BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961224 1 -35 range1961224 1 137 142 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K817001 1 BBa_K817001 FadR 2012-09-17T11:00:00Z 2015-05-08T01:13:28Z E.coli genomic sequence It's a repressor that E.coli use in sensing surrounding fatty acid concentration. false false _1075_ 0 13572 9 In stock false N/A false Tian-Shyang Shoung annotation2187738 1 FadR range2187738 1 1 720 BBa_K2007006 1 BBa_K2007006 pLR promoter expressing DmJHAMT and GFP, Lac promoter expressing FadR 2016-10-09T11:00:00Z 2016-10-10T09:59:44Z iGEM library and IDT Gene Black This circuit expresses GFP and a Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase (DmJHAMT) under a fatty acid concentration dependent manner. It also expresses FadR under the Lac promoter. false false _2474_ 27805 27805 9 false N/A false Alina Khzanyan component2491698 1 BBa_B0034 component2491711 1 BBa_R0010 component2491721 1 BBa_K817001 component2491703 1 BBa_E0040 component2491710 1 BBa_B0015 component2491719 1 BBa_B0034 component2491696 1 BBa_K817002 component2491728 1 BBa_B0015 component2491699 1 BBa_K2007002 component2491701 1 BBa_B0034 annotation2491703 1 BBa_E0040 range2491703 1 1019 1738 annotation2491699 1 BBa_K2007002 range2491699 1 99 992 annotation2491710 1 BBa_B0015 range2491710 1 1747 1875 annotation2491696 1 BBa_K817002 range2491696 1 1 72 annotation2491711 1 BBa_R0010 range2491711 1 1884 2083 annotation2491698 1 BBa_B0034 range2491698 1 81 92 annotation2491728 1 BBa_B0015 range2491728 1 2838 2966 annotation2491701 1 BBa_B0034 range2491701 1 1001 1012 annotation2491721 1 BBa_K817001 range2491721 1 2110 2829 annotation2491719 1 BBa_B0034 range2491719 1 2092 2103 BBa_K817002 1 BBa_K817002 PfadBA 2012-09-17T11:00:00Z 2015-05-08T01:13:28Z de novo synthesis Released HQ 2013 Fatty acid sensitive promoter false false _1075_ 0 13572 9 In stock false N/A false Yi-Te Lee, Chieh-Mei Wang annotation2197686 1 PfadBA range2197686 1 1 72 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K2007002 1 BBa_K2007002 Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase (DmJHAMT) 2016-10-09T11:00:00Z 2016-10-10T09:27:14Z Sherkhanov, S., Korman, T. P., Clarke, S. G., & Bowie, J. U. (2016). Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme. Scientific reports, 6. This is a coding sequence of a Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase (DmJHAMT) which catalyzed the esterification of FFAs and is specific to medium chain FFAs. false false _2474_ 27805 27805 9 Not in stock false We introduced two silent mutations to eliminate two Pst1 cut sites by changing the base pairs at position 72 from A to G and at position 558 from A to G so that we could use this part in a standard Bio Brick format. false Alina Khzanyan BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K817001_sequence 1 atggtcattaaggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactattttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggcacgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatccttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttccactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggccgatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacggtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtctggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgctatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K2007002_sequence 1 atgaatcaggcctctctatatcagcacgccaaccaggtgcaaaggcacgatgccaaattgattctggacgagttcgcatcgaccatgcagtggcgatccgatggagaggatgcactcctggatgtgggttcaggatccggtaacgtgctcatggacttcgtaaagccactgctcccgattcgtggacaactggtgggcacagacatctccagccagatggtgcactatgccagtaagcattaccagcgagaggagcgcaccagattccaggttctggacattggatgcgagcgactgcctgaggagcttagcggcagatttgaccatgtcacctcgttctactgcctccattgggtgcaaaatctgaaaggagctctcggaaatatctacaatcttctgaagcccgaaggtggcgactgcctcctggcctttttggcctccaatccggtttacgaagtctataagattcttaaaacgaatgacaagtggtcgacttttatgcaggatgtggagaacttcatatccccacttcactacagtctaagtcctggcgaggagttcagtcagttgttgaacgatgtgggtttcgtgcaacacaatgtggaaattcgaaacgaagtgtttgtttatgaaggcgtgaggacactgaaagataatgtaaaggccatttgtccttttcttgagcgaatgccagcagatttgcatgaacagttcctggatgacttcatagacattgttatatccatgaatttgcagcaaggtgaaaataatgaggatcaaaagttcctatctccctataaattggtggtggcctatgctcgcaagactcctgaatttgtgaataatgttttcctggagcctacacatcaaaacttggttaagggaataaattaa BBa_K817002_sequence 1 atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacc BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K2007006_sequence 1 atctggtacgaccagatttgacaatctggtacgaccagatgatactgagcacatcagcaggacgcactgacctactagagaaagaggagaaatactagatgaatcaggcctctctatatcagcacgccaaccaggtgcaaaggcacgatgccaaattgattctggacgagttcgcatcgaccatgcagtggcgatccgatggagaggatgcactcctggatgtgggttcaggatccggtaacgtgctcatggacttcgtaaagccactgctcccgattcgtggacaactggtgggcacagacatctccagccagatggtgcactatgccagtaagcattaccagcgagaggagcgcaccagattccaggttctggacattggatgcgagcgactgcctgaggagcttagcggcagatttgaccatgtcacctcgttctactgcctccattgggtgcaaaatctgaaaggagctctcggaaatatctacaatcttctgaagcccgaaggtggcgactgcctcctggcctttttggcctccaatccggtttacgaagtctataagattcttaaaacgaatgacaagtggtcgacttttatgcaggatgtggagaacttcatatccccacttcactacagtctaagtcctggcgaggagttcagtcagttgttgaacgatgtgggtttcgtgcaacacaatgtggaaattcgaaacgaagtgtttgtttatgaaggcgtgaggacactgaaagataatgtaaaggccatttgtccttttcttgagcgaatgccagcagatttgcatgaacagttcctggatgacttcatagacattgttatatccatgaatttgcagcaaggtgaaaataatgaggatcaaaagttcctatctccctataaattggtggtggcctatgctcgcaagactcctgaatttgtgaataatgttttcctggagcctacacatcaaaacttggttaagggaataaattaatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggtcattaaggcgcaaagcccggcgggtttcgcggaagagtacattattgaaagtatctggaataaccgcttccctcccgggactattttgcccgcagaacgtgaactttcagaattaattggcgtaacgcgtactacgttacgtgaagtgttacagcgtctggcacgagatggctggttgaccattcaacatggcaagccgacgaaggtgaataatttctgggaaacttccggtttaaatatccttgaaacactggcgcgactggatcacgaaagtgtgccgcagcttattgataatttgctgtcggtgcgtaccaatatttccactatttttattcgcaccgcgtttcgtcagcatcccgataaagcgcaggaagtgctggctaccgctaatgaagtggccgatcacgccgatgcctttgccgagctggattacaacatattccgcggcctggcgtttgcttccggcaacccgatttacggtctgattcttaacgggatgaaagggctgtatacgcgtattggtcgtcactatttcgccaatccggaagcgcgcagtctggcgctgggcttctaccacaaactgtcggcgttgtgcagtgaaggcgcgcacgatcaggtgtacgaaacagtgcgtcgctatgggcatgagagtggcgagatttggcaccggatgcagaaaaatctgccgggtgatttagccattcaggggcgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z