BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J61101 1 BBa_J61101 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A fix false false _95_ 0 483 95 In stock false N/A true John Anderson BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K2004013 1 BBa_K2004013 spisPink poly his tag 2016-10-17T11:00:00Z 2016-10-18T01:09:27Z Expressed in Stylophora pistillata, was evolving independently from the rest of coral chromoproteins. In this way, a brief step to confirm the chromoprotein expression is purifying it, so to facilitate this process we fuse a improved version of the sequence (BBa_K2004004) that codifies to pink chromoprotein with another registered part, the poly his tag (BBa_K844000). The last version of this chromoprotein(BBa_K1684000), designed by the previous Biosint team, was analyzed according to its sequence and all the parameter that must meet to be part of a standardized biobrick. This device (BBa_K2004013) allows to test the fusion, because contains a constitutive promoter and a RBS upstream and the double terminator downstream. false false _2471_ 25099 25099 9 false It is a improved and correct version of the protein spispink, to be able to use like a biobrick. false Marco V. Lincango Y. component2517738 1 BBa_J61101 component2517739 1 BBa_K2004004 component2517737 1 BBa_J23101 component2517750 1 BBa_B0015 component2517743 1 BBa_K844000 annotation2517739 1 BBa_K2004004 range2517739 1 62 733 annotation2517743 1 BBa_K844000 range2517743 1 742 777 annotation2517750 1 BBa_B0015 range2517750 1 786 914 annotation2517738 1 BBa_J61101 range2517738 1 44 55 annotation2517737 1 BBa_J23101 range2517737 1 1 35 BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206608 1 Stop range2206608 1 31 33 annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206609 1 Stop range2206609 1 34 36 BBa_K2004004 1 BBa_K2004004 spisPink: Pink chromoprotein optimized for E.coli 2016-10-17T11:00:00Z 2016-10-18T10:43:40Z This part is an improved version of BBa_K1684000, designed by BIOSINT_Mexico previous team. Chromoproteins are a collection of proteins that express pigments. This protein, expressed in Stylophora pistillata, was evolving independently from the rest of coral chromoproteins. The protein has a maximum absorption of 560 nm. This characteristic confers a blue shift by at least 14 nanometers in comparison to other known coral chromoproteins, inducing the production of pink color. This basic part can be used for whatever E. coli strain and is able to use with RFC 10 assembly. false false _2471_ 25099 25099 9 false The sequence was analyzed by softwars and we noticed some mistakes into the sequence to be part of the standardized biobrick library. This new version of the part contains all the specifications required to be a functional biobrick. false Marco V. Lincango Y. BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K2004013_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagacaggacctactagatgtcccacagcaagcaggcattagccgacaccatgaaaatgacatggctgatggaaggtagcgtcaatgggcatgcatttacgattgaaggtgagggcacggggaaaccatatgagggcaaacagagcgggacgttccgcgtgaccaaaggtgggccgctgccctttgctttcgacatcgtagctccaacgttaaaatatggttttaagtgctttatgaaatatcccgcagacattccggattatttcaaactggcgtttccggagggtttaacctacgatcgtaaaattgcgtttgaagatggcggctgtgcaacggcgaccgtggaaatgagtctgaagggtaatactctggtccacaaaaccaacttccaagggggcaactttcctatcgatggcccggtcatgcaaaaacgcacccttgggtgggagccgacgagtgaaaaaatgacgccctgcgacgggatcattaaaggcgataccattatgtacttaatggtagagggtggcaaaaccctcaaatgtcgctacgagaacaattatcgcgcaaacaagcccgttctcatgccgccgtcgcattttgttgacttgcgtctgacccgcactaacctggacaaagagggcttggctttcaagctggaagagtatgcggtggcgcgcgttctggaggtgtactagagcatcatcaccatcaccaccatcatcaccattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J61101_sequence 1 aaagacaggacc BBa_K2004004_sequence 1 atgtcccacagcaagcaggcattagccgacaccatgaaaatgacatggctgatggaaggtagcgtcaatgggcatgcatttacgattgaaggtgagggcacggggaaaccatatgagggcaaacagagcgggacgttccgcgtgaccaaaggtgggccgctgccctttgctttcgacatcgtagctccaacgttaaaatatggttttaagtgctttatgaaatatcccgcagacattccggattatttcaaactggcgtttccggagggtttaacctacgatcgtaaaattgcgtttgaagatggcggctgtgcaacggcgaccgtggaaatgagtctgaagggtaatactctggtccacaaaaccaacttccaagggggcaactttcctatcgatggcccggtcatgcaaaaacgcacccttgggtgggagccgacgagtgaaaaaatgacgccctgcgacgggatcattaaaggcgataccattatgtacttaatggtagagggtggcaaaaccctcaaatgtcgctacgagaacaattatcgcgcaaacaagcccgttctcatgccgccgtcgcattttgttgacttgcgtctgacccgcactaacctggacaaagagggcttggctttcaagctggaagagtatgcggtggcgcgcgttctggaggtg BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z