BBa_K2005091
1
BBa_K2005091
CAN1, UV-maximized
2016-10-17T11:00:00Z
2016-10-18T08:04:15Z
Modified from S. cerevisiae genomic sequences
Coding sequence for CAN1 gene from yeast (Saccharomyces cerevisiae) with its sequence modified by our algorithm to maximize its susceptibility to mutation. This sequence has sufficient flanking homology regions to allow for integration into yeast, and features base modifications to accommodate CRISPR homology-directed repair. CAN1 codes for an arginine permease that also confers susceptibility to the drug canavanine.
We designed K2005090 as part of an in vivo selection system, where mutant cells could be positively enriched using canavanine to measure rates of mutation with our optimized genes.
false
false
_2472_
19415
19415
9
false
To generate this artificial DNA sequence, we employed our custom-made algorithm for modifying gene sequences to introduce mutagenic sites. These sites were identified based on prior research on DNA irradiation, where dipyrimidine motifs as particularly vulnerable. At these motifs, synonymous codon substitutions were made to make replacements for ones with higher mutation risks. In addition, our algorithm used heuristics to factor in the effect different codons have on gene expression to ensure that the gene not only produced the same protein but produced it at similar levels. Overall we could increase the predicted number of UV-mutation sites by 63%. This sequence also has 50 bp homology arms for genomic integration, and SNPs at CRISPR target sites.
false
Jarrod Shilts
BBa_K2005091_sequence
1
ggcatagcaatgacaaattcaaaagaagacgccgacatagaggagaagcatatgtataatgaaccagtcacaactctttttcatgatgttgaagcttctcaaactcatcataggagaggatcaattcctttgaaagatgagaaatcaaaagaactttatcctttgagatcttttccgacaagagtcaatggagaagatactttttctatggaagacggaatcggagacgaggatgaaggtgaagttcaaaatgcagaagtgaagagagaactgaaacaaagacatattggaatgatcgctcttggaggaactatcggaacaggattattcatcggactttctactcctttgacaaatgcaggaccagtgggagctttgatttcttatctttttatgggatctcttgcttattcagtcactcaatctcttggtgaaatggcaaccttcattcctgtgacttcttctttcacagttttctctcaaagatttctttctcctgctttcggagcagcaaacggatacatgtactggttttcctgggcaatcactttcgctcttgaactttctgtcgtcggtcaagtcatccaattttggacttataaagttcctcttgcagcctggatttctattttctgggtcatcattacaattatgaacttatttcctgtcaagtattatggagagttcgagttttgggtcgcttcaatcaaagttttagcaattatcggattcttgatttattgcttttgcatggtttgtggagctggagtgacaggaccagtaggatttcgttattggagaaatccaggcgcttggggaccaggaataatctctaaggataaaaacgaaggaagattccttggatgggtttcttctttaatcaatgccgcttttacatttcagggaacagaacttgtcggaattacagcaggcgaagcagctaatcctagaaaatctgtcccgagagctattaaaaaagttgtcttcagaattttaaccttttacatcggaagtcttttgtttatcggcttgttagttccctataacgatccgaaattgactcaatcaacttcttatgtttcaacttctcctttcataatcgccatcgaaaattcaggaaccaaagtccttcctcatatattcaacgcagtgattctaacaacaatcatctcagctgctaactctaatatttacgttggatcaagaattttgtttggactttccaaaaataaattagctcctaaatttttgtcaaggactactaaaggaggagttccctatattgcagtcttcgtaactgctgccttcggagctcttgcctatatggaaacttcaaccggaggagataaagttttcgagtggcttttgaatatcacaggagtcgcaggatttttcgcttggttgtttatctctatctctcacatcaggttcatgcaagctcttaaatatagaggaatctctagggacgagcttccatttaaggcaaagttgatgccgggtctagcttattacgccgctaccttcatgaccataataataattatacaaggattcactgcatttgctcctaagtttaacggagtcagtttcgcagccgcctatatctccatcttccttttcttagctgtctggatactattccagtgtatctttagatgtaggttcatctggaagatcggcgatgtcgacatcgactctgatagaagagacatcgaagcaatcgtttgggaagatcacgaacccaagaccttctgggataagttttggaacgtcgttgcttga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z