BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961224
1
-35
range1961224
1
137
142
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961227
1
start
range1961227
1
173
173
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K817001
1
BBa_K817001
FadR
2012-09-17T11:00:00Z
2015-05-08T01:13:28Z
E.coli genomic sequence
It's a repressor that E.coli use in sensing surrounding fatty acid concentration.
false
false
_1075_
0
13572
9
In stock
false
N/A
false
Tian-Shyang Shoung
annotation2187738
1
FadR
range2187738
1
1
720
BBa_K2007006
1
BBa_K2007006
pLR promoter expressing DmJHAMT and GFP, Lac promoter expressing FadR
2016-10-09T11:00:00Z
2016-10-10T09:59:44Z
iGEM library and IDT Gene Black
This circuit expresses GFP and a Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase (DmJHAMT) under a fatty acid concentration dependent manner. It also expresses FadR under the Lac promoter.
false
false
_2474_
27805
27805
9
false
N/A
false
Alina Khzanyan
component2491698
1
BBa_B0034
component2491711
1
BBa_R0010
component2491721
1
BBa_K817001
component2491703
1
BBa_E0040
component2491710
1
BBa_B0015
component2491719
1
BBa_B0034
component2491696
1
BBa_K817002
component2491728
1
BBa_B0015
component2491699
1
BBa_K2007002
component2491701
1
BBa_B0034
annotation2491703
1
BBa_E0040
range2491703
1
1019
1738
annotation2491699
1
BBa_K2007002
range2491699
1
99
992
annotation2491710
1
BBa_B0015
range2491710
1
1747
1875
annotation2491696
1
BBa_K817002
range2491696
1
1
72
annotation2491711
1
BBa_R0010
range2491711
1
1884
2083
annotation2491698
1
BBa_B0034
range2491698
1
81
92
annotation2491728
1
BBa_B0015
range2491728
1
2838
2966
annotation2491701
1
BBa_B0034
range2491701
1
1001
1012
annotation2491721
1
BBa_K817001
range2491721
1
2110
2829
annotation2491719
1
BBa_B0034
range2491719
1
2092
2103
BBa_K817002
1
BBa_K817002
PfadBA
2012-09-17T11:00:00Z
2015-05-08T01:13:28Z
de novo synthesis
Released HQ 2013
Fatty acid sensitive promoter
false
false
_1075_
0
13572
9
In stock
false
N/A
false
Yi-Te Lee, Chieh-Mei Wang
annotation2197686
1
PfadBA
range2197686
1
1
72
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K2007002
1
BBa_K2007002
Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase (DmJHAMT)
2016-10-09T11:00:00Z
2016-10-10T09:27:14Z
Sherkhanov, S., Korman, T. P., Clarke, S. G., & Bowie, J. U. (2016). Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme. Scientific reports, 6.
This is a coding sequence of a Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase (DmJHAMT) which catalyzed the esterification of FFAs and is specific to medium chain FFAs.
false
false
_2474_
27805
27805
9
Not in stock
false
We introduced two silent mutations to eliminate two Pst1 cut sites by changing the base pairs at position 72 from A to G and at position 558 from A to G so that we could use this part in a standard Bio Brick format.
false
Alina Khzanyan
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K817001_sequence
1
atggtcattaaggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactattttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggcacgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatccttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttccactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggccgatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacggtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtctggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgctatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2007002_sequence
1
atgaatcaggcctctctatatcagcacgccaaccaggtgcaaaggcacgatgccaaattgattctggacgagttcgcatcgaccatgcagtggcgatccgatggagaggatgcactcctggatgtgggttcaggatccggtaacgtgctcatggacttcgtaaagccactgctcccgattcgtggacaactggtgggcacagacatctccagccagatggtgcactatgccagtaagcattaccagcgagaggagcgcaccagattccaggttctggacattggatgcgagcgactgcctgaggagcttagcggcagatttgaccatgtcacctcgttctactgcctccattgggtgcaaaatctgaaaggagctctcggaaatatctacaatcttctgaagcccgaaggtggcgactgcctcctggcctttttggcctccaatccggtttacgaagtctataagattcttaaaacgaatgacaagtggtcgacttttatgcaggatgtggagaacttcatatccccacttcactacagtctaagtcctggcgaggagttcagtcagttgttgaacgatgtgggtttcgtgcaacacaatgtggaaattcgaaacgaagtgtttgtttatgaaggcgtgaggacactgaaagataatgtaaaggccatttgtccttttcttgagcgaatgccagcagatttgcatgaacagttcctggatgacttcatagacattgttatatccatgaatttgcagcaaggtgaaaataatgaggatcaaaagttcctatctccctataaattggtggtggcctatgctcgcaagactcctgaatttgtgaataatgttttcctggagcctacacatcaaaacttggttaagggaataaattaa
BBa_K817002_sequence
1
atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacc
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K2007006_sequence
1
atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacctactagagaaagaggagaaatactagatgaatcaggcctctctatatcagcacgccaaccaggtgcaaaggcacgatgccaaattgattctggacgagttcgcatcgaccatgcagtggcgatccgatggagaggatgcactcctggatgtgggttcaggatccggtaacgtgctcatggacttcgtaaagccactgctcccgattcgtggacaactggtgggcacagacatctccagccagatggtgcactatgccagtaagcattaccagcgagaggagcgcaccagattccaggttctggacattggatgcgagcgactgcctgaggagcttagcggcagatttgaccatgtcacctcgttctactgcctccattgggtgcaaaatctgaaaggagctctcggaaatatctacaatcttctgaagcccgaaggtggcgactgcctcctggcctttttggcctccaatccggtttacgaagtctataagattcttaaaacgaatgacaagtggtcgacttttatgcaggatgtggagaacttcatatccccacttcactacagtctaagtcctggcgaggagttcagtcagttgttgaacgatgtgggtttcgtgcaacacaatgtggaaattcgaaacgaagtgtttgtttatgaaggcgtgaggacactgaaagataatgtaaaggccatttgtccttttcttgagcgaatgccagcagatttgcatgaacagttcctggatgacttcatagacattgttatatccatgaatttgcagcaaggtgaaaataatgaggatcaaaagttcctatctccctataaattggtggtggcctatgctcgcaagactcctgaatttgtgaataatgttttcctggagcctacacatcaaaacttggttaagggaataaattaatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtcattaaggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactattttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggcacgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatccttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttccactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggccgatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacggtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtctggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgctatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z