LOCUS       BBa_K2010007            1795 bp    DNA     linear       22-Dec-2024
DEFINITION  pelB + sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
ACCESSION   BBa_K2010007
VERSION     BBa_K2010007.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=T7 Promoter
     RBS             47..58
                     /label=RBS 34
     misc_feature    65..130
                     /label=pelB Tag
     misc_feature    131..136
                     /label=Linker
     CDS             137..850
                     /label=sfGFP
     misc_feature    851..865
                     /label=Linker
     CDS             866..1735
                     /label=PETase
     misc_feature    1736..1771
                     /label=GGS Linker
     misc_feature    1772..1789
                     /label=His-Tag
     misc_feature    1790..1795
                     /label=Stop Codon
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaata
       61 ctagatgaaa tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc
      121 ggcgatggcc ggatctatgt cgaaagggga ggaattattt acaggtgtag tgccgatttt
      181 ggttgagctg gacggcgatg taaatggtca caaattctcc gtgcgtggag agggtgaagg
      241 cgatgcgaca aacggtaagt taacactgaa atttatctgc acaactggta aattaccagt
      301 tccttggccc acattggtca ctacccttac atatggagta cagtgcttta gccgctaccc
      361 tgatcatatg aaacgccatg acttctttaa gtcagcgatg cccgagggct atgtacaaga
      421 gcgtaccatc tcgtttaagg acgacgggac ttataagaca cgcgcggaag taaagttcga
      481 aggcgacact ttagttaatc gtatcgaact gaagggtatc gacttcaagg aagatgggaa
      541 cattttgggc cacaaattag aatacaattt caacagtcat aacgtttata tcacggctga
      601 taagcagaag aatgggatta aagctaattt taagatccgt cataacgttg aggacggcag
      661 cgttcagctg gcagaccatt atcagcaaaa cacgcccatt ggagacggtc cggttcttct
      721 gccggacaac cactacttgt ctacacagag cgtcttgtcg aaggacccaa atgagaagcg
      781 tgaccatatg gttcttctgg aatttgtcac tgccgccggc atcacacacg gcatggacga
      841 attatataaa gggtccaagg gtccgatgaa tttcccccgt gccagtcgtc tgatgcaagc
      901 tgccgtattg ggcgggctta tggccgtttc cgcagccgcg actgctcaga cgaatccata
      961 tgcacgtgga cctaacccca cagcagcgtc attagaagcg tcggcggggc ctttcactgt
     1021 ccgcagtttc acggtgagcc gtccgtcagg ctatggagcg ggaaccgttt attatcccac
     1081 caacgcaggt ggcacggttg gtgcaatcgc tattgttccc gggtacacag cccgccagtc
     1141 aagcatcaaa tggtgggggc ctcgtctggc aagtcatggc tttgttgtca ttactatcga
     1201 cacaaacagt acgcttgacc aaccgagttc gcgctcttcc cagcagatgg ccgcccttcg
     1261 tcaagtagca tccttaaacg gcacgagttc gtctccaatc tatggaaagg tggacaccgc
     1321 acgcatgggg gtaatggggt ggtctatggg gggtgggggg tcacttatca gtgcagcgaa
     1381 taacccgagt ttaaaggcag cggcccccca ggccccatgg gattcgtcta caaatttctc
     1441 atctgttacc gtcccgactc ttattttcgc atgcgagaat gatagcattg cacctgttaa
     1501 tagttctgct ttaccaatct acgactcgat gagccgtaat gcaaaacaat ttctggaaat
     1561 caatggcggg tcgcactctt gtgcgaacag cggtaactcg aatcaagctt tgatcgggaa
     1621 aaagggtgta gcatggatga agcgttttat ggacaacgat actcgctaca gcactttcgc
     1681 ttgtgagaac ccgaatagta ctcgcgtaag cgattttcgc accgctaact gctccggggg
     1741 gtcgggaggg tcagggggat caggaggttc gcatcaccat caccatcatt agtag
//