BBa_K2012024 1 BBa_K2012024 PcpcG2-B0034-PleD-His(6)Tag 2016-10-06T11:00:00Z 2016-10-07T03:45:56Z PcpcG2 comes from the genome of Synechocystis PCC6803(Part BBa_K592003). PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.(Part:BBa_K2012002) Promoter cpcG2 is a 238bp green-light activated promoter from the genome of Synechocystis PCC6803(Part BBa_K592003).We acquire the sequence from Jeffrey Tabor in pJT119 plasmid and replace the reporter gene with PleD(Part:BBa_K2012002), a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified. false false _2479_ 32928 32928 9 false The expression of PleD can be activated under the control of green light. His(6)tag is added to PleD so that the recombinant protein can be purified through Ni-chelating affinity chromatography. false Boyao Zhang component2486861 1 BBa_K2012002 component2486860 1 BBa_K592003 annotation2486860 1 BBa_K592003 range2486860 1 1 238 annotation2486861 1 BBa_K2012002 range2486861 1 245 1610 BBa_K592003 1 BBa_K592003 PcpcG2 promoter, ccaR-regulated 2011-09-16T11:00:00Z 2015-05-08T01:12:48Z This sequence can be amplified from the genome of Synechocystis PCC6803. Reference: Hirose Y, Shimada T, Narikawa R, Katayama M, Ikeuchi M (2008) Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. Proc Natl Acad Sci U S A 105: 9528-9533. The 238 bp sequence can be directly taken from the pJT122 plasmid designed by Jeffrey Tabor. Reference: Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315???324. PcpcG2 is the green-light activated promoter from the genome of Synechocystis PCC6803. This 238 bp sequence is used by Jeffrey Tabor in pJT122 plasmid and contains the entire region upstream of cpcG2 and downstream of ccaR. PcpcG2 is regulated by the light-activation of ccaS/ccaR. The gene downstream to this promoter is transcribed upon activation by ccaS/ccaR, via green light. false false _763_ 0 7929 9 Not in stock false This sequence was PCR-amplified directly from the pJT122 plasmid provided by Chris Voigt, the co-author of Tabor's paper on multichromatic light-sensing (Tabor et al. 2011). Due to its nature, optimizing primers to extract this sequence from the source plasmid was difficult. Recommend touchdown-PCR. false Lei Sun annotation2130109 1 PcpcG2 promoter range2130109 1 1 238 BBa_K2012002 1 BBa_K2012002 PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain. 2016-09-03T11:00:00Z 2016-09-04T09:39:21Z PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain, in its activated form. Intracellular c-di-GMP concentration has been regulated by two functionally opposing enzymes, the diguanylate cyclases (DGCs) containing a GGDEF domain, and phosphodiesterases (PDEs) containing either an EAL or HD-GYP domain. PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain, in its activated form. false false _2479_ 25212 25212 9 false codon optimized, and could express protein in Escheriachia coli. false Pan Chu BBa_K2012002_sequence 1 