BBa_K2010002 1 BBa_K2010002 pelB + PETase (PET-degrading enzyme, origin I. sakaiensis) 2016-06-20T11:00:00Z 2016-10-17T01:26:20Z This sequence is publicly known and was privately modified. This GGS linker is a linker sequence that neutrally connects two protein domains together. The linker is composed of repetitions of glycine-glycine-serine, which are known to be neutral and not interfere with the rest of the protein. false false _2477_ 30331 30331 9 false The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences. false William Cho annotation2483563 1 His-Tag range2483563 1 1067 1084 annotation2483561 1 PETase range2483561 1 161 1030 annotation2483562 1 GGS Linker range2483562 1 1031 1066 annotation2514866 1 GGS Linker range2514866 1 125 160 annotation2483565 1 Start Codon range2483565 1 59 61 annotation2483560 1 pelB Tag range2483560 1 59 124 annotation2480261 1 T7 Promoter range2480261 1 1 46 annotation2483559 1 RBS 34 range2483559 1 47 58 annotation2483564 1 Stop Codon range2483564 1 1085 1090 BBa_K2010002_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcaaaagaggagaaaatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccggggggtcgggagggtcagggggatcaggaggttcgatgaatttcccccgtgccagtcgtctgatgcaagctgccgtattgggcgggcttatggccgtttccgcagccgcgactgctcagacgaatccatatgcacgtggacctaaccccacagcagcgtcattagaagcgtcggcggggcctttcactgtccgcagtttcacggtgagccgtccgtcaggctatggagcgggaaccgtttattatcccaccaacgcaggtggcacggttggtgcaatcgctattgttcccgggtacacagcccgccagtcaagcatcaaatggtgggggcctcgtctggcaagtcatggctttgttgtcattactatcgacacaaacagtacgcttgaccaaccgagttcgcgctcttcccagcagatggccgcccttcgtcaagtagcatccttaaacggcacgagttcgtctccaatctatggaaaggtggacaccgcacgcatgggggtaatggggtggtctatggggggtggggggtcacttatcagtgcagcgaataacccgagtttaaaggcagcggccccccaggccccatgggattcgtctacaaatttctcatctgttaccgtcccgactcttattttcgcatgcgagaatgatagcattgcacctgttaatagttctgctttaccaatctacgactcgatgagccgtaatgcaaaacaatttctggaaatcaatggcgggtcgcactcttgtgcgaacagcggtaactcgaatcaagctttgatcgggaaaaagggtgtagcatggatgaagcgttttatggacaacgatactcgctacagcactttcgcttgtgagaacccgaatagtactcgcgtaagcgattttcgcaccgctaactgctccggggggtcgggagggtcagggggatcaggaggttcgcatcaccatcaccatcattagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z