LOCUS       BBa_K2007001            2046 bp    DNA     linear       22-Nov-2024
DEFINITION  pLR promoter expressing GFP and Lac promoter expressing FadR gene
ACCESSION   BBa_K2007001
VERSION     BBa_K2007001.1  
FEATURES             Location/Qualifiers
     promoter        1..72
                     /label=PfadBA
     promoter        1..72
                     /label=BBa_K817002
     misc_feature    85..88
                     /label=conserved
     RBS             81..92
                     /label=BBa_B0034
     CDS             99..818
                     /label=GFP protein
     CDS             99..818
                     /label=BBa_E0040
     stem_loop       838..881
                     /label=stem_loop
     misc_feature    827..906
                     /label=BBa_B0010
     terminator      827..906
                     /label=BBa_B0010
     stem_loop       922..941
                     /label=T7 TE
     misc_feature    948..948
                     /label=stop
     polyA_site      942..955
                     /label=polya
     misc_feature    915..955
                     /label=BBa_B0012
     terminator      915..955
                     /label=BBa_B0012
     terminator      827..955
                     /label=BBa_B0015
     CDS             964..1051
                     /label=end of LacI coding region (inactive)
     misc_feature    964..1163
                     /label=BBa_R0010
     misc_feature    1052..1089
                     /label=CAP binding site
     promoter        1100..1105
                     /label=-35
     promoter        1124..1129
                     /label=-10
     misc_feature    1129..1163
                     /label=LacI binding site
     misc_feature    1136..1136
                     /label=start
     promoter        964..1163
                     /label=BBa_R0010
     misc_feature    1176..1179
                     /label=conserved
     RBS             1172..1183
                     /label=BBa_B0034
     CDS             1190..1909
                     /label=FadR
     CDS             1190..1909
                     /label=BBa_K817001
     stem_loop       1929..1972
                     /label=stem_loop
     misc_feature    1918..1997
                     /label=BBa_B0010
     terminator      1918..1997
                     /label=BBa_B0010
     stem_loop       2013..2032
                     /label=T7 TE
     misc_feature    2039..2039
                     /label=stop
     polyA_site      2033..2046
                     /label=polya
     misc_feature    2006..2046
                     /label=BBa_B0012
     terminator      2006..2046
                     /label=BBa_B0012
     terminator      1918..2046
                     /label=BBa_B0015
ORIGIN
        1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag
       61 gacgcactga cctactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt
      121 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      181 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      241 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
      301 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      361 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      421 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      481 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      541 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      601 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
      661 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
      721 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
      781 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
      841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      901 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
      961 gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1021 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     1081 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1141 tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tggtcattaa
     1201 ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct ggaataaccg
     1261 cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa ttggcgtaac
     1321 gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt tgaccattca
     1381 acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa atatccttga
     1441 aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt tgctgtcggt
     1501 gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc ccgataaagc
     1561 gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct ttgccgagct
     1621 ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt acggtctgat
     1681 tcttaacggg atgaaagggc tgtatacgcg tattggtcgt cactatttcg ccaatccgga
     1741 agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca gtgaaggcgc
     1801 gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg agatttggca
     1861 ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat actagagcca
     1921 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1981 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     2041 tttata
//