LOCUS       BBa_K2004012            2460 bp    DNA     linear       15-Nov-2024
DEFINITION  CSAD and reporter
ACCESSION   BBa_K2004012
VERSION     BBa_K2004012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..55
                     /label=BBa_J61101
     CDS             62..1625
                     /label=BBa_K2004008
     RBS             1634..1645
                     /label=BBa_J61101
     misc_feature    1652..2323
                     /label=BBa_K2004004
     stem_loop       2343..2386
                     /label=stem_loop
     misc_feature    2332..2411
                     /label=BBa_B0010
     terminator      2332..2411
                     /label=BBa_B0010
     stem_loop       2427..2446
                     /label=T7 TE
     misc_feature    2453..2453
                     /label=stop
     polyA_site      2447..2460
                     /label=polya
     misc_feature    2420..2460
                     /label=BBa_B0012
     terminator      2420..2460
                     /label=BBa_B0012
     terminator      2332..2460
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagaca ggacctacta
       61 gatgagcagc gacagtgatc gtcagtgtcc tgtggatggc gatatagacc agcaggaaat
      121 gataccaagc aaaaaaaacg cggtgctggt ggatggcgtg gtgctgaacg ggccgaccac
      181 cgatgcgaaa gcgggcgaaa aattcgtcga ggaagcgtgc cgcctgatta tggaggaggt
      241 cgtcctcaaa gcgacagatg tgaacgaaaa agtgtgcgaa tggcgaccac ctgaacagct
      301 aaaacagcta cttgatctgg agatgcgcga tagcggcgaa ccgccacata aactgctgga
      361 actttgccgc gatgtgattc attatagcgt gaaaaccaac catccgcgat tttttaacca
      421 gctttatgcg ggcctggatt attatagcct ggtggcgcgc tttatgacag aagctcttaa
      481 ccccagcgtt tacacctatg aagtaagccc ggtgtttctc ctggtggaag aagcggtgct
      541 aaagaagatg atagagttca ttggctggaa agaaggcgat ggcattttta atccaggcgg
      601 aagcgtgagc aatatgtatg cgatgaacct ggcgcgttat aagtattgcc cggatattaa
      661 agaaaaaggg ctttcgggca gcccgcgtct catactgttt accagcgcgg aatgccacta
      721 tagcatgaag aaagcggcga gctttctggg cattggcaca gaaaatgtat gctttgtgga
      781 aaccgatgga cggggcaaaa tgataccaga agaattagaa aaacaggtgt ggcaggcgcg
      841 caaggaaggg gcggccccgt ttctggtgtg cgcgaccagc ggcacaaccg ttctgggcgc
      901 gtttgacccg ctggatgaaa ttgcggatat ttgcgaacgc catagcctgt ggcttcatgt
      961 tgatgcgagc tggggtggca gcgccctgat gagccgcaaa catcgcaaac tgctacatgg
     1021 cattcatcgc gcggatagcg tggcgtggaa cccgcataaa atgctcatgg caggcattca
     1081 gtgctgcgct ctgctggtga aagataaaag cgatctactg aaaaaatgtt atagcgctaa
     1141 ggcgagctat ctgtttcagc aggataaatt ttatgatgtg agctatgata cgggcgataa
     1201 gagcattcag tgtagccgcc gcccggatgc gtttaagttt tggatgacct ggaaagctct
     1261 gggaacgctg ggcctggaag aacgagtgaa ccgcgcgctt gcgctatctc gctatctggt
     1321 ggatgaaatt aaaaaacgcg aaggctttaa gctgctgatg gaacctgaat atgcgaacat
     1381 ttgcttttgg tatattcccc caagcctgcg cgaaatggaa gaaggcccgg aattttgggc
     1441 gaaactgaat ctggtggccc ctgcgataaa agaacgcatg atgaaaaaag gcagcctgat
     1501 gctgggctat cagccgcatc gcggcaaagt gaactttttt cgccaggtgg tgattagccc
     1561 acaggtgagc cgcgaagata tggatttcct gctcgacgag attgatctac tggggaagga
     1621 tatgatacta gagaaagaca ggacctacta gatgtcccac agcaagcagg cattagccga
     1681 caccatgaaa atgacatggc tgatggaagg tagcgtcaat gggcatgcat ttacgattga
     1741 aggtgagggc acggggaaac catatgaggg caaacagagc gggacgttcc gcgtgaccaa
     1801 aggtgggccg ctgccctttg ctttcgacat cgtagctcca acgttaaaat atggttttaa
     1861 gtgctttatg aaatatcccg cagacattcc ggattatttc aaactggcgt ttccggaggg
     1921 tttaacctac gatcgtaaaa ttgcgtttga agatggcggc tgtgcaacgg cgaccgtgga
     1981 aatgagtctg aagggtaata ctctggtcca caaaaccaac ttccaagggg gcaactttcc
     2041 tatcgatggc ccggtcatgc aaaaacgcac ccttgggtgg gagccgacga gtgaaaaaat
     2101 gacgccctgc gacgggatca ttaaaggcga taccattatg tacttaatgg tagagggtgg
     2161 caaaaccctc aaatgtcgct acgagaacaa ttatcgcgca aacaagcccg ttctcatgcc
     2221 gccgtcgcat tttgttgact tgcgtctgac ccgcactaac ctggacaaag agggcttggc
     2281 tttcaagctg gaagagtatg cggtggcgcg cgttctggag gtgtactaga gccaggcatc
     2341 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2401 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//