LOCUS       BBa_K2010004            1723 bp    DNA     linear       22-Nov-2024
DEFINITION  sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
ACCESSION   BBa_K2010004
VERSION     BBa_K2010004.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=T7 Promoter
     RBS             47..58
                     /label=RBS 34
     CDS             65..778
                     /label=sfGFP
     misc_feature    779..793
                     /label=Linker
     CDS             794..1663
                     /label=PETase
     misc_feature    1664..1699
                     /label=GGS Linker
     misc_feature    1700..1717
                     /label=His-Tag
     misc_feature    65..67
                     /label=Start Codon
     misc_feature    1718..1723
                     /label=Stop Codon
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaata
       61 ctagatgtcg aaaggggagg aattatttac aggtgtagtg ccgattttgg ttgagctgga
      121 cggcgatgta aatggtcaca aattctccgt gcgtggagag ggtgaaggcg atgcgacaaa
      181 cggtaagtta acactgaaat ttatctgcac aactggtaaa ttaccagttc cttggcccac
      241 attggtcact acccttacat atggagtaca gtgctttagc cgctaccctg atcatatgaa
      301 acgccatgac ttctttaagt cagcgatgcc cgagggctat gtacaagagc gtaccatctc
      361 gtttaaggac gacgggactt ataagacacg cgcggaagta aagttcgaag gcgacacttt
      421 agttaatcgt atcgaactga agggtatcga cttcaaggaa gatgggaaca ttttgggcca
      481 caaattagaa tacaatttca acagtcataa cgtttatatc acggctgata agcagaagaa
      541 tgggattaaa gctaatttta agatccgtca taacgttgag gacggcagcg ttcagctggc
      601 agaccattat cagcaaaaca cgcccattgg agacggtccg gttcttctgc cggacaacca
      661 ctacttgtct acacagagcg tcttgtcgaa ggacccaaat gagaagcgtg accatatggt
      721 tcttctggaa tttgtcactg ccgccggcat cacacacggc atggacgaat tatataaagg
      781 gtccaagggt ccgatgaatt tcccccgtgc cagtcgtctg atgcaagctg ccgtattggg
      841 cgggcttatg gccgtttccg cagccgcgac tgctcagacg aatccatatg cacgtggacc
      901 taaccccaca gcagcgtcat tagaagcgtc ggcggggcct ttcactgtcc gcagtttcac
      961 ggtgagccgt ccgtcaggct atggagcggg aaccgtttat tatcccacca acgcaggtgg
     1021 cacggttggt gcaatcgcta ttgttcccgg gtacacagcc cgccagtcaa gcatcaaatg
     1081 gtgggggcct cgtctggcaa gtcatggctt tgttgtcatt actatcgaca caaacagtac
     1141 gcttgaccaa ccgagttcgc gctcttccca gcagatggcc gcccttcgtc aagtagcatc
     1201 cttaaacggc acgagttcgt ctccaatcta tggaaaggtg gacaccgcac gcatgggggt
     1261 aatggggtgg tctatggggg gtggggggtc acttatcagt gcagcgaata acccgagttt
     1321 aaaggcagcg gccccccagg ccccatggga ttcgtctaca aatttctcat ctgttaccgt
     1381 cccgactctt attttcgcat gcgagaatga tagcattgca cctgttaata gttctgcttt
     1441 accaatctac gactcgatga gccgtaatgc aaaacaattt ctggaaatca atggcgggtc
     1501 gcactcttgt gcgaacagcg gtaactcgaa tcaagctttg atcgggaaaa agggtgtagc
     1561 atggatgaag cgttttatgg acaacgatac tcgctacagc actttcgctt gtgagaaccc
     1621 gaatagtact cgcgtaagcg attttcgcac cgctaactgc tccggggggt cgggagggtc
     1681 agggggatca ggaggttcgc atcaccatca ccatcattag tag
//