LOCUS BBa_K2007006 2966 bp DNA linear 22-Nov-2024 DEFINITION pLR promoter expressing DmJHAMT and GFP, Lac promoter expressing FadR ACCESSION BBa_K2007006 VERSION BBa_K2007006.1 FEATURES Location/Qualifiers promoter 1..72 /label=PfadBA promoter 1..72 /label=BBa_K817002 misc_feature 85..88 /label=conserved RBS 81..92 /label=BBa_B0034 CDS 99..992 /label=BBa_K2007002 misc_feature 1005..1008 /label=conserved RBS 1001..1012 /label=BBa_B0034 CDS 1019..1738 /label=GFP protein CDS 1019..1738 /label=BBa_E0040 stem_loop 1758..1801 /label=stem_loop misc_feature 1747..1826 /label=BBa_B0010 terminator 1747..1826 /label=BBa_B0010 stem_loop 1842..1861 /label=T7 TE misc_feature 1868..1868 /label=stop polyA_site 1862..1875 /label=polya misc_feature 1835..1875 /label=BBa_B0012 terminator 1835..1875 /label=BBa_B0012 terminator 1747..1875 /label=BBa_B0015 CDS 1884..1971 /label=end of LacI coding region (inactive) misc_feature 1884..2083 /label=BBa_R0010 misc_feature 1972..2009 /label=CAP binding site promoter 2020..2025 /label=-35 promoter 2044..2049 /label=-10 misc_feature 2049..2083 /label=LacI binding site misc_feature 2056..2056 /label=start promoter 1884..2083 /label=BBa_R0010 misc_feature 2096..2099 /label=conserved RBS 2092..2103 /label=BBa_B0034 CDS 2110..2829 /label=FadR CDS 2110..2829 /label=BBa_K817001 stem_loop 2849..2892 /label=stem_loop misc_feature 2838..2917 /label=BBa_B0010 terminator 2838..2917 /label=BBa_B0010 stem_loop 2933..2952 /label=T7 TE misc_feature 2959..2959 /label=stop polyA_site 2953..2966 /label=polya misc_feature 2926..2966 /label=BBa_B0012 terminator 2926..2966 /label=BBa_B0012 terminator 2838..2966 /label=BBa_B0015 ORIGIN 1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag 61 gacgcactga cctactagag aaagaggaga aatactagat gaatcaggcc tctctatatc 121 agcacgccaa ccaggtgcaa aggcacgatg ccaaattgat tctggacgag ttcgcatcga 181 ccatgcagtg gcgatccgat ggagaggatg cactcctgga tgtgggttca ggatccggta 241 acgtgctcat ggacttcgta aagccactgc tcccgattcg tggacaactg gtgggcacag 301 acatctccag ccagatggtg cactatgcca gtaagcatta ccagcgagag gagcgcacca 361 gattccaggt tctggacatt ggatgcgagc gactgcctga ggagcttagc ggcagatttg 421 accatgtcac ctcgttctac tgcctccatt gggtgcaaaa tctgaaagga gctctcggaa 481 atatctacaa tcttctgaag cccgaaggtg gcgactgcct cctggccttt ttggcctcca 541 atccggttta cgaagtctat aagattctta aaacgaatga caagtggtcg acttttatgc 601 aggatgtgga gaacttcata tccccacttc actacagtct aagtcctggc gaggagttca 661 gtcagttgtt gaacgatgtg ggtttcgtgc aacacaatgt ggaaattcga aacgaagtgt 721 ttgtttatga aggcgtgagg acactgaaag ataatgtaaa ggccatttgt ccttttcttg 781 agcgaatgcc agcagatttg catgaacagt tcctggatga cttcatagac attgttatat 841 ccatgaattt gcagcaaggt gaaaataatg aggatcaaaa gttcctatct ccctataaat 901 tggtggtggc ctatgctcgc aagactcctg aatttgtgaa taatgttttc ctggagccta 961 cacatcaaaa cttggttaag ggaataaatt aatactagag aaagaggaga aatactagat 1021 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga 1081 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa 1141 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt 1201 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca 1261 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa 1321 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa 1381 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt 1441 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat 1501 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca 1561 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct 1621 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct 1681 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata 1741 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1801 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1861 ctttctgcgt ttatatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat 1921 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 1981 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 2041 tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga gaaagaggag 2101 aaatactaga tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt 2161 gaaagtatct ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt 2221 tcagaattaa ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga 2281 gatggctggt tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact 2341 tccggtttaa atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt 2401 attgataatt tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt 2461 cgtcagcatc ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac 2521 gccgatgcct ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc 2581 aacccgattt acggtctgat tcttaacggg atgaaagggc tgtatacgcg tattggtcgt 2641 cactatttcg ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg 2701 gcgttgtgca gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat 2761 gagagtggcg agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag 2821 gggcgataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2881 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 2941 ttcgggtggg cctttctgcg tttata //