BBa_K2012024
1
BBa_K2012024
PcpcG2-B0034-PleD-His(6)Tag
2016-10-06T11:00:00Z
2016-10-07T03:45:56Z
PcpcG2 comes from the genome of Synechocystis PCC6803(Part BBa_K592003).
PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.(Part:BBa_K2012002)
Promoter cpcG2 is a 238bp green-light activated promoter from the genome of Synechocystis PCC6803(Part BBa_K592003).We acquire the sequence from Jeffrey Tabor in pJT119 plasmid and replace the reporter gene with PleD(Part:BBa_K2012002), a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified.
false
false
_2479_
32928
32928
9
false
The expression of PleD can be activated under the control of green light. His(6)tag is added to PleD so that the recombinant protein can be purified through Ni-chelating affinity chromatography.
false
Boyao Zhang
component2486861
1
BBa_K2012002
component2486860
1
BBa_K592003
annotation2486860
1
BBa_K592003
range2486860
1
1
238
annotation2486861
1
BBa_K2012002
range2486861
1
245
1610
BBa_K592003
1
BBa_K592003
PcpcG2 promoter, ccaR-regulated
2011-09-16T11:00:00Z
2015-05-08T01:12:48Z
This sequence can be amplified from the genome of Synechocystis PCC6803.
Reference: Hirose Y, Shimada T, Narikawa R, Katayama M, Ikeuchi M (2008)
Cyanobacteriochrome CcaS is the green light receptor that induces the expression of
phycobilisome linker protein. Proc Natl Acad Sci U S A 105: 9528-9533.
The 238 bp sequence can be directly taken from the pJT122 plasmid designed by Jeffrey Tabor.
Reference: Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene
expression in Escherichia coli. J. Mol. Biol. 405, 2, 315???324.
PcpcG2 is the green-light activated promoter from the genome of Synechocystis PCC6803. This 238 bp sequence is used by Jeffrey Tabor in pJT122 plasmid and contains the entire region upstream of cpcG2 and downstream of ccaR.
PcpcG2 is regulated by the light-activation of ccaS/ccaR. The gene downstream to this promoter is transcribed upon activation by ccaS/ccaR, via green light.
false
false
_763_
0
7929
9
Not in stock
false
This sequence was PCR-amplified directly from the pJT122 plasmid provided by Chris Voigt, the co-author of Tabor's paper on multichromatic light-sensing (Tabor et al. 2011). Due to its nature, optimizing primers to extract this sequence from the source plasmid was difficult. Recommend touchdown-PCR.
false
Lei Sun
annotation2130109
1
PcpcG2 promoter
range2130109
1
1
238
BBa_K2012002
1
BBa_K2012002
PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.
2016-09-03T11:00:00Z
2016-09-04T09:39:21Z
PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain, in its activated form.
Intracellular c-di-GMP concentration has been regulated by two functionally opposing enzymes, the diguanylate cyclases (DGCs) containing a GGDEF domain, and phosphodiesterases (PDEs) containing either an EAL or HD-GYP domain.
PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain, in its activated form.
false
false
_2479_
25212
25212
9
false
codon optimized, and could express protein in Escheriachia coli.
