysteine to cysteine sulfinic acid (CSA) with addition of molecular oxygen. This enzyme has a length of 200 aminoacids and a mass of 22,972 Daltons (UniProt, 2005). The product of the reaction catalyzed by CDO, cysteine sulfinic acid, is either decarboxylated to hypotaurine, which is further oxidized to taurine by a poorly understood mechanism. false false _2471_ 25099 25099 9 false This sequence was optimized to be able to use in E. coli in addition to be free of restriction sites into the coding region. This biobrick can be used in RFC 10. false Marco V. Lincango Y. BBa_J61101 1 BBa_J61101 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A fix false false _95_ 0 483 95 In stock false N/A true John Anderson BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K2004008 1 CSAD GADL1 Acidic Amino Acid Decarboxylase 2016-10-17T11:00:00Z 2016-10-18T11:34:57Z The sequence of this part was taken from NCBI databank, from the Homo sapiens genome sequence. GADL1 (Glutamate Decarboxylase Like 1) is a Protein Coding gene. Diseases associated with GADL1 include Gadl1-Related Altered Drug Metabolism. Among its related pathways are Pantothenate and CoA biosynthesis and Taurine and hypotaurine metabolism. GADL1 encodes a member of the group 2 decarboxylase family. A similar protein in rodents plays a role in multiple biological processes as the rate-limiting enzyme in taurine biosynthesis, catalyzing the decarboxylation of cysteine sulfinate to hypotaurine. The cysteine sulfinic acid decarboxylase enzyme (CSAD), also known as sulfoalanine decarboxylase, is an enzyme involved in the taurine biosynthesis. It is in charge of catalyzing the reaction that decarboxylates cysteine sulfinate generating hypotaurine and CO2. CSAD is a liase, and more specifically a carboxylase (Sumizu, K., 1962) false false _2471_ 25099 25099 9 false The sequence was cleaned up from restriction sites, to be able to use in a biobrick format. Furthermore, this part was codon optimized to E. coli. false Marco V. Lincango Y. BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J61101_sequence 1 aaagacaggacc BBa_K2004009_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagacaggacctactagatgaccgcgccgcgcgtggaaaaactgcgccagtttattcaggatctggatgcgctgcatcgccagtttaccgatgaagcgaccctgctggatgcggtggcggtgcgcctggcgagcctggtgcagaaagatgattggctgccggaagaatataccctgccgcatccgcatcattatcagcagtatctgctgcatgcggatagcggccagcgctttagcattgtgagctttgtgtggggcccgggccagagcaccccgattcatgatcatcgcgtgtggggcgcgattggcatgctgcgcggcgcggaagaaaaccagcgctatcgcctggatgaacgcggcctgccgattgcgaccggcccggcggaacgcctgagcccgggcaaagtggaaaaagtgagcagccgcgatggcgatattcatcgcgtgagcaacgcgctggcggatcatgtgagcattagcattcatgtgtatggcgcgaacattggcggcgtgcgccgcgcggtgtataccgcggatggcattgcgaaaccgtttgtgagcggctatagcaaccgccatctgccgaacatttgggatctgagcaaagataacgaatatgcgatactagagaaagacaggacctactagatgagcagcgacagtgatcgtcagtgtcctgtggatggcgatatagaccagcaggaaatgataccaagcaaaaaaaacgcggtgctggtggatggcgtggtgctgaacgggccgaccaccgatgcgaaagcgggcgaaaaattcgtcgaggaagcgtgccgcctgattatggaggaggtcgtcctcaaagcgacagatgtgaacgaaaaagtgtgcgaatggcgaccacctgaacagctaaaacagctacttgatctggagatgcgcgatagcggcgaaccgccacataaactgctggaactttgccgcgatgtgattcattatagcgtgaaaaccaaccatccgcgattttttaaccagctttatgcgggcctggattattatagcctggtggcgcgctttatgacagaagctcttaaccccagcgtttacacctatgaagtaagcccggtgtttctcctggtggaagaagcggtgctaaagaagatgatagagttcattggctggaaagaaggcgatggcatttttaatccaggcggaagcgtgagcaatatgtatgcgatgaacctggcgcgttataagtattgcccggatattaaagaaaaagggctttcgggcagcccgcgtctcatactgtttaccagcgcggaatgccactatagcatgaagaaagcggcgagctttctgggcattggcacagaaaatgtatgctttgtggaaaccgatggacggggcaaaatgataccagaagaattagaaaaacaggtgtggcaggcgcgcaaggaaggggcggccccgtttctggtgtgcgcgaccagcggcacaaccgttctgggcgcgtttgacccgctggatgaaattgcggatatttgcgaacgccatagcctgtggcttcatgttgatgcgagctggggtggcagcgccctgatgagccgcaaacatcgcaaactgctacatggcattcatcgcgcggatagcgtggcgtggaacccgcataaaatgctcatggcaggcattcagtgctgcgctctgctggtgaaagataaaagcgatctactgaaaaaatgttatagcgctaaggcgagctatctgtttcagcaggataaattttatgatgtgagctatgatacgggcgataagagcattcagtgtagccgccgcccggatgcgtttaagttttggatgacctggaaagctctgggaacgctgggcctggaagaacgagtgaaccgcgcgcttgcgctatctcgctatctggtggatgaaattaaaaaacgcgaaggctttaagctgctgatggaacctgaatatgcgaacatttgcttttggtatattcccccaagcctgcgcgaaatggaagaaggcccggaattttgggcgaaactgaatctggtggcccctgcgataaaagaacgcatgatgaaaaaaggcagcctgatgctgggctatcagccgcatcgcggcaaagtgaacttttttcgccaggtggtgattagcccacaggtgagccgcgaagatatggatttcctgctcgacgagattgatctactggggaaggatatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2004008_sequence 1 atgagcagcgacagtgatcgtcagtgtcctgtggatggcgatatagaccagcaggaaatgataccaagcaaaaaaaacgcggtgctggtggatggcgtggtgctgaacgggccgaccaccgatgcgaaagcgggcgaaaaattcgtcgaggaagcgtgccgcctgattatggaggaggtcgtcctcaaagcgacagatgtgaacgaaaaagtgtgcgaatggcgaccacctgaacagctaaaacagctacttgatctggagatgcgcgatagcggcgaaccgccacataaactgctggaactttgccgcgatgtgattcattatagcgtgaaaaccaaccatccgcgattttttaaccagctttatgcgggcctggattattatagcctggtggcgcgctttatgacagaagctcttaaccccagcgtttacacctatgaagtaagcccggtgtttctcctggtggaagaagcggtgctaaagaagatgatagagttcattggctggaaagaaggcgatggcatttttaatccaggcggaagcgtgagcaatatgtatgcgatgaacctggcgcgttataagtattgcccggatattaaagaaaaagggctttcgggcagcccgcgtctcatactgtttaccagcgcggaatgccactatagcatgaagaaagcggcgagctttctgggcattggcacagaaaatgtatgctttgtggaaaccgatggacggggcaaaatgataccagaagaattagaaaaacaggtgtggcaggcgcgcaaggaaggggcggccccgtttctggtgtgcgcgaccagcggcacaaccgttctgggcgcgtttgacccgctggatgaaattgcggatatttgcgaacgccatagcctgtggcttcatgttgatgcgagctggggtggcagcgccctgatgagccgcaaacatcgcaaactgctacatggcattcatcgcgcggatagcgtggcgtggaacccgcataaaatgctcatggcaggcattcagtgctgcgctctgctggtgaaagataaaagcgatctactgaaaaaatgttatagcgctaaggcgagctatctgtttcagcaggataaattttatgatgtgagctatgatacgggcgataagagcattcagtgtagccgccgcccggatgcgtttaagttttggatgacctggaaagctctgggaacgctgggcctggaagaacgagtgaaccgcgcgcttgcgctatctcgctatctggtggatgaaattaaaaaacgcgaaggctttaagctgctgatggaacctgaatatgcgaacatttgcttttggtatattcccccaagcctgcgcgaaatggaagaaggcccggaattttgggcgaaactgaatctggtggcccctgcgataaaagaacgcatgatgaaaaaaggcagcctgatgctgggctatcagccgcatcgcggcaaagtgaacttttttcgccaggtggtgattagcccacaggtgagccgcgaagatatggatttcctgctcgacgagattgatctactggggaaggatatga BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2004007_sequence 1 atgaccgcgccgcgcgtggaaaaactgcgccagtttattcaggatctggatgcgctgcatcgccagtttaccgatgaagcgaccctgctggatgcggtggcggtgcgcctggcgagcctggtgcagaaagatgattggctgccggaagaatataccctgccgcatccgcatcattatcagcagtatctgctgcatgcggatagcggccagcgctttagcattgtgagctttgtgtggggcccgggccagagcaccccgattcatgatcatcgcgtgtggggcgcgattggcatgctgcgcggcgcggaagaaaaccagcgctatcgcctggatgaacgcggcctgccgattgcgaccggcccggcggaacgcctgagcccgggcaaagtggaaaaagtgagcagccgcgatggcgatattcatcgcgtgagcaacgcgctggcggatcatgtgagcattagcattcatgtgtatggcgcgaacattggcggcgtgcgccgcgcggtgtataccgcggatggcattgcgaaaccgtttgtgagcggctatagcaaccgccatctgccgaacatttgggatctgagcaaagataacgaatatgcga BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z