LOCUS       BBa_K2007000            2009 bp    DNA     linear       16-Feb-2025
DEFINITION  Constitutive promote expressing GFP and Lac promoter expressing 
            FadR gene
ACCESSION   BBa_K2007000
VERSION     BBa_K2007000.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..781
                     /label=GFP protein
     CDS             62..781
                     /label=BBa_E0040
     stem_loop       801..844
                     /label=stem_loop
     misc_feature    790..869
                     /label=BBa_B0010
     terminator      790..869
                     /label=BBa_B0010
     stem_loop       885..904
                     /label=T7 TE
     misc_feature    911..911
                     /label=stop
     polyA_site      905..918
                     /label=polya
     misc_feature    878..918
                     /label=BBa_B0012
     terminator      878..918
                     /label=BBa_B0012
     terminator      790..918
                     /label=BBa_B0015
     CDS             927..1014
                     /label=end of LacI coding region (inactive)
     misc_feature    927..1126
                     /label=BBa_R0010
     misc_feature    1015..1052
                     /label=CAP binding site
     promoter        1063..1068
                     /label=-35
     promoter        1087..1092
                     /label=-10
     misc_feature    1092..1126
                     /label=LacI binding site
     misc_feature    1099..1099
                     /label=start
     promoter        927..1126
                     /label=BBa_R0010
     misc_feature    1139..1142
                     /label=conserved
     RBS             1135..1146
                     /label=BBa_B0034
     CDS             1153..1872
                     /label=FadR
     CDS             1153..1872
                     /label=BBa_K817001
     stem_loop       1892..1935
                     /label=stem_loop
     misc_feature    1881..1960
                     /label=BBa_B0010
     terminator      1881..1960
                     /label=BBa_B0010
     stem_loop       1976..1995
                     /label=T7 TE
     misc_feature    2002..2002
                     /label=stop
     polyA_site      1996..2009
                     /label=polya
     misc_feature    1969..2009
                     /label=BBa_B0012
     terminator      1969..2009
                     /label=BBa_B0012
     terminator      1881..2009
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc
      961 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1021 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     1081 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag
     1141 gagaaatact agatggtcat taaggcgcaa agcccggcgg gtttcgcgga agagtacatt
     1201 attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc agaacgtgaa
     1261 ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca gcgtctggca
     1321 cgagatggct ggttgaccat tcaacatggc aagccgacga aggtgaataa tttctgggaa
     1381 acttccggtt taaatatcct tgaaacactg gcgcgactgg atcacgaaag tgtgccgcag
     1441 cttattgata atttgctgtc ggtgcgtacc aatatttcca ctatttttat tcgcaccgcg
     1501 tttcgtcagc atcccgataa agcgcaggaa gtgctggcta ccgctaatga agtggccgat
     1561 cacgccgatg cctttgccga gctggattac aacatattcc gcggcctggc gtttgcttcc
     1621 ggcaacccga tttacggtct gattcttaac gggatgaaag ggctgtatac gcgtattggt
     1681 cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta ccacaaactg
     1741 tcggcgttgt gcagtgaagg cgcgcacgat caggtgtacg aaacagtgcg tcgctatggg
     1801 catgagagtg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga tttagccatt
     1861 caggggcgat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1921 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1981 accttcgggt gggcctttct gcgtttata
//