BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J61101 1 BBa_J61101 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A fix false false _95_ 0 483 95 In stock false N/A true John Anderson BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K2004012 1 BBa_K2004012 CSAD and reporter 2016-10-17T11:00:00Z 2016-10-18T01:03:58Z NCBI databank. Sequences taken from H. sapiens This device was designed to ease the identification of a functional enzymatic expression. A culture with a proper expression of the CSAD construct, will be pink because the reporter gene located downstream of this sequence which codifies to pink chromoprotein (spisPink). false false _2471_ 25099 25099 9 false Codon optimized for E. coli and sequences free of restrictions sites. false Marco V. Lincango Y. component2517659 1 BBa_J61101 component2517656 1 BBa_J23101 component2517660 1 BBa_K2004004 component2517667 1 BBa_B0015 component2517657 1 BBa_J61101 component2517658 1 BBa_K2004008 annotation2517658 1 BBa_K2004008 range2517658 1 62 1625 annotation2517660 1 BBa_K2004004 range2517660 1 1652 2323 annotation2517667 1 BBa_B0015 range2517667 1 2332 2460 annotation2517656 1 BBa_J23101 range2517656 1 1 35 annotation2517657 1 BBa_J61101 range2517657 1 44 55 annotation2517659 1 BBa_J61101 range2517659 1 1634 1645 BBa_K2004008 1 CSAD GADL1 Acidic Amino Acid Decarboxylase 2016-10-17T11:00:00Z 2016-10-18T11:34:57Z The sequence of this part was taken from NCBI databank, from the Homo sapiens genome sequence. GADL1 (Glutamate Decarboxylase Like 1) is a Protein Coding gene. Diseases associated with GADL1 include Gadl1-Related Altered Drug Metabolism. Among its related pathways are Pantothenate and CoA biosynthesis and Taurine and hypotaurine metabolism. GADL1 encodes a member of the group 2 decarboxylase family. A similar protein in rodents plays a role in multiple biological processes as the rate-limiting enzyme in taurine biosynthesis, catalyzing the decarboxylation of cysteine sulfinate to hypotaurine. The cysteine sulfinic acid decarboxylase enzyme (CSAD), also known as sulfoalanine decarboxylase, is an enzyme involved in the taurine biosynthesis. It is in charge of catalyzing the reaction that decarboxylates cysteine sulfinate generating hypotaurine and CO2. CSAD is a liase, and more specifically a carboxylase (Sumizu, K., 1962) false false _2471_ 25099 25099 9 false The sequence was cleaned up from restriction sites, to be able to use in a biobrick format. Furthermore, this part was codon optimized to E. coli. false Marco V. Lincango Y. BBa_K2004004 1 BBa_K2004004 spisPink: Pink chromoprotein optimized for E.coli 2016-10-17T11:00:00Z 2016-10-18T10:43:40Z This part is an improved version of BBa_K1684000, designed by BIOSINT_Mexico previous team. Chromoproteins are a collection of proteins that express pigments. This protein, expressed in Stylophora pistillata, was evolving independently from the rest of coral chromoproteins. The protein has a maximum absorption of 560 nm. This characteristic confers a blue shift by at least 14 nanometers in comparison to other known coral chromoproteins, inducing the production of pink color. This basic part can be used for whatever E. coli strain and is able to use with RFC 10 assembly. false false _2471_ 25099 25099 9 false The sequence was analyzed by softwars and we noticed some mistakes into the sequence to be part of the standardized biobrick library. This new version of the part contains all the specifications required to be a functional biobrick. false Marco V. Lincango Y. BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J61101_sequence 1 aaagacaggacc BBa_K2004004_sequence 1 atgtcccacagcaagcaggcattagccgacaccatgaaaatgacatggctgatggaaggtagcgtcaatgggcatgcatttacgattgaaggtgagggcacggggaaaccatatgagggcaaacagagcgggacgttccgcgtgaccaaaggtgggccgctgccctttgctttcgacatcgtagctccaacgttaaaatatggttttaagtgctttatgaaatatcccgcagacattccggattatttcaaactggcgtttccggagggtttaacctacgatcgtaaaattgcgtttgaagatggcggctgtgcaacggcgaccgtggaaatgagtctgaagggtaatactctggtccacaaaaccaacttccaagggggcaactttcctatcgatggcccggtcatgcaaaaacgcacccttgggtgggagccgacgagtgaaaaaatgacgccctgcgacgggatcattaaaggcgataccattatgtacttaatggtagagggtggcaaaaccctcaaatgtcgctacgagaacaattatcgcgcaaacaagcccgttctcatgccgccgtcgcattttgttgacttgcgtctgacccgcactaacctggacaaagagggcttggctttcaagctggaagagtatgcggtggcgcgcgttctggaggtg BBa_K2004012_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagacaggacctactagatgagcagcgacagtgatcgtcagtgtcctgtggatggcgatatagaccagcaggaaatgataccaagcaaaaaaaacgcggtgctggtggatggcgtggtgctgaacgggccgaccaccgatgcgaaagcgggcgaaaaattcgtcgaggaagcgtgccgcctgattatggaggaggtcgtcctcaaagcgacagatgtgaacgaaaaagtgtgcgaatggcgaccacctgaacagctaaaacagctacttgatctggagatgcgcgatagcggcgaaccgccacataaactgctggaactttgccgcgatgtgattcattatagcgtgaaaaccaaccatccgcgattttttaaccagctttatgcgggcctggattattatagcctggtggcgcgctttatgacagaagctcttaaccccagcgtttacacctatgaagtaagcccggtgtttctcctggtggaagaagcggtgctaaagaagatgatagagttcattggctggaaagaaggcgatggcatttttaatccaggcggaagcgtgagcaatatgtatgcgatgaacctggcgcgttataagtattgcccggatattaaagaaaaagggctttcgggcagcccgcgtctcatactgtttaccagcgcggaatgccactatagcatgaagaaagcggcgagctttctgggcattggcacagaaaatgtatgctttgtggaaaccgatggacggggcaaaatgataccagaagaattagaaaaacaggtgtggcaggcgcgcaaggaaggggcggccccgtttctggtgtgcgcgaccagcggcacaaccgttctgggcgcgtttgacccgctggatgaaattgcggatatttgcgaacgccatagcctgtggcttcatgttgatgcgagctggggtggcagcgccctgatgagccgcaaacatcgcaaactgctacatggcattcatcgcgcggatagcgtggcgtggaacccgcataaaatgctcatggcaggcattcagtgctgcgctctgctggtgaaagataaaagcgatctactgaaaaaatgttatagcgctaaggcgagctatctgtttcagcaggataaattttatgatgtgagctatgatacgggcgataagagcattcagtgtagccgccgcccggatgcgtttaagttttggatgacctggaaagctctgggaacgctgggcctggaagaacgagtgaaccgcgcgcttgcgctatctcgctatctggtggatgaaattaaaaaacgcgaaggctttaagctgctgatggaacctgaatatgcgaacatttgcttttggtatattcccccaagcctgcgcgaaatggaagaaggcccggaattttgggcgaaactgaatctggtggcccctgcgataaaagaacgcatgatgaaaaaaggcagcctgatgctgggctatcagccgcatcgcggcaaagtgaacttttttcgccaggtggtgattagcccacaggtgagccgcgaagatatggatttcctgctcgacgagattgatctactggggaaggatatgatactagagaaagacaggacctactagatgtcccacagcaagcaggcattagccgacaccatgaaaatgacatggctgatggaaggtagcgtcaatgggcatgcatttacgattgaaggtgagggcacggggaaaccatatgagggcaaacagagcgggacgttccgcgtgaccaaaggtgggccgctgccctttgctttcgacatcgtagctccaacgttaaaatatggttttaagtgctttatgaaatatcccgcagacattccggattatttcaaactggcgtttccggagggtttaacctacgatcgtaaaattgcgtttgaagatggcggctgtgcaacggcgaccgtggaaatgagtctgaagggtaatactctggtccacaaaaccaacttccaagggggcaactttcctatcgatggcccggtcatgcaaaaacgcacccttgggtgggagccgacgagtgaaaaaatgacgccctgcgacgggatcattaaaggcgataccattatgtacttaatggtagagggtggcaaaaccctcaaatgtcgctacgagaacaattatcgcgcaaacaagcccgttctcatgccgccgtcgcattttgttgacttgcgtctgacccgcactaacctggacaaagagggcttggctttcaagctggaagagtatgcggtggcgcgcgttctggaggtgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2004008_sequence 1 atgagcagcgacagtgatcgtcagtgtcctgtggatggcgatatagaccagcaggaaatgataccaagcaaaaaaaacgcggtgctggtggatggcgtggtgctgaacgggccgaccaccgatgcgaaagcgggcgaaaaattcgtcgaggaagcgtgccgcctgattatggaggaggtcgtcctcaaagcgacagatgtgaacgaaaaagtgtgcgaatggcgaccacctgaacagctaaaacagctacttgatctggagatgcgcgatagcggcgaaccgccacataaactgctggaactttgccgcgatgtgattcattatagcgtgaaaaccaaccatccgcgattttttaaccagctttatgcgggcctggattattatagcctggtggcgcgctttatgacagaagctcttaaccccagcgtttacacctatgaagtaagcccggtgtttctcctggtggaagaagcggtgctaaagaagatgatagagttcattggctggaaagaaggcgatggcatttttaatccaggcggaagcgtgagcaatatgtatgcgatgaacctggcgcgttataagtattgcccggatattaaagaaaaagggctttcgggcagcccgcgtctcatactgtttaccagcgcggaatgccactatagcatgaagaaagcggcgagctttctgggcattggcacagaaaatgtatgctttgtggaaaccgatggacggggcaaaatgataccagaagaattagaaaaacaggtgtggcaggcgcgcaaggaaggggcggccccgtttctggtgtgcgcgaccagcggcacaaccgttctgggcgcgtttgacccgctggatgaaattgcggatatttgcgaacgccatagcctgtggcttcatgttgatgcgagctggggtggcagcgccctgatgagccgcaaacatcgcaaactgctacatggcattcatcgcgcggatagcgtggcgtggaacccgcataaaatgctcatggcaggcattcagtgctgcgctctgctggtgaaagataaaagcgatctactgaaaaaatgttatagcgctaaggcgagctatctgtttcagcaggataaattttatgatgtgagctatgatacgggcgataagagcattcagtgtagccgccgcccggatgcgtttaagttttggatgacctggaaagctctgggaacgctgggcctggaagaacgagtgaaccgcgcgcttgcgctatctcgctatctggtggatgaaattaaaaaacgcgaaggctttaagctgctgatggaacctgaatatgcgaacatttgcttttggtatattcccccaagcctgcgcgaaatggaagaaggcccggaattttgggcgaaactgaatctggtggcccctgcgataaaagaacgcatgatgaaaaaaggcagcctgatgctgggctatcagccgcatcgcggcaaagtgaacttttttcgccaggtggtgattagcccacaggtgagccgcgaagatatggatttcctgctcgacgagattgatctactggggaaggatatga BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z