LOCUS       BBa_K2010003            1378 bp    DNA     linear       26-Nov-2024
DEFINITION  YebF + PETase (PET-degrading enzyme, origin I. sakaiensis)
ACCESSION   BBa_K2010003
VERSION     BBa_K2010003.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=T7 Promoter
     RBS             47..58
                     /label=RBS 34
     CDS             59..412
                     /label=YebF
     misc_feature    413..448
                     /label=GGS Linker
     CDS             449..1318
                     /label=PETase
     misc_feature    1355..1372
                     /label=His-Tag
     misc_feature    1319..1354
                     /label=GGS Linker
     misc_feature    59..61
                     /label=Start Codon
     misc_feature    1373..1378
                     /label=Stop Codon
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaaat
       61 gaaaaaacgt ggcgcattct taggtttact gttggtgtcc gcctgtgcgt ccgttttcgc
      121 agcaaataat gagacatcca aatccgtcac ttttccaaaa tgtgaggatt tggatgcggc
      181 gggtattgcg gccagtgtaa agcgtgacta tcagcagaac cgtgtagcac gttgggcgga
      241 cgatcagaag atcgtaggcc aggctgaccc cgtcgcatgg gtctcgttgc aagatattca
      301 gggtaaggat gataagtggt cggtgccgct tacggtacgc ggtaagagcg cggatattca
      361 ctaccaagtc tctgtagact gcaaggctgg tatggccgaa tatcagcgtc gcggggggtc
      421 gggagggtca gggggatcag gaggttcgat gaatttcccc cgtgccagtc gtctgatgca
      481 agctgccgta ttgggcgggc ttatggccgt ttccgcagcc gcgactgctc agacgaatcc
      541 atatgcacgt ggacctaacc ccacagcagc gtcattagaa gcgtcggcgg ggcctttcac
      601 tgtccgcagt ttcacggtga gccgtccgtc aggctatgga gcgggaaccg tttattatcc
      661 caccaacgca ggtggcacgg ttggtgcaat cgctattgtt cccgggtaca cagcccgcca
      721 gtcaagcatc aaatggtggg ggcctcgtct ggcaagtcat ggctttgttg tcattactat
      781 cgacacaaac agtacgcttg accaaccgag ttcgcgctct tcccagcaga tggccgccct
      841 tcgtcaagta gcatccttaa acggcacgag ttcgtctcca atctatggaa aggtggacac
      901 cgcacgcatg ggggtaatgg ggtggtctat ggggggtggg gggtcactta tcagtgcagc
      961 gaataacccg agtttaaagg cagcggcccc ccaggcccca tgggattcgt ctacaaattt
     1021 ctcatctgtt accgtcccga ctcttatttt cgcatgcgag aatgatagca ttgcacctgt
     1081 taatagttct gctttaccaa tctacgactc gatgagccgt aatgcaaaac aatttctgga
     1141 aatcaatggc gggtcgcact cttgtgcgaa cagcggtaac tcgaatcaag ctttgatcgg
     1201 gaaaaagggt gtagcatgga tgaagcgttt tatggacaac gatactcgct acagcacttt
     1261 cgcttgtgag aacccgaata gtactcgcgt aagcgatttt cgcaccgcta actgctccgg
     1321 ggggtcggga gggtcagggg gatcaggagg ttcgcatcac catcaccatc attagtag
//