BBa_K2008004
1
BBa_K2008004
pVeg->sec-TD1-BBI
2016-10-07T11:00:00Z
2016-10-08T08:25:46Z
Members of the lentil family produce the full BBI protein, and the genetic sequence for the peptide was sourced originally from genomic DNA.
This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI). A B. subtilis secretory signal peptide sequence is included to allow for secretion of the peptide directly into surrounding media. A transdermal tag (TD1, BBa_K1074000) is fused to the N-terminus of BBI to allow for diffusion of the peptide across the skin.
This coding sequence is under the control of the constitutive B. subtilis promoter pVeg (BBa_K143012) and a strong B. subtilis RBS (BBa_K780001).
Note: This part is a composite part, not a basic part, but it could not be entered as composite at this time.
false
false
_2475_
30207
30207
9
false
Bacillus subtilis-specific components were used (strong RBS and constitutive promoter) to produce high levels of expression of the BBI peptide. A secretory signal peptide sequence was included to allow for secretion of the peptide directly into surrounding media. A transdermal tag allowed for diffusion of the peptide across the skin. Codons were optimized for B. subtilis.
false
Rachelle Varga
annotation2488511
1
Sec tag
range2488511
1
126
209
annotation2488509
1
BBa_K780001
range2488509
1
98
113
annotation2488512
1
BBa_K1074000
range2488512
1
210
242
annotation2488508
1
BBa_K143012
range2488508
1
1
97
annotation2488510
1
Start codon
range2488510
1
123
125
annotation2488514
1
Double stop codon
range2488514
1
270
275
annotation2488513
1
BBI
range2488513
1
243
269
BBa_K2008004_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtatattaagaggaggagatatatataatgaatatcaagaagttcgcaaaacaggcgacagtcctgacctttaccaccgccctcttggcagggggggcgacccaggcattcgctgcttgttcttcttccccatctaagcattgtggttgcgcactgtcatatccggcacaatgctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z