BBa_K2012016 1 BBa_K2012016 A weak CheZ generator with fusion GFP reporter 2016-10-05T11:00:00Z 2016-11-01T10:11:24Z the CheZ coding sequence of this part is amplified from the wild type E.coli K12 MG1655 strain, the sequence is completely assistant with the sequence published on NCBI. This generator contain promotor J23117 ,RBS from BBa_J61100,functional chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate weak concentration of CheZ which can be reported by the fluorescence intensity.It is used to detect the relationship of CheZ under different strength of promotor and its swimming ability false false _2479_ 25212 32799 9 false the linker between CheZ and GFP is well concerned. Its length and sequence are optimized by using structure analysis. false Wenqi Huang annotation2486785 1 BBa_J23117 range2486785 1 1 35 annotation2486902 1 18bp linker range2486902 1 704 721 annotation2486788 1 BBa_E0040 range2486788 1 722 1438 annotation2486787 1 CheZ range2486787 1 62 703 annotation2486786 1 BBa_J61100 range2486786 1 44 55 BBa_K2012016_sequence 1 ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggggacaaactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttcaggttcatcctcgagccgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z