BBa_K2010000 1 BBa_K2010000 PETase (PET-degrading enzyme, origin I. sakaiensis) 2016-06-16T11:00:00Z 2016-10-02T01:25:32Z This part comes from Ideonella sakaiensis, identified in "A bacterium that degrades and assimilates poly(ethylene terephthalate)" (Yoshida et al. 2016). PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase. false false _2477_ 30331 30331 9 false This sequence was optimized for E. coli K12 using the Codon Optimization Tool from the IDT website. false William Cho annotation2480241 1 RBS 34 range2480241 1 47 58 annotation2483551 1 GGS Linker range2483551 1 929 964 annotation2483554 1 Start Codon range2483554 1 59 61 annotation2480239 1 PETase range2480239 1 59 928 annotation2480240 1 T7 Promoter range2480240 1 1 46 annotation2483553 1 Stop Codon range2483553 1 983 988 annotation2483552 1 His-Tag range2483552 1 965 982 BBa_K2010000_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcaaaagaggagaaaatgaatttcccccgtgccagtcgtctgatgcaagctgccgtattgggcgggcttatggccgtttccgcagccgcgactgctcagacgaatccatatgcacgtggacctaaccccacagcagcgtcattagaagcgtcggcggggcctttcactgtccgcagtttcacggtgagccgtccgtcaggctatggagcgggaaccgtttattatcccaccaacgcaggtggcacggttggtgcaatcgctattgttcccgggtacacagcccgccagtcaagcatcaaatggtgggggcctcgtctggcaagtcatggctttgttgtcattactatcgacacaaacagtacgcttgaccaaccgagttcgcgctcttcccagcagatggccgcccttcgtcaagtagcatccttaaacggcacgagttcgtctccaatctatggaaaggtggacaccgcacgcatgggggtaatggggtggtctatggggggtggggggtcacttatcagtgcagcgaataacccgagtttaaaggcagcggccccccaggccccatgggattcgtctacaaatttctcatctgttaccgtcccgactcttattttcgcatgcgagaatgatagcattgcacctgttaatagttctgctttaccaatctacgactcgatgagccgtaatgcaaaacaatttctggaaatcaatggcgggtcgcactcttgtgcgaacagcggtaactcgaatcaagctttgatcgggaaaaagggtgtagcatggatgaagcgttttatggacaacgatactcgctacagcactttcgcttgtgagaacccgaatagtactcgcgtaagcgattttcgcaccgctaactgctccggggggtcgggagggtcagggggatcaggaggttcgcatcaccatcaccatcattagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z