BBa_K2010001 1 BBa_K2010001 ompT + PETase (PET-degrading enzyme, origin I. sakaiensis) 2016-06-16T11:00:00Z 2016-10-17T01:23:21Z This part comes from Ideonella sakaiensis, identified in "A bacterium that degrades and assimilates poly(ethylene terephthalate)" (Yoshida et al. 2016). MHETase is the MHET(Mono(2-hydroxyethyl) terephthalate)-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for MHETase. false false _2477_ 30331 30331 9 Not in stock false This sequence was optimized for E. coli K12 using the Codon Optimization Tool from the IDT website. false William Cho annotation2483555 1 PETase range2483555 1 155 1024 annotation2480252 1 T7 Promoter range2480252 1 1 46 annotation2483556 1 GGS Linker range2483556 1 1025 1060 annotation2514862 1 GGS Linker range2514862 1 119 154 annotation2483566 1 Start Codon range2483566 1 59 61 annotation2483557 1 His-Tag range2483557 1 1061 1078 annotation2480255 1 RBS 34 range2480255 1 47 58 annotation2483558 1 Stop Codon range2483558 1 1079 1084 annotation2480254 1 ompT Tag range2480254 1 59 118 BBa_K2010001_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcaaaagaggagaaaatgcgtgcaaaattattgggtatcgtgcttaccacgcctattgcgattagctcatttgcaggggggtcgggagggtcagggggatcaggaggttcgatgaatttcccccgtgccagtcgtctgatgcaagctgccgtattgggcgggcttatggccgtttccgcagccgcgactgctcagacgaatccatatgcacgtggacctaaccccacagcagcgtcattagaagcgtcggcggggcctttcactgtccgcagtttcacggtgagccgtccgtcaggctatggagcgggaaccgtttattatcccaccaacgcaggtggcacggttggtgcaatcgctattgttcccgggtacacagcccgccagtcaagcatcaaatggtgggggcctcgtctggcaagtcatggctttgttgtcattactatcgacacaaacagtacgcttgaccaaccgagttcgcgctcttcccagcagatggccgcccttcgtcaagtagcatccttaaacggcacgagttcgtctccaatctatggaaaggtggacaccgcacgcatgggggtaatggggtggtctatggggggtggggggtcacttatcagtgcagcgaataacccgagtttaaaggcagcggccccccaggccccatgggattcgtctacaaatttctcatctgttaccgtcccgactcttattttcgcatgcgagaatgatagcattgcacctgttaatagttctgctttaccaatctacgactcgatgagccgtaatgcaaaacaatttctggaaatcaatggcgggtcgcactcttgtgcgaacagcggtaactcgaatcaagctttgatcgggaaaaagggtgtagcatggatgaagcgttttatggacaacgatactcgctacagcactttcgcttgtgagaacccgaatagtactcgcgtaagcgattttcgcaccgctaactgctccggggggtcgggagggtcagggggatcaggaggttcgcatcaccatcaccatcattagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z