LOCUS       BBa_K2010008            2083 bp    DNA     linear       27-Dec-2024
DEFINITION  YebF + sfGFP + PETase
ACCESSION   BBa_K2010008
VERSION     BBa_K2010008.1  
FEATURES             Location/Qualifiers
     RBS             47..58
                     /label=RBS 34
     promoter        1..46
                     /label=T7 Promoter
     CDS             65..778
                     /label=sfGFP
     misc_feature    779..784
                     /label=Linker
     CDS             785..1138
                     /label=YebF
     misc_feature    1139..1153
                     /label=Linker
     CDS             1154..2023
                     /label=PETase
     misc_feature    2024..2059
                     /label=GGS Linker
     misc_feature    2060..2077
                     /label=His-Tag
     misc_feature    2078..2083
                     /label=Stop Codon
     misc_feature    65..67
                     /label=Start Codon
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaata
       61 ctagatgtcg aaaggggagg aattatttac aggtgtagtg ccgattttgg ttgagctgga
      121 cggcgatgta aatggtcaca aattctccgt gcgtggagag ggtgaaggcg atgcgacaaa
      181 cggtaagtta acactgaaat ttatctgcac aactggtaaa ttaccagttc cttggcccac
      241 attggtcact acccttacat atggagtaca gtgctttagc cgctaccctg atcatatgaa
      301 acgccatgac ttctttaagt cagcgatgcc cgagggctat gtacaagagc gtaccatctc
      361 gtttaaggac gacgggactt ataagacacg cgcggaagta aagttcgaag gcgacacttt
      421 agttaatcgt atcgaactga agggtatcga cttcaaggaa gatgggaaca ttttgggcca
      481 caaattagaa tacaatttca acagtcataa cgtttatatc acggctgata agcagaagaa
      541 tgggattaaa gctaatttta agatccgtca taacgttgag gacggcagcg ttcagctggc
      601 agaccattat cagcaaaaca cgcccattgg agacggtccg gttcttctgc cggacaacca
      661 ctacttgtct acacagagcg tcttgtcgaa ggacccaaat gagaagcgtg accatatggt
      721 tcttctggaa tttgtcactg ccgccggcat cacacacggc atggacgaat tatataaagg
      781 atctatgaaa aaacgtggcg cattcttagg tttactgttg gtgtccgcct gtgcgtccgt
      841 tttcgcagca aataatgaga catccaaatc cgtcactttt ccaaaatgtg aggatttgga
      901 tgcggcgggt attgcggcca gtgtaaagcg tgactatcag cagaaccgtg tagcacgttg
      961 ggcggacgat cagaagatcg taggccaggc tgaccccgtc gcatgggtct cgttgcaaga
     1021 tattcagggt aaggatgata agtggtcggt gccgcttacg gtacgcggta agagcgcgga
     1081 tattcactac caagtctctg tagactgcaa ggctggtatg gccgaatatc agcgtcgcgg
     1141 gtccaagggt ccgatgaatt tcccccgtgc cagtcgtctg atgcaagctg ccgtattggg
     1201 cgggcttatg gccgtttccg cagccgcgac tgctcagacg aatccatatg cacgtggacc
     1261 taaccccaca gcagcgtcat tagaagcgtc ggcggggcct ttcactgtcc gcagtttcac
     1321 ggtgagccgt ccgtcaggct atggagcggg aaccgtttat tatcccacca acgcaggtgg
     1381 cacggttggt gcaatcgcta ttgttcccgg gtacacagcc cgccagtcaa gcatcaaatg
     1441 gtgggggcct cgtctggcaa gtcatggctt tgttgtcatt actatcgaca caaacagtac
     1501 gcttgaccaa ccgagttcgc gctcttccca gcagatggcc gcccttcgtc aagtagcatc
     1561 cttaaacggc acgagttcgt ctccaatcta tggaaaggtg gacaccgcac gcatgggggt
     1621 aatggggtgg tctatggggg gtggggggtc acttatcagt gcagcgaata acccgagttt
     1681 aaaggcagcg gccccccagg ccccatggga ttcgtctaca aatttctcat ctgttaccgt
     1741 cccgactctt attttcgcat gcgagaatga tagcattgca cctgttaata gttctgcttt
     1801 accaatctac gactcgatga gccgtaatgc aaaacaattt ctggaaatca atggcgggtc
     1861 gcactcttgt gcgaacagcg gtaactcgaa tcaagctttg atcgggaaaa agggtgtagc
     1921 atggatgaag cgttttatgg acaacgatac tcgctacagc actttcgctt gtgagaaccc
     1981 gaatagtact cgcgtaagcg attttcgcac cgctaactgc tccggggggt cgggagggtc
     2041 agggggatca ggaggttcgc atcaccatca ccatcattag tag
//