LOCUS       BBa_K2010001            1084 bp    DNA     linear       10-May-2024
DEFINITION  ompT + PETase (PET-degrading enzyme, origin I. sakaiensis)
ACCESSION   BBa_K2010001
VERSION     BBa_K2010001.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=T7 Promoter
     misc_feature    59..118
                     /label=ompT Tag
     RBS             47..58
                     /label=RBS 34
     CDS             155..1024
                     /label=PETase
     misc_feature    1025..1060
                     /label=GGS Linker
     misc_feature    1061..1078
                     /label=His-Tag
     misc_feature    1079..1084
                     /label=Stop Codon
     misc_feature    59..61
                     /label=Start Codon
     misc_feature    119..154
                     /label=GGS Linker
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaaat
       61 gcgtgcaaaa ttattgggta tcgtgcttac cacgcctatt gcgattagct catttgcagg
      121 ggggtcggga gggtcagggg gatcaggagg ttcgatgaat ttcccccgtg ccagtcgtct
      181 gatgcaagct gccgtattgg gcgggcttat ggccgtttcc gcagccgcga ctgctcagac
      241 gaatccatat gcacgtggac ctaaccccac agcagcgtca ttagaagcgt cggcggggcc
      301 tttcactgtc cgcagtttca cggtgagccg tccgtcaggc tatggagcgg gaaccgttta
      361 ttatcccacc aacgcaggtg gcacggttgg tgcaatcgct attgttcccg ggtacacagc
      421 ccgccagtca agcatcaaat ggtgggggcc tcgtctggca agtcatggct ttgttgtcat
      481 tactatcgac acaaacagta cgcttgacca accgagttcg cgctcttccc agcagatggc
      541 cgcccttcgt caagtagcat ccttaaacgg cacgagttcg tctccaatct atggaaaggt
      601 ggacaccgca cgcatggggg taatggggtg gtctatgggg ggtggggggt cacttatcag
      661 tgcagcgaat aacccgagtt taaaggcagc ggccccccag gccccatggg attcgtctac
      721 aaatttctca tctgttaccg tcccgactct tattttcgca tgcgagaatg atagcattgc
      781 acctgttaat agttctgctt taccaatcta cgactcgatg agccgtaatg caaaacaatt
      841 tctggaaatc aatggcgggt cgcactcttg tgcgaacagc ggtaactcga atcaagcttt
      901 gatcgggaaa aagggtgtag catggatgaa gcgttttatg gacaacgata ctcgctacag
      961 cactttcgct tgtgagaacc cgaatagtac tcgcgtaagc gattttcgca ccgctaactg
     1021 ctccgggggg tcgggagggt cagggggatc aggaggttcg catcaccatc accatcatta
     1081 gtag
//