BBa_K2010003 1 BBa_K2010003 YebF + PETase (PET-degrading enzyme, origin I. sakaiensis) 2016-06-20T11:00:00Z 2016-10-08T01:03:50Z This sequence is publicly known and was privately modified. This GGS linker is a linker sequence that neutrally connects two protein domains together. The linker is composed of repetitions of glycine-glycine-serine, which are known to be neutral and not interfere with the rest of the protein. This is an alternate version of BBa_K2010002, but serves the same function. false false _2477_ 30331 30331 9 false The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences. false William Cho annotation2483574 1 Stop Codon range2483574 1 1373 1378 annotation2483573 1 Start Codon range2483573 1 59 61 annotation2483572 1 GGS Linker range2483572 1 1319 1354 annotation2483571 1 His-Tag range2483571 1 1355 1372 annotation2483569 1 GGS Linker range2483569 1 413 448 annotation2483567 1 RBS 34 range2483567 1 47 58 annotation2483570 1 PETase range2483570 1 449 1318 annotation2483568 1 YebF range2483568 1 59 412 annotation2480262 1 T7 Promoter range2480262 1 1 46 BBa_K2010003_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcaaaagaggagaaaatgaaaaaacgtggcgcattcttaggtttactgttggtgtccgcctgtgcgtccgttttcgcagcaaataatgagacatccaaatccgtcacttttccaaaatgtgaggatttggatgcggcgggtattgcggccagtgtaaagcgtgactatcagcagaaccgtgtagcacgttgggcggacgatcagaagatcgtaggccaggctgaccccgtcgcatgggtctcgttgcaagatattcagggtaaggatgataagtggtcggtgccgcttacggtacgcggtaagagcgcggatattcactaccaagtctctgtagactgcaaggctggtatggccgaatatcagcgtcgcggggggtcgggagggtcagggggatcaggaggttcgatgaatttcccccgtgccagtcgtctgatgcaagctgccgtattgggcgggcttatggccgtttccgcagccgcgactgctcagacgaatccatatgcacgtggacctaaccccacagcagcgtcattagaagcgtcggcggggcctttcactgtccgcagtttcacggtgagccgtccgtcaggctatggagcgggaaccgtttattatcccaccaacgcaggtggcacggttggtgcaatcgctattgttcccgggtacacagcccgccagtcaagcatcaaatggtgggggcctcgtctggcaagtcatggctttgttgtcattactatcgacacaaacagtacgcttgaccaaccgagttcgcgctcttcccagcagatggccgcccttcgtcaagtagcatccttaaacggcacgagttcgtctccaatctatggaaaggtggacaccgcacgcatgggggtaatggggtggtctatggggggtggggggtcacttatcagtgcagcgaataacccgagtttaaaggcagcggccccccaggccccatgggattcgtctacaaatttctcatctgttaccgtcccgactcttattttcgcatgcgagaatgatagcattgcacctgttaatagttctgctttaccaatctacgactcgatgagccgtaatgcaaaacaatttctggaaatcaatggcgggtcgcactcttgtgcgaacagcggtaactcgaatcaagctttgatcgggaaaaagggtgtagcatggatgaagcgttttatggacaacgatactcgctacagcactttcgcttgtgagaacccgaatagtactcgcgtaagcgattttcgcaccgctaactgctccggggggtcgggagggtcagggggatcaggaggttcgcatcaccatcaccatcattagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z