false
Pan Chu
BBa_K2012002_sequence
1
atgggcagcgcccggatcctcgtcgtcgacgacatcgaggccaatgtccgcctgcttgaggccaagctgacggccgagtactatgaggtctccaccgccatggacgggccgacggccctggctatggccgcgcgcgatctgcccgacatcattctgctggacgtcatgatgcccggcatggacggcttcaccgtctgccgtaagctgaaggacgatccgactacccgccacatcccggtggtgctgatcaccgcgctcgacgggcgtggcgaccgcatccagggcctggaatcgggcgcttcggacttcctgaccaagccgatcgacgacgtcatgctgttcgcccgcgtgcgcagcctgacccgcttcaagctggtgatcgacgaactgcgtcagcgcgaggcctcgggccgccgcatgggcgtgatcgccggcgccgccgcgcgcctggacggtctgggcggtcgggtgcttatcgtcgacgacaacgaacgccaggctcaacgcgtcgccgccgagctgggcgtcgaacaccgcccggtgatcgagagcgaccctgagaaggccaagatcagcgccggcggtccggtcgacctggtcatcgtcaacgctgcggccaagaacttcgatggcctgcgcttcaccgccgcgctgcggtccgaggaacgcacccgccagttgcccgtgctggccatggtcgatcccgatgatcgtggccgcatggtcaaggcgctggagatcggcgtgaacgacatcctgtcgcgcccgatcgatccgcaggaactgtccgcgcgcgtcaagacgcagatccagcgcaagcgctacactgactatctgcgcaacaatctggatcactcgctggagctggccgtcaccgaccagctgaccggcctgcacaatcgccgctacatgaccggtcagctcgactcgctggtcaagcgcgcgacactgggcggcgatccggtttcggccctgctgatcgacatcgatttcttcaagaaaatcaacgacaccttcggtcacgatatcggcgacgaggtgctgcgcgagttcgccttgcgtctggcctcgaacgtccgcgccattgatctgccttgccgctatggcggggaagagttcgtggtgatcatgcccgacaccgccctggctgacgccctgcgcatcgccgagcggatccggatgcatgtctccggctcgcccttcacggtcgcccatggccgcgaaatgctgaacgtcaccatctcgatcggcgtctcggccacggcgggcgagggcgacacgcccgaagccctgctcaagcgcgccgacgaaggcgtttatcaggccaaggcctcgggtcggaacgcggtggtcggcaaggccgccc
BBa_K592003_sequence
1
agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatct
BBa_K2012024_sequence
1
agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatcttactagatgggcagcgcccggatcctcgtcgtcgacgacatcgaggccaatgtccgcctgcttgaggccaagctgacggccgagtactatgaggtctccaccgccatggacgggccgacggccctggctatggccgcgcgcgatctgcccgacatcattctgctggacgtcatgatgcccggcatggacggcttcaccgtctgccgtaagctgaaggacgatccgactacccgccacatcccggtggtgctgatcaccgcgctcgacgggcgtggcgaccgcatccagggcctggaatcgggcgcttcggacttcctgaccaagccgatcgacgacgtcatgctgttcgcccgcgtgcgcagcctgacccgcttcaagctggtgatcgacgaactgcgtcagcgcgaggcctcgggccgccgcatgggcgtgatcgccggcgccgccgcgcgcctggacggtctgggcggtcgggtgcttatcgtcgacgacaacgaacgccaggctcaacgcgtcgccgccgagctgggcgtcgaacaccgcccggtgatcgagagcgaccctgagaaggccaagatcagcgccggcggtccggtcgacctggtcatcgtcaacgctgcggccaagaacttcgatggcctgcgcttcaccgccgcgctgcggtccgaggaacgcacccgccagttgcccgtgctggccatggtcgatcccgatgatcgtggccgcatggtcaaggcgctggagatcggcgtgaacgacatcctgtcgcgcccgatcgatccgcaggaactgtccgcgcgcgtcaagacgcagatccagcgcaagcgctacactgactatctgcgcaacaatctggatcactcgctggagctggccgtcaccgaccagctgaccggcctgcacaatcgccgctacatgaccggtcagctcgactcgctggtcaagcgcgcgacactgggcggcgatccggtttcggccctgctgatcgacatcgatttcttcaagaaaatcaacgacaccttcggtcacgatatcggcgacgaggtgctgcgcgagttcgccttgcgtctggcctcgaacgtccgcgccattgatctgccttgccgctatggcggggaagagttcgtggtgatcatgcccgacaccgccctggctgacgccctgcgcatcgccgagcggatccggatgcatgtctccggctcgcccttcacggtcgcccatggccgcgaaatgctgaacgtcaccatctcgatcggcgtctcggccacggcgggcgagggcgacacgcccgaagccctgctcaagcgcgccgacgaaggcgtttatcaggccaaggcctcgggtcggaacgcggtggtcggcaaggccgccc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z