BBa_K2008000
1
BBa_K2008000
Bowman Birk Protease Inhibitor (BBI) active nonamer
2016-10-07T11:00:00Z
2016-10-08T03:14:00Z
Members of the lentil family produce the full BBI protein, and the genetic sequence for the peptide was sourced originally from genomic DNA.
This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI) with the addition of lysine, serine, cysteine, and isoleucine (KSCI) tag on the N-terminal end and a phenylalanine (F) tag on the C-terminal end to increase BBI solubility. This component of the composite part is fused to the N-terminal end of GFP part BBA_E0040 for visual detection, and the entire part is under the control of the constitutive E. coli promoter BBa_J23100 and the RBS BBa_B0034.
true
false
_2475_
30207
30207
9
false
We used a strong RBS and constitutive promoter to produce high levels of expression of the BBI-KSCI tagged protein fused to GFP for visualization.
false
Rachelle Varga, Nishi Patel
annotation2488260
1
BBa_J23100
range2488260
1
1
35
annotation2488262
1
BBa_B0034
range2488262
1
44
55
annotation2488264
1
BBa_E0040
range2488264
1
62
781
annotation2488263
1
GFP protein
range2488263
1
62
781
annotation2488261
1
conserved
range2488261
1
48
51
BBa_K2008000_sequence
1
aaatcatgcatttgcgcactgtcatatccggcacaatgcttttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z