LOCUS       BBa_K2004013             914 bp    DNA     linear       15-Nov-2024
DEFINITION  spisPink poly his tag
ACCESSION   BBa_K2004013
VERSION     BBa_K2004013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..55
                     /label=BBa_J61101
     misc_feature    62..733
                     /label=BBa_K2004004
     misc_feature    742..771
                     /label=10x-Histidine Tag
     misc_feature    772..774
                     /label=Stop
     misc_feature    775..777
                     /label=Stop
     misc_feature    742..777
                     /label=BBa_K844000
     stem_loop       797..840
                     /label=stem_loop
     misc_feature    786..865
                     /label=BBa_B0010
     terminator      786..865
                     /label=BBa_B0010
     stem_loop       881..900
                     /label=T7 TE
     misc_feature    907..907
                     /label=stop
     polyA_site      901..914
                     /label=polya
     misc_feature    874..914
                     /label=BBa_B0012
     terminator      874..914
                     /label=BBa_B0012
     terminator      786..914
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagaca ggacctacta
       61 gatgtcccac agcaagcagg cattagccga caccatgaaa atgacatggc tgatggaagg
      121 tagcgtcaat gggcatgcat ttacgattga aggtgagggc acggggaaac catatgaggg
      181 caaacagagc gggacgttcc gcgtgaccaa aggtgggccg ctgccctttg ctttcgacat
      241 cgtagctcca acgttaaaat atggttttaa gtgctttatg aaatatcccg cagacattcc
      301 ggattatttc aaactggcgt ttccggaggg tttaacctac gatcgtaaaa ttgcgtttga
      361 agatggcggc tgtgcaacgg cgaccgtgga aatgagtctg aagggtaata ctctggtcca
      421 caaaaccaac ttccaagggg gcaactttcc tatcgatggc ccggtcatgc aaaaacgcac
      481 ccttgggtgg gagccgacga gtgaaaaaat gacgccctgc gacgggatca ttaaaggcga
      541 taccattatg tacttaatgg tagagggtgg caaaaccctc aaatgtcgct acgagaacaa
      601 ttatcgcgca aacaagcccg ttctcatgcc gccgtcgcat tttgttgact tgcgtctgac
      661 ccgcactaac ctggacaaag agggcttggc tttcaagctg gaagagtatg cggtggcgcg
      721 cgttctggag gtgtactaga gcatcatcac catcaccacc atcatcacca ttaataatac
      781 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      841 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      901 tttctgcgtt tata
//