LOCUS       BBa_K2010002            1090 bp    DNA     linear       22-Dec-2024
DEFINITION  pelB + PETase (PET-degrading enzyme, origin I. sakaiensis)
ACCESSION   BBa_K2010002
VERSION     BBa_K2010002.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=T7 Promoter
     RBS             47..58
                     /label=RBS 34
     misc_feature    59..124
                     /label=pelB Tag
     CDS             161..1030
                     /label=PETase
     misc_feature    1031..1066
                     /label=GGS Linker
     misc_feature    1067..1084
                     /label=His-Tag
     misc_feature    1085..1090
                     /label=Stop Codon
     misc_feature    59..61
                     /label=Start Codon
     misc_feature    125..160
                     /label=GGS Linker
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaaat
       61 gaaatacctg ctgccgaccg ctgctgctgg tctgctgctc ctcgctgccc agccggcgat
      121 ggccgggggg tcgggagggt cagggggatc aggaggttcg atgaatttcc cccgtgccag
      181 tcgtctgatg caagctgccg tattgggcgg gcttatggcc gtttccgcag ccgcgactgc
      241 tcagacgaat ccatatgcac gtggacctaa ccccacagca gcgtcattag aagcgtcggc
      301 ggggcctttc actgtccgca gtttcacggt gagccgtccg tcaggctatg gagcgggaac
      361 cgtttattat cccaccaacg caggtggcac ggttggtgca atcgctattg ttcccgggta
      421 cacagcccgc cagtcaagca tcaaatggtg ggggcctcgt ctggcaagtc atggctttgt
      481 tgtcattact atcgacacaa acagtacgct tgaccaaccg agttcgcgct cttcccagca
      541 gatggccgcc cttcgtcaag tagcatcctt aaacggcacg agttcgtctc caatctatgg
      601 aaaggtggac accgcacgca tgggggtaat ggggtggtct atggggggtg gggggtcact
      661 tatcagtgca gcgaataacc cgagtttaaa ggcagcggcc ccccaggccc catgggattc
      721 gtctacaaat ttctcatctg ttaccgtccc gactcttatt ttcgcatgcg agaatgatag
      781 cattgcacct gttaatagtt ctgctttacc aatctacgac tcgatgagcc gtaatgcaaa
      841 acaatttctg gaaatcaatg gcgggtcgca ctcttgtgcg aacagcggta actcgaatca
      901 agctttgatc gggaaaaagg gtgtagcatg gatgaagcgt tttatggaca acgatactcg
      961 ctacagcact ttcgcttgtg agaacccgaa tagtactcgc gtaagcgatt ttcgcaccgc
     1021 taactgctcc ggggggtcgg gagggtcagg gggatcagga ggttcgcatc accatcacca
     1081 tcattagtag
//