LOCUS BBa_K2010006 1789 bp DNA linear 22-Dec-2024 DEFINITION PETase Composite (T7, His-Tag) ACCESSION BBa_K2010006 VERSION BBa_K2010006.1 FEATURES Location/Qualifiers promoter 1..46 /label=T7 Promoter RBS 47..58 /label=RBS 34 misc_feature 65..67 /label=Start Codon misc_feature 65..124 /label=ompT Tag misc_feature 125..130 /label=Linker CDS 131..844 /label=sfGFP misc_feature 845..859 /label=Linker CDS 860..1729 /label=PETase misc_feature 1730..1765 /label=GGS Linker misc_feature 1766..1783 /label=His-Tag misc_feature 1784..1789 /label=Stop Codon ORIGIN 1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaata 61 ctagatgcgt gcaaaattat tgggtatcgt gcttaccacg cctattgcga ttagctcatt 121 tgcaggatct atgtcgaaag gggaggaatt atttacaggt gtagtgccga ttttggttga 181 gctggacggc gatgtaaatg gtcacaaatt ctccgtgcgt ggagagggtg aaggcgatgc 241 gacaaacggt aagttaacac tgaaatttat ctgcacaact ggtaaattac cagttccttg 301 gcccacattg gtcactaccc ttacatatgg agtacagtgc tttagccgct accctgatca 361 tatgaaacgc catgacttct ttaagtcagc gatgcccgag ggctatgtac aagagcgtac 421 catctcgttt aaggacgacg ggacttataa gacacgcgcg gaagtaaagt tcgaaggcga 481 cactttagtt aatcgtatcg aactgaaggg tatcgacttc aaggaagatg ggaacatttt 541 gggccacaaa ttagaataca atttcaacag tcataacgtt tatatcacgg ctgataagca 601 gaagaatggg attaaagcta attttaagat ccgtcataac gttgaggacg gcagcgttca 661 gctggcagac cattatcagc aaaacacgcc cattggagac ggtccggttc ttctgccgga 721 caaccactac ttgtctacac agagcgtctt gtcgaaggac ccaaatgaga agcgtgacca 781 tatggttctt ctggaatttg tcactgccgc cggcatcaca cacggcatgg acgaattata 841 taaagggtcc aagggtccga tgaatttccc ccgtgccagt cgtctgatgc aagctgccgt 901 attgggcggg cttatggccg tttccgcagc cgcgactgct cagacgaatc catatgcacg 961 tggacctaac cccacagcag cgtcattaga agcgtcggcg gggcctttca ctgtccgcag 1021 tttcacggtg agccgtccgt caggctatgg agcgggaacc gtttattatc ccaccaacgc 1081 aggtggcacg gttggtgcaa tcgctattgt tcccgggtac acagcccgcc agtcaagcat 1141 caaatggtgg gggcctcgtc tggcaagtca tggctttgtt gtcattacta tcgacacaaa 1201 cagtacgctt gaccaaccga gttcgcgctc ttcccagcag atggccgccc ttcgtcaagt 1261 agcatcctta aacggcacga gttcgtctcc aatctatgga aaggtggaca ccgcacgcat 1321 gggggtaatg gggtggtcta tggggggtgg ggggtcactt atcagtgcag cgaataaccc 1381 gagtttaaag gcagcggccc cccaggcccc atgggattcg tctacaaatt tctcatctgt 1441 taccgtcccg actcttattt tcgcatgcga gaatgatagc attgcacctg ttaatagttc 1501 tgctttacca atctacgact cgatgagccg taatgcaaaa caatttctgg aaatcaatgg 1561 cgggtcgcac tcttgtgcga acagcggtaa ctcgaatcaa gctttgatcg ggaaaaaggg 1621 tgtagcatgg atgaagcgtt ttatggacaa cgatactcgc tacagcactt tcgcttgtga 1681 gaacccgaat agtactcgcg taagcgattt tcgcaccgct aactgctccg gggggtcggg 1741 agggtcaggg ggatcaggag gttcgcatca ccatcaccat cattagtag //