BBa_K2012028 1 BBa_K2012028 PleD-His(6)Tag 2016-10-06T11:00:00Z 2016-10-07T04:38:15Z PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.(Part:BBa_K2012002) PleD-His(6)Tag is the fusion protein of PleD(BBa_K2012002) and His-tag(BBa_J18909). PleD is a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified. false false _2479_ 32928 32928 9 false His(6)tag is added to PleD so that the recombinant protein can be purified through Ni-chelating affinity chromatography. false Boyao Zhang annotation2486903 1 PleD range2486903 1 1 1371 annotation2486927 1 His-tag range2486927 1 1372 1389 BBa_K2012028_sequence 1 atgggcagcgcccggatcctcgtcgtcgacgacatcgaggccaatgtccgcctgcttgaggccaagctgacggccgagtactatgaggtctccaccgccatggacgggccgacggccctggctatggccgcgcgcgatctgcccgacatcattctgctggacgtcatgatgcccggcatggacggcttcaccgtctgccgtaagctgaaggacgatccgactacccgccacatcccggtggtgctgatcaccgcgctcgacgggcgtggcgaccgcatccagggcctggaatcgggcgcttcggacttcctgaccaagccgatcgacgacgtcatgctgttcgcccgcgtgcgcagcctgacccgcttcaagctggtgatcgacgaactgcgtcagcgcgaggcctcgggccgccgcatgggcgtgatcgccggcgccgccgcgcgcctggacggtctgggcggtcgggtgcttatcgtcgacgacaacgaacgccaggctcaacgcgtcgccgccgagctgggcgtcgaacaccgcccggtgatcgagagcgaccctgagaaggccaagatcagcgccggcggtccggtcgacctggtcatcgtcaacgctgcggccaagaacttcgatggcctgcgcttcaccgccgcgctgcggtccgaggaacgcacccgccagttgcccgtgctggccatggtcgatcccgatgatcgtggccgcatggtcaaggcgctggagatcggcgtgaacgacatcctgtcgcgcccgatcgatccgcaggaactgtccgcgcgcgtcaagacgcagatccagcgcaagcgctacactgactatctgcgcaacaatctggatcactcgctggagctggccgtcaccgaccagctgaccggcctgcacaatcgccgctacatgaccggtcagctcgactcgctggtcaagcgcgcgacactgggcggcgatccggtttcggccctgctgatcgacatcgatttcttcaagaaaatcaacgacaccttcggtcacgatatcggcgacgaggtgctgcgcgagttcgccttgcgtctggcctcgaacgtccgcgccattgatctgccttgccgctatggcggggaagagttcgtggtgatcatgcccgacaccgccctggctgacgccctgcgcatcgccgagcggatccggatgcatgtctccggctcgcccttcacggtcgcccatggccgcgaaatgctgaacgtcaccatctcgatcggcgtctcggccacggcgggcgagggcgacacgcccgaagccctgctcaagcgcgccgacgaaggcgtttatcaggccaaggcctcgggtcggaacgcggtggtcggcaaggccgccctcgagcaccaccaccaccaccactga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z