atgggcagcgcccggatcctcgtcgtcgacgacatcgaggccaatgtccgcctgcttgaggccaagctgacggccgagtactatgaggtctccaccgccatggacgggccgacggccctggctatggccgcgcgcgatctgcccgacatcattctgctggacgtcatgatgcccggcatggacggcttcaccgtctgccgtaagctgaaggacgatccgactacccgccacatcccggtggtgctgatcaccgcgctcgacgggcgtggcgaccgcatccagggcctggaatcgggcgcttcggacttcctgaccaagccgatcgacgacgtcatgctgttcgcccgcgtgcgcagcctgacccgcttcaagctggtgatcgacgaactgcgtcagcgcgaggcctcgggccgccgcatgggcgtgatcgccggcgccgccgcgcgcctggacggtctgggcggtcgggtgcttatcgtcgacgacaacgaacgccaggctcaacgcgtcgccgccgagctgggcgtcgaacaccgcccggtgatcgagagcgaccctgagaaggccaagatcagcgccggcggtccggtcgacctggtcatcgtcaacgctgcggccaagaacttcgatggcctgcgcttcaccgccgcgctgcggtccgaggaacgcacccgccagttgcccgtgctggccatggtcgatcccgatgatcgtggccgcatggtcaaggcgctggagatcggcgtgaacgacatcctgtcgcgcccgatcgatccgcaggaactgtccgcgcgcgtcaagacgcagatccagcgcaagcgctacactgactatctgcgcaacaatctggatcactcgctggagctggccgtcaccgaccagctgaccggcctgcacaatcgccgctacatgaccggtcagctcgactcgctggtcaagcgcgcgacactgggcggcgatccggtttcggccctgctgatcgacatcgatttcttcaagaaaatcaacgacaccttcggtcacgatatcggcgacgaggtgctgcgcgagttcgccttgcgtctggcctcgaacgtccgcgccattgatctgccttgccgctatggcggggaagagttcgtggtgatcatgcccgacaccgccctggctgacgccctgcgcatcgccgagcggatccggatgcatgtctccggctcgcccttcacggtcgcccatggccgcgaaatgctgaacgtcaccatctcgatcggcgtctcggccacggcgggcgagggcgacacgcccgaagccctgctcaagcgcgccgacgaaggcgtttatcaggccaaggcctcgggtcggaacgcggtggtcggcaaggccgccc BBa_K592003_sequence 1 agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatct BBa_K2012024_sequence 1 agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatgtaacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttaggatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatcttactagatgggcagcgcccggatcctcgtcgtcgacgacatcgaggccaatgtccgcctgcttgaggccaagctgacggccgagtactatgaggtctccaccgccatggacgggccgacggccctggctatggccgcgcgcgatctgcccgacatcattctgctggacgtcatgatgcccggcatggacggcttcaccgtctgccgtaagctgaaggacgatccgactacccgccacatcccggtggtgctgatcaccgcgctcgacgggcgtggcgaccgcatccagggcctggaatcgggcgcttcggacttcctgaccaagccgatcgacgacgtcatgctgttcgcccgcgtgcgcagcctgacccgcttcaagctggtgatcgacgaactgcgtcagcgcgaggcctcgggccgccgcatgggcgtgatcgccggcgccgccgcgcgcctggacggtctgggcggtcgggtgcttatcgtcgacgacaacgaacgccaggctcaacgcgtcgccgccgagctgggcgtcgaacaccgcccggtgatcgagagcgaccctgagaaggccaagatcagcgccggcggtccggtcgacctggtcatcgtcaacgctgcggccaagaacttcgatggcctgcgcttcaccgccgcgctgcggtccgaggaacgcacccgccagttgcccgtgctggccatggtcgatcccgatgatcgtggccgcatggtcaaggcgctggagatcggcgtgaacgacatcctgtcgcgcccgatcgatccgcaggaactgtccgcgcgcgtcaagacgcagatccagcgcaagcgctacactgactatctgcgcaacaatctggatcactcgctggagctggccgtcaccgaccagctgaccggcctgcacaatcgccgctacatgaccggtcagctcgactcgctggtcaagcgcgcgacactgggcggcgatccggtttcggccctgctgatcgacatcgatttcttcaagaaaatcaacgacaccttcggtcacgatatcggcgacgaggtgctgcgcgagttcgccttgcgtctggcctcgaacgtccgcgccattgatctgccttgccgctatggcggggaagagttcgtggtgatcatgcccgacaccgccctggctgacgccctgcgcatcgccgagcggatccggatgcatgtctccggctcgcccttcacggtcgcccatggccgcgaaatgctgaacgtcaccatctcgatcggcgtctcggccacggcgggcgagggcgacacgcccgaagccctgctcaagcgcgccgacgaaggcgtttatcaggccaaggcctcgggtcggaacgcggtggtcggcaaggccgccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z