BBa_K2010006 1 BBa_K2010006 PETase Composite (T7, His-Tag) 2016-06-20T11:00:00Z 2016-10-08T01:23:25Z This part is composite of various parts from various sources. Refer to parts for details. This composite part includes PETase regulated by a T7 promoter, a strong constitutive promoter, and also a his-tag for purification. Because of the T7 promoter, this part should be used in expression cells that are compatible with this promoter. true false _2477_ 30331 30331 9 false Optimized for E. coli K12. Removed complexities for gBlock ordering. false William Cho annotation2483595 1 RBS 34 range2483595 1 47 58 annotation2483602 1 GGS Linker range2483602 1 1730 1765 annotation2483599 1 sfGFP range2483599 1 131 844 annotation2483598 1 Linker range2483598 1 125 130 annotation2483594 1 T7 Promoter range2483594 1 1 46 annotation2483601 1 PETase range2483601 1 860 1729 annotation2483597 1 ompT Tag range2483597 1 65 124 annotation2483603 1 His-Tag range2483603 1 1766 1783 annotation2483600 1 Linker range2483600 1 845 859 annotation2483596 1 Start Codon range2483596 1 65 67 annotation2483604 1 Stop Codon range2483604 1 1784 1789 BBa_K2010006_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcaaaagaggagaaatactagatgcgtgcaaaattattgggtatcgtgcttaccacgcctattgcgattagctcatttgcaggatctatgtcgaaaggggaggaattatttacaggtgtagtgccgattttggttgagctggacggcgatgtaaatggtcacaaattctccgtgcgtggagagggtgaaggcgatgcgacaaacggtaagttaacactgaaatttatctgcacaactggtaaattaccagttccttggcccacattggtcactacccttacatatggagtacagtgctttagccgctaccctgatcatatgaaacgccatgacttctttaagtcagcgatgcccgagggctatgtacaagagcgtaccatctcgtttaaggacgacgggacttataagacacgcgcggaagtaaagttcgaaggcgacactttagttaatcgtatcgaactgaagggtatcgacttcaaggaagatgggaacattttgggccacaaattagaatacaatttcaacagtcataacgtttatatcacggctgataagcagaagaatgggattaaagctaattttaagatccgtcataacgttgaggacggcagcgttcagctggcagaccattatcagcaaaacacgcccattggagacggtccggttcttctgccggacaaccactacttgtctacacagagcgtcttgtcgaaggacccaaatgagaagcgtgaccatatggttcttctggaatttgtcactgccgccggcatcacacacggcatggacgaattatataaagggtccaagggtccgatgaatttcccccgtgccagtcgtctgatgcaagctgccgtattgggcgggcttatggccgtttccgcagccgcgactgctcagacgaatccatatgcacgtggacctaaccccacagcagcgtcattagaagcgtcggcggggcctttcactgtccgcagtttcacggtgagccgtccgtcaggctatggagcgggaaccgtttattatcccaccaacgcaggtggcacggttggtgcaatcgctattgttcccgggtacacagcccgccagtcaagcatcaaatggtgggggcctcgtctggcaagtcatggctttgttgtcattactatcgacacaaacagtacgcttgaccaaccgagttcgcgctcttcccagcagatggccgcccttcgtcaagtagcatccttaaacggcacgagttcgtctccaatctatggaaaggtggacaccgcacgcatgggggtaatggggtggtctatggggggtggggggtcacttatcagtgcagcgaataacccgagtttaaaggcagcggccccccaggccccatgggattcgtctacaaatttctcatctgttaccgtcccgactcttattttcgcatgcgagaatgatagcattgcacctgttaatagttctgctttaccaatctacgactcgatgagccgtaatgcaaaacaatttctggaaatcaatggcgggtcgcactcttgtgcgaacagcggtaactcgaatcaagctttgatcgggaaaaagggtgtagcatggatgaagcgttttatggacaacgatactcgctacagcactttcgcttgtgagaacccgaatagtactcgcgtaagcgattttcgcaccgctaactgctccggggggtcgggagggtcagggggatcaggaggttcgcatcaccatcaccatcattagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z