LOCUS       BBa_K2007005            2929 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive promoter expressing DmJHAMT and GFP, Lac promoter 
            expressing FadR
ACCESSION   BBa_K2007005
VERSION     BBa_K2007005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..955
                     /label=BBa_K2007002
     misc_feature    968..971
                     /label=conserved
     RBS             964..975
                     /label=BBa_B0034
     CDS             982..1701
                     /label=GFP protein
     CDS             982..1701
                     /label=BBa_E0040
     stem_loop       1721..1764
                     /label=stem_loop
     misc_feature    1710..1789
                     /label=BBa_B0010
     terminator      1710..1789
                     /label=BBa_B0010
     stem_loop       1805..1824
                     /label=T7 TE
     misc_feature    1831..1831
                     /label=stop
     polyA_site      1825..1838
                     /label=polya
     misc_feature    1798..1838
                     /label=BBa_B0012
     terminator      1798..1838
                     /label=BBa_B0012
     terminator      1710..1838
                     /label=BBa_B0015
     CDS             1847..1934
                     /label=end of LacI coding region (inactive)
     misc_feature    1847..2046
                     /label=BBa_R0010
     misc_feature    1935..1972
                     /label=CAP binding site
     promoter        1983..1988
                     /label=-35
     promoter        2007..2012
                     /label=-10
     misc_feature    2012..2046
                     /label=LacI binding site
     misc_feature    2019..2019
                     /label=start
     promoter        1847..2046
                     /label=BBa_R0010
     misc_feature    2059..2062
                     /label=conserved
     RBS             2055..2066
                     /label=BBa_B0034
     CDS             2073..2792
                     /label=FadR
     CDS             2073..2792
                     /label=BBa_K817001
     stem_loop       2812..2855
                     /label=stem_loop
     misc_feature    2801..2880
                     /label=BBa_B0010
     terminator      2801..2880
                     /label=BBa_B0010
     stem_loop       2896..2915
                     /label=T7 TE
     misc_feature    2922..2922
                     /label=stop
     polyA_site      2916..2929
                     /label=polya
     misc_feature    2889..2929
                     /label=BBa_B0012
     terminator      2889..2929
                     /label=BBa_B0012
     terminator      2801..2929
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaatcag gcctctctat atcagcacgc caaccaggtg caaaggcacg atgccaaatt
      121 gattctggac gagttcgcat cgaccatgca gtggcgatcc gatggagagg atgcactcct
      181 ggatgtgggt tcaggatccg gtaacgtgct catggacttc gtaaagccac tgctcccgat
      241 tcgtggacaa ctggtgggca cagacatctc cagccagatg gtgcactatg ccagtaagca
      301 ttaccagcga gaggagcgca ccagattcca ggttctggac attggatgcg agcgactgcc
      361 tgaggagctt agcggcagat ttgaccatgt cacctcgttc tactgcctcc attgggtgca
      421 aaatctgaaa ggagctctcg gaaatatcta caatcttctg aagcccgaag gtggcgactg
      481 cctcctggcc tttttggcct ccaatccggt ttacgaagtc tataagattc ttaaaacgaa
      541 tgacaagtgg tcgactttta tgcaggatgt ggagaacttc atatccccac ttcactacag
      601 tctaagtcct ggcgaggagt tcagtcagtt gttgaacgat gtgggtttcg tgcaacacaa
      661 tgtggaaatt cgaaacgaag tgtttgttta tgaaggcgtg aggacactga aagataatgt
      721 aaaggccatt tgtccttttc ttgagcgaat gccagcagat ttgcatgaac agttcctgga
      781 tgacttcata gacattgtta tatccatgaa tttgcagcaa ggtgaaaata atgaggatca
      841 aaagttccta tctccctata aattggtggt ggcctatgct cgcaagactc ctgaatttgt
      901 gaataatgtt ttcctggagc ctacacatca aaacttggtt aagggaataa attaatacta
      961 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
     1021 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     1081 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     1141 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     1201 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1261 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     1321 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1381 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1441 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     1501 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1561 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1621 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1681 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1741 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1801 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat acgcaaaccg
     1861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1921 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     1981 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     2041 cacacatact agagaaagag gagaaatact agatggtcat taaggcgcaa agcccggcgg
     2101 gtttcgcgga agagtacatt attgaaagta tctggaataa ccgcttccct cccgggacta
     2161 ttttgcccgc agaacgtgaa ctttcagaat taattggcgt aacgcgtact acgttacgtg
     2221 aagtgttaca gcgtctggca cgagatggct ggttgaccat tcaacatggc aagccgacga
     2281 aggtgaataa tttctgggaa acttccggtt taaatatcct tgaaacactg gcgcgactgg
     2341 atcacgaaag tgtgccgcag cttattgata atttgctgtc ggtgcgtacc aatatttcca
     2401 ctatttttat tcgcaccgcg tttcgtcagc atcccgataa agcgcaggaa gtgctggcta
     2461 ccgctaatga agtggccgat cacgccgatg cctttgccga gctggattac aacatattcc
     2521 gcggcctggc gtttgcttcc ggcaacccga tttacggtct gattcttaac gggatgaaag
     2581 ggctgtatac gcgtattggt cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc
     2641 tgggcttcta ccacaaactg tcggcgttgt gcagtgaagg cgcgcacgat caggtgtacg
     2701 aaacagtgcg tcgctatggg catgagagtg gcgagatttg gcaccggatg cagaaaaatc
     2761 tgccgggtga tttagccatt caggggcgat aatactagag ccaggcatca aataaaacga
     2821 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2881 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//