LOCUS       BBa_K2010000             988 bp    DNA     linear       12-May-2024
DEFINITION  PETase (PET-degrading enzyme, origin I. sakaiensis)
ACCESSION   BBa_K2010000
VERSION     BBa_K2010000.1  
FEATURES             Location/Qualifiers
     CDS             59..928
                     /label=PETase
     promoter        1..46
                     /label=T7 Promoter
     RBS             47..58
                     /label=RBS 34
     misc_feature    929..964
                     /label=GGS Linker
     misc_feature    965..982
                     /label=His-Tag
     misc_feature    983..988
                     /label=Stop Codon
     misc_feature    59..61
                     /label=Start Codon
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaaat
       61 gaatttcccc cgtgccagtc gtctgatgca agctgccgta ttgggcgggc ttatggccgt
      121 ttccgcagcc gcgactgctc agacgaatcc atatgcacgt ggacctaacc ccacagcagc
      181 gtcattagaa gcgtcggcgg ggcctttcac tgtccgcagt ttcacggtga gccgtccgtc
      241 aggctatgga gcgggaaccg tttattatcc caccaacgca ggtggcacgg ttggtgcaat
      301 cgctattgtt cccgggtaca cagcccgcca gtcaagcatc aaatggtggg ggcctcgtct
      361 ggcaagtcat ggctttgttg tcattactat cgacacaaac agtacgcttg accaaccgag
      421 ttcgcgctct tcccagcaga tggccgccct tcgtcaagta gcatccttaa acggcacgag
      481 ttcgtctcca atctatggaa aggtggacac cgcacgcatg ggggtaatgg ggtggtctat
      541 ggggggtggg gggtcactta tcagtgcagc gaataacccg agtttaaagg cagcggcccc
      601 ccaggcccca tgggattcgt ctacaaattt ctcatctgtt accgtcccga ctcttatttt
      661 cgcatgcgag aatgatagca ttgcacctgt taatagttct gctttaccaa tctacgactc
      721 gatgagccgt aatgcaaaac aatttctgga aatcaatggc gggtcgcact cttgtgcgaa
      781 cagcggtaac tcgaatcaag ctttgatcgg gaaaaagggt gtagcatgga tgaagcgttt
      841 tatggacaac gatactcgct acagcacttt cgcttgtgag aacccgaata gtactcgcgt
      901 aagcgatttt cgcaccgcta actgctccgg ggggtcggga gggtcagggg gatcaggagg
      961 ttcgcatcac catcaccatc attagtag